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Lipids

, Volume 37, Issue 6, pp 587–595 | Cite as

Lipid, FA, and sterol composition of New Zealand green lipped mussel (Perna canaliculus) and tasmanian blue mussel (Mytilus edulis)

  • Karen J. MurphyEmail author
  • Ben D. Mooney
  • Neil J. Mann
  • Peter D. Nichols
  • Andrew J. Sinclair
Articles

Abstract

The lipid, FA, and sterol composition of the New Zealand green lipped mussel (NZGLM, Perna canaliculus) and of the Tasmanian blue mussel (TBM, Mytilus edulis) were compared using TLC-FID and GC-MS. The respective mussel species were obtained from three different sites in both New Zealand (NZ) and Tasmania. Lipid class distribution of both mussel species was characterized by a high proportion of phospholipid (PL, 57–79%) and TG (10–25%), FFA (7–12%), and sterols (ST, 12–18%). The NZGLM had higher proportions of TG, FFA, and ST (P<0.01), whereas the TBM had a higher proportion of PL (P<0.01). There were higher proportions of total PUFA, saturated FA, n−3 FA, and hydroxy and nonmethyleneinterrupted FA (P<0.05) in the TBM compared with the NZGLM. The major FA in the NZGLM were 16∶0 (15–17%), 20∶5n-3 (14–20%), and 22∶6n-3 (11–17%). The same FA dominated lipids in the TBM, although there were significantly higher proportions of 16∶0 (P=0.000) and 22∶6 n−3 (P=0.003) and lower proportions of 20∶5n-3 (P=0.0072) in the TBM. A novel PUFA, 28∶8n-3, was detected in both mussels with higher amounts in the TBM, which probably reflects a greater dietary contribution of dinoflagellates for this species. Cholesterol was the dominant sterol in both mussels. Other major sterols included brassicasterol, 22-methylcholesterol, trans-22-dehydrocholesterol, and desmosterol. There were significant differences (P<0.05) between the NZGLM and TBM for 12 of the 20 sterols measured. Six sterols showed significant site differences for the NZGLM, and 10 for the TBM. The differences in the FA and sterol composition between the two species may be due to the diet of the NZGLM being more diatom-derived and the diet of the TBM having a greater dinoflagellate component.

Keywords

Lipid Class Mytilus Edulis Desmosterol Sterol Composition Mussel Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

