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Interaction of CETP inhibitory peptide and lipoprotein substrates in cholesteryl ester transfer assay: Relationship between association properties and inhibitory activities

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Lipids

Abstract

In a previous study, CETP inhibitory peptide (3 kDa) was isolated from hog plasma. The peptide, synthesized chemically according to the amino acid sequence of the 3-kDa peptide (designated P28), showed CETP inhibitory activity both in vitro and in vivo [Cho et al. (1998) Biochim. Biophys. Acta 1391, 133–144]. We report herein further unique features of P28 when it was associated with the cholesteryl ester (CE)-donor and-acceptor lipoproteins. Lipoprotein substrates with P28 present in both HDL (as a CE-donor) and LDL (as a CE-acceptor) served as poor substrates, with CE-transfer activity decreased up to 60% compared to normal substrates without P28. P28 was found to be located in HDL fractions of hog plasma and showed the same electromobility as that visualized by PAGE on 7% polyacrylamide gel under nondenaturing conditions. Addition of apolipoprotein A-1 (apoA-1) or apoB antibody to a normal CE-transfer mixture did not alter CE-transfer activity. However, addition of apoA-1 or −B antibody to a CETP-inhibition mixture decreased the inhibitory activity of P28 by ca. 20%. Western blot analysis revealed that P28 was associated only with human and hog HDL among several lipoproteins purified from human, hog, and rabbit. CFTP-inhibition assays with various lipoprotein substrates revealed that P28 exhibited substrate-specific inhibitory activity. The inhibitory activity of P28 was highly dependent on the type of lipoprotein substrate (whether CE-donor or-acceptor); P28 inhibited CE transfer from HDL to LDL, but it did not inhibit CE transfer from HDL to HDL.

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Abbreviations

ApoA-1:

apolipoprotein A-1

apoB:

apolipoprotein B

BCA:

bicinchoninic acid

CE:

cholesteryl ester

HDLR :

reconstituted HDL

LDLR :

reconstituted LDL

LTIP:

lipid transfer inhibitor protein

PVDF:

polyvinyldifluoride

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Authors and Affiliations

  1. Cardiovascular Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Yuseong, 305-333, Daejeon, Korea

    Kyung-Hyun Cho & Song-Hae Bok

  2. Department of Food Science and Nutrition, College of Human Ecology, Kyungpook National University, 702-701, Taegu, Korea

    Myung-Sook Choi

  3. Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, 702-701, Taegu, Korea

    Ju-Young Lee & Yong Bok Park

Authors
  1. Kyung-Hyun Cho
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  2. Ju-Young Lee
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  4. Song-Hae Bok
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Correspondence to Yong Bok Park.

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Cho, KH., Lee, JY., Choi, MS. et al. Interaction of CETP inhibitory peptide and lipoprotein substrates in cholesteryl ester transfer assay: Relationship between association properties and inhibitory activities. Lipids 37, 641–646 (2002). https://doi.org/10.1007/s11745-002-0944-9

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  • DOI: https://doi.org/10.1007/s11745-002-0944-9

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