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Positional distribution of FA in TAG of enzymatically modified borage and evening primrose oils

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Lipids

Abstract

Stereospecific analysis was carried out to establish positional distribution of FA in the TAG of DHA, EPA, and (EPA+DHA)-enriched oils. In this study, TAG of enzymatically modified oils were purified using a silicic acid column. The TAG were then subjected to positional distribution analysis using a modified procedure involving reductive cleavage with Grignard reagent. The results showed that in DHA-enriched borage oil (BO), DHA was randomly distributed over the three positions of TAG, whereas γ-linolenic acid (GLA) was mainly esterified at the sn-2 and-3 positions. In DHA-enriched evening primrose oil (EPO), however, DHA and GLA were concentrated in the sn-2 position. In EPA-enriched BO, EPA was randomly distributed over the three positions of TAG, similar to that observed for DHA. In EPA-enriched EPO, however, this FA was mainly located at the primary positions (sn-1 and sn-3) of TAG. In both oils, GLA was preferentially esterified at the sn-2 position. In (EPA+DHA)-enriched BO, EPA and DHA were mainly esterified at the sn-1 and -3 positions of TAG, whereas GLA was mainly located at the sn-2 position. In (EPA+DHA)-enriched EPO, GLA was mainly located at the sn-2 and-3 positions; EPA was preferentially esterified at the sn-1 and-3 positions, and DHA was found mainly at the sn-3 position.

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Abbreviations

AA:

arachidonic acid, 20∶4n−6

BO:

borage oil

EPO:

evening primrose oil

GLA:

γ-linolenic acid, 18∶3n−6

LA:

linoleic acid

SL:

structured lipids

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Authors and Affiliations

  1. Department of Biochemistry, Memorial University of Newfoundland, A1B 3X9, St. John's, Newfoundland, Canada

    S. P. J. Namal Senanayake & Freidoon Shahidi

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  1. S. P. J. Namal Senanayake
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  2. Freidoon Shahidi
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Correspondence to Freidoon Shahidi.

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Namal Senanayake, S.P.J., Shahidi, F. Positional distribution of FA in TAG of enzymatically modified borage and evening primrose oils. Lipids 37, 803–810 (2002). https://doi.org/10.1007/s11745-002-0964-5

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  • DOI: https://doi.org/10.1007/s11745-002-0964-5

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