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Egg ovomucin attenuates hypercholesterolemia in rats and inhibits cholesterol absorption in Caco-2 cells

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Lipids

Abstract

This experiment was designed to evaluate the effect of casein or ovomucin (OV) on the micellar solubility of cholesterol and the taurocholate binding capacity in vitro. We also evaluated the effects of casein or OV on cholesterol metabolism in rats and Caco-2 cells. OV has a significantly greater bile acid-binding capacity than that of casein in vitro. Micellar cholesterol solubility in vitro was significantly lower in the presence of OV compared to casein. The cholesterol micelles containing OV significantly suppressed cholesterol uptake by Caco-2 cells compared to the cholesterol micelles containing casein. Consistent with these in vitro findings, OV-feeding significantly increased the fecal excretion of bile acids or cholesterol compared with casein-feeding. Serum total cholesterol was significantly lower in rats fed OV than in those fed casein. The concentrations of total lipids in liver were significantly lower in the OV-fed group compared with the casein group. These results suggest that the suppression of cholesterol absorption by direct interaction between cholesterol mixed micelles and OV in the jejunal epithelia is part of the mechanism underlying the hypocholesterolemic action of OV. OV may also inhibit the reabsorption of bile acids in the ileum, thus lowering the serum cholesterol level.

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Abbreviations

EWP:

egg white protein

LTH:

β-lactoglobulin tryptic hydrolysate

OV:

ovomucin

SPH:

soy protein peptic hydrolysate

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Authors and Affiliations

  1. Department of Food Science, Faculty of Agriculture, Gifu University, 501-1193, Gifu, Japan

    Satoshi Nagaoka, Motoki Masaoka, Qing Zhang & Kenji Watanabe

  2. Research Laboratory, Q.P. Corporation, 183-0034, Fuchu, Japan

    Mineo Hasegawa

Authors
  1. Satoshi Nagaoka
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  2. Motoki Masaoka
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  3. Qing Zhang
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  4. Mineo Hasegawa
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  5. Kenji Watanabe
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Correspondence to Satoshi Nagaoka.

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Nagaoka, S., Masaoka, M., Zhang, Q. et al. Egg ovomucin attenuates hypercholesterolemia in rats and inhibits cholesterol absorption in Caco-2 cells. Lipids 37, 267–272 (2002). https://doi.org/10.1007/s11745-002-0890-6

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  • DOI: https://doi.org/10.1007/s11745-002-0890-6

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