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Lipids

, Volume 36, Issue 7, pp 727–732 | Cite as

The occurrence of geometric polyprenol isomers in the rubber-producing plant, Eucommia ulmoides oliver

  • Takeshi Bamba
  • Ei-ichiro Fukusaki
  • Shin-ichiro Kajivama
  • Koichi Ute
  • Tatsuki Kitayama
  • Akio Kobayashi
Articles

Abstract

The chain length and geometric isomerism of polyprenols from Eucommia ulmoides Oliver were analyzed using supercritical fluid chromatography. After intensive effort to establish separation conditions for geometric isomers, a phenyl-bonded silica gel-packed column was found that cleanly separated poly-trans and-cis prenols. The presence of longchain poly-trans prenols (>9 mers) was confirmed for the first time in plants. Trans isomers were found in the leaf, seed coat, and root, but not in the bark and seed. Poly-trans prenols in this plant may act as intermediates for trans-polyisoprene biosynthesis.

Keywords

Bark Seed Coat Natural Rubber Supercritical Fluid Chromatography Polymerization Degree 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

HPLC

high-performance liquid chromatography

IPP

isopentenyl pyrophosphate

MS

mass spectrometry

NMR

nuclear magnetic resonance

SFC

supercritical fluid chromatography

UV

ultraviolet

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Copyright information

© AOCS Press 2001

Authors and Affiliations

  • Takeshi Bamba
    • 1
  • Ei-ichiro Fukusaki
    • 1
  • Shin-ichiro Kajivama
    • 1
  • Koichi Ute
    • 2
  • Tatsuki Kitayama
    • 2
  • Akio Kobayashi
    • 1
  1. 1.Department of Biotechnology, Graduate School of EngineeringOsaka UniversityOsakaJapan
  2. 2.Department of Chemistry, Graduate School of Engineering ScienceOsaka UniversityOsakaJapan

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