20∶5n-3

EPA

22∶6n-3

DHA

HC

hydrocarbon

i 18∶0

isomer of 18∶0 FA

MUFA

monounsaturated FA

NMI

nonmethylene interrupted

NZ

New Zealand

NZGLM

New Zealand green lipped mussel

PL

phospholipid

SFA

saturated FA

ST

sterol

TBM

Tasmanian blue mussel

4,8,12-TMTD

4,8,12-trimethyl tetradecanoic acid

WE

wax ester

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References

  1. 1.
    Joseph, J.D. (1982) Lipid Composition of Marine and Estuarine Invertebrates. Part II Mollusca, Prog. Lipid Res. 21, 109–153.PubMedCrossRefGoogle Scholar
  2. 2.
    Ching, E.W.K., Siu, W.H.L., Lam, P.K., Xu, L., Zhang, Y., Richardson, B.J., and Wu, R.S.S. (2001) DNAAdduct Formation and DNA Strand Breaks in Green-Lipped Mussels (Perna viridis) Exposed to Benzo[a]pyrene: Dose-and Time-Dependent Relationships, Mar. Pollut. Bull. 42, 603–610.PubMedCrossRefGoogle Scholar
  3. 3.
    Li, S.C., Wang, W.X., and Hsieh, D.P. (2002) Effects of Toxic Dinoflagellate Alexandrium tamarense on the Energy Budgets and Growth of Two Marine Bivalves, Mar. Environ. Res. 53, 145–160.PubMedCrossRefGoogle Scholar
  4. 4.
    Chong, K., and Wang, W.X. (2001) Comparative Studies on the Biokinetics of Cd, Cr and Zn in the Green Mussel Perna viridis and the Manila Clam Ruditapes philippinarum, Environ. Pollut. 115, 107–121.PubMedCrossRefGoogle Scholar
  5. 5.
    Goode, J., and Willson, C. (1990) Seafood of Australia and New Zealand. A Comprehensive Guide to Its Preparation and Cooking, Angus and Robertson, North Ryde, New South Wales, Australia.Google Scholar
  6. 6.
    Creber, A. (1987) The Complete Australian and New Zealand Fish and Seafood Cookbook, William Heinemann, Richmond, Victoria, Australia.Google Scholar
  7. 7.
    Yearsley, G.K., Last, P.R., and Ward, R.D. (1998) Australian Seafood Domestic Species, FRDC Project 95/122, pp. 335–409, CSIRO Marine Research, Hobart, Australia.Google Scholar
  8. 8.
    Gordon, D.T. (1982) Sterols in Mollusks and Crustacea of the Pacific Northwest, J. Am. Oil Chem. Soc. 59, 536–545.Google Scholar
  9. 9.
    Nichols, P.D., Virtue, P., Mooney, B.D., Elliott, N.G., and Yearsley, G.K. (1998) Seafood the Good Food. The Oil Content and Composition of Australian Commercial Fishes, Shellfishes and Crustaceans. FRDC Project 95/122. Guide Prepared for the Fisheries Research and Development Corporation, CSIRO, Hobart, Australia.Google Scholar
  10. 10.
    Kluytmans, J.H., Boot, J.H., Oudejans, R.C.H.M., and Zandee, D.I. (1985) Fatty Acid Synthesis in Relation to Gametogenesis in the Mussel Mytilus edulis, Comp. Biochem. Physiol. 81, 959–963.CrossRefGoogle Scholar
  11. 11.
    Teshima, S., and Kanazawa, A. (1974) Biosynthesis of Sterols in Abalone, Haliotis gurneri and Mussel, Mytilus edulis, Comp. Biochem. Physiol. 47, 555–561.CrossRefGoogle Scholar
  12. 12.
    Gylling, H., and Miettinen, T.A. (1999) Cholesterol Reduction by Different Plant Stanol Mixtures and with Variable Fat Intake, Metabolism 48, 575–580.PubMedCrossRefGoogle Scholar
  13. 13.
    Naughton, J.M., O’Dea, K. and Sinclair, A.J. (1986) Animal Foods in Traditional Australian Aboriginal Diets: Polyunsaturated and Low Fat, Lipids 21, 684–690.PubMedGoogle Scholar
  14. 14.
    Nestel, P.J. (2000) Fish Oil and Cardiovascular Disease: Lipids and Arterial Function, Am. J. Clin. Nutr. 71, 228S–231S.PubMedGoogle Scholar
  15. 15.
    James, M.J., and Cleland, L.G. (1997) Dietary n−3 Fatty Acids and Therapy for Rheumatoid Arthritis, Sem. Arth. Rheum. 27, 85–97.CrossRefGoogle Scholar
  16. 16.
    James, M.J., Gibson, R.A., and Cleland, L.G. (2000) Dietary Polyunsaturated Fatty Acids and Inflammatory Mediator Production, Am. J. Clin. Nutr. 71, 343S–348S.PubMedGoogle Scholar
  17. 17.
