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Exercise training-induced changes in sensitivity to endothelin-1 and aortic and cerebellum lipid profile in rats

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Lipids

Abstract

The purpose of this work was to study whether exercise training induces changes in the lipid profile of rat aorta and nervous system and in the in vitro intrinsic responsiveness of these tissues to endothelin-1 (ET-1) treatment. The exercise program performed successfully produced the characteristic metabolic alterations of the trained state. Exercise training induced a large and significant increase in the levels of both aortic ethanolamine plasmalogens (PlasEtn) and glucosylceramides. In contrast, a decrease of aortic ceramide and cholesterol levels was evoked by exercise training. ET-1 increased PlasEtn content only in sedentary animals. An exercise-induced increase in cerebellum levels of ceramides and ceramide monohexosides was found. The cerebellum ceramide content was increased by FT-1 more noticeably in sedentary rats than in trained animals. In contrast, cerebral cortex was observed to be largely insensitive to both exercise training and ET-1 treatment. It was concluded that exercise training (i) induces changes in both vascular and cerebellar lipid profiles, the former being much more pronounced than the latter, and (ii) diminishes the aortic and cerebellar sensitivity to ET-1 action.

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Abbreviations

BSS:

balanced salt solution

CH:

cholesterol

DAG:

diacylglycerol

ET-1:

endothelin-1

PlasEtn:

ethanolamine plasmalogens

PtdEtn:

phosphatidylethanolamine

PL:

phospholipids

SPH:

sphingomyelin

SPHase:

sphingomyelinase

TL:

total lipids

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Departmento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, E-28040, Madrid, Spain

    M. Dolores Aragonés, Ana Saborido & Inmaculada Fernandez

  2. Departmento de Bioquímica y Biología Molecular I, Facultad de Biología, Universidad Complutense, E-28040, Madrid, Spain

    María Marán, Jerónimo Delgado & Alicia Megías

  3. Departamento de Biología Molecular, Centro de Biología Molecular “Severo Ochoa”, Universidad Autónoma de Madrid, E-28049, Madrid, Spain

    Eduardo Latorre & R. Edgardo Catalan

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  1. Eduardo Latorre
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  2. María Marán
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  3. M. Dolores Aragonés
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Correspondence to Alicia Megías.

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Latorre, E., Marán, M., Aragonés, M.D. et al. Exercise training-induced changes in sensitivity to endothelin-1 and aortic and cerebellum lipid profile in rats. Lipids 37, 43–52 (2002). https://doi.org/10.1007/s11745-002-0862-x

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  • DOI: https://doi.org/10.1007/s11745-002-0862-x

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