The occurrence of geometric polyprenol isomers in the rubber-producing plant, Eucommia ulmoides oliver
- 240 Downloads
The chain length and geometric isomerism of polyprenols from Eucommia ulmoides Oliver were analyzed using supercritical fluid chromatography. After intensive effort to establish separation conditions for geometric isomers, a phenyl-bonded silica gel-packed column was found that cleanly separated poly-trans and-cis prenols. The presence of longchain poly-trans prenols (>9 mers) was confirmed for the first time in plants. Trans isomers were found in the leaf, seed coat, and root, but not in the bark and seed. Poly-trans prenols in this plant may act as intermediates for trans-polyisoprene biosynthesis.
KeywordsBark Seed Coat Natural Rubber Supercritical Fluid Chromatography Polymerization Degree
high-performance liquid chromatography
nuclear magnetic resonance
supercritical fluid chromatography
Unable to display preview. Download preview PDF.
- 2.Lynen, F., and Henning, U. (1960) The Biological Pathway to Natural Rubber, Angew. Chem. 72, 820–829.Google Scholar
- 9.Hendricks, S.B., Wildman, S.G., and Jones, E.J. (1946) Differentiation of Rubber and Gutta Hydrocarbon in Plant Materals, Rubber Chem. Tech. 19, 427–438.Google Scholar
- 14.Bamba, T., Fukusaki, E., Kajiyama, S., Okazawa, A., Ute, K., Kitayama, T., and Kobayashi, A. (2000) Elucidation of Polyisoprene Biosynthesis in Plants (the first report)—Analysis of Plant Polyisoprene, [Abstract] Abstracts Annual Meeting of the Japan Society for Bioscience, Biotechnology and Biochemistry, 74, p. 2.Google Scholar