Abstract
Phospholipid (PL) from both dietary sources and biliary secretions may be important in the regulation of intestinal apolipoprotein (apo) synthesis. We previously demonstrated the up-regulation of apo A-I secretion by phosphatidylcholine (PC) in a newborn piglet intestinal epithelial cell line. We hypothesized that dietary PC increases small intestinal apo A-I synthesis in vivo in the newborn piglet. Two-day-old female swine were fed by gavage for 48 h. Diets consisted of a formula containing 51% of calories as triacylglycerol providing 180 kcal/kg/24 h. The experimental group (+PC, n=7) received 1 g/L added soybean PC, and the control group (−PC, n=7) received no added PC. At the end of the study period, jejunal apo A-I, B, and A-IV synthesis was measured, and apo A-I mRNA levels were quantitated. Jejunal mucosal PI content and serum lipids and apo B and A-I levels were measured. Jejunal apo A-I synthesis was almost twice as high in the +PC group as compared to the −PC group with no difference in apo A-I mRNA levels. Jejunal content of PL was higher in the +PC group than in the −PC group. There were no differences in jejunal apo B and A-IV synthesis or serum levels of lipids and apo-lipoproteins between the two groups. Dietary PC supplementation in newborn swine up-regulated jejunal apo A-I synthesis. Apo A-IV synthesis, which is sensitive to fatty acid flux, was not significantly increased, which suggests a specific effect of PC on apo A-I synthesis. Lumenal PC may be important in the regulation of intestinal apo A-I synthesis in the neonate.
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Abbreviations
- apo:
-
apolipoprotein
- HDL:
-
high density lipoprotein
- PBS:
-
phosphate-buffered saline
- PC:
-
phosphatidylcholine
- PL:
-
phospholipid
- TCA:
-
trichloroacetic acid
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Wang, H., Du, J., Lu, S. et al. Regulation of intestinal apolipoprotein A-I synthesis by dietary phosphatidylcholine in newborn swine. Lipids 36, 683–687 (2001). https://doi.org/10.1007/s11745-001-0773-x
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DOI: https://doi.org/10.1007/s11745-001-0773-x