    Lorenz, R., Weber, P.C., Szimnau, P., Heldwein, W., Strasser, T., and Loeschke, K. (1989) Supplementation with n−3 Fatty Acids from Fish Oil in Chronic Inflammatory Bowel Disease—A Randomized, Placebo-Controlled, Double-Blind Cross-over Trial, J. Intern. Med. 225, 225–232.Google Scholar
  18. 18.
    Whitehouse, M.W., Macrides, T.A., Kalafitis, N., Betts, W.H., Haynes, D.R., and Broadbent, J. (1997) Anti-inflammatory Activity of a Lipid Fraction (lyprinol) from the NZ Green-Lipped Mussel, Inflammopharmacology 5, 237–246.Google Scholar
  19. 19.
    Rainsford, K.D., and Whitehouse, M.W. (1980) Gastroprotective and Anti-inflammatory Properties of Green Lipped Mussel (Perna canaliculus) Preparation, Arzneimittelforschung/Drug Res. 30, 2128–2132.Google Scholar
  20. 20.
    Bligh, E.G., and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification, Can. J. Biochem. Physiol. 37, 911–917.PubMedGoogle Scholar
  21. 21.
    Phleger, C.F., Nelson, M.M., Mooney, B.D., and Nichols, P.D. (2001) Interannual Variations in the Lipids of the Antarctic Pteropods Clione limacina and Clio pyramidata, Comp. Biochem. Physiol. B 128, 553–564.PubMedCrossRefGoogle Scholar
  22. 22.
    Perry, G.J. (1977) Lipids in the Marine Environment, Ph.D. Thesis, Melbourne University, Victoria Australia.Google Scholar
  23. 23.
    Jeong, B.Y., Ohshima, T., Koizumi, C., and Kanou, Y. (1990) Lipid Deterioration and Its Inhibition of Japanese Oyster Crassostrea gigas During Frozen Storage, Nippon Suisan Gakkaishi 56, 2083–2091.Google Scholar
  24. 24.
    Jeong, B.Y. (1999) Changes in Molecular Species Compositions of Glycerophospholipids in the Adductor Muscle of the Giant Ezo Scallop Patinopecten yessoensis During Frozen Storage, J. Food Lipids 6, 131–147.Google Scholar
  25. 25.
    Volkman, J.K., Jeffrey, S.W., Nichols, P.D., Rogers, G.I., and Garland, C.D. (1989) Fatty Acid and Lipid Composition of 10 Species of Microalgae Used in Mariculture, J. Exp. Mar. Biol. Ecol. 128, 219–240.CrossRefGoogle Scholar
  26. 26.
    Lewis, T.E., Nichols, P.D., and McMeekin, T.A. (1999) The Biotechnological Potential of Thraustochytrids, Mar. Biotechnol. 1, 580–587.PubMedCrossRefGoogle Scholar
  27. 27.
    Mansour, M.P., Volkman, J., and Holdsworth, D.G. (1999) Very-Long Chain C28 Highly Unsaturated Fatty Acids in Marine Dinoflagellates, Phytochemistry. 50, 541–548.CrossRefGoogle Scholar
  28. 28.
    Van Pelt, C.K., Huang, M.C., Tschanz, C.L., and Brenna, J.T. (1999) An Octane Fatty Acid, 4,7,10,13,16,19,22,25-Octacosaoctaenoic Acid (28∶8n-3), Found in Marine Oils, J. Lipid Res. 40, 1501–1505.PubMedGoogle Scholar
  29. 29.
    Hallegraff, G., Nichols, P.D., Volkman, J.K., Blackburn, S., and Everitt, D. (1991) Pigments, Sterols and Fatty Acids of the Toxic Dinoflagellate Gymnodinium catenatum, J. Phycol. 27, 591–599.CrossRefGoogle Scholar
  30. 30.
    Ackman, R.G., Hooper, S.N., and Ke, P.J. (1971) The Distribution of Saturated and Isoprenoid Fatty Acids in the Lipids of Three Species of Molluscs, Littorina litorea, Crassostrea virginica, and Venus mercenaria, Comp. Biochem. Physiol. B 39, 579–587.CrossRefGoogle Scholar
  31. 31.
    Zhukova, N.V., and Svetashev, V.I. (1986) Non-methylene Interrupted Dienoic Fatty Acids in Molluscs from the Sea of Japan, Comp. Biochem. Physiol. B 83, 643–646.CrossRefGoogle Scholar
  32. 32.
    Abad, M., Ruiz, C., Martinez, D., Mosquera, G., and Sanchez, J.L. (1995) Seasonal Variations of Lipid Classes and Fatty Acids in Flat Oyster, Ostrea edulis, from San Cibran (Galicia, Spain), Comp. Biochem. Physiol. C 110, 109–118.Google Scholar

Copyright information

© AOCS Press 2002

Authors and Affiliations

  • Karen J. Murphy
    • 1
    Email author
  • Ben D. Mooney
    • 2
  • Neil J. Mann
    • 1
  • Peter D. Nichols
    • 2
  • Andrew J. Sinclair
    • 1
  1. 1.Department of Food ScienceRMIT UniversityMelbourneAustralia
  2. 2.CSIRO Marine ResearchHobartAustralia

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