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Influence of fatty alcohol and other fatty acid derivatives on fatty acid uptake into rat intestinal epithelial cells

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Lipids

Abstract

We investigated the influence of various substrates on the uptake of long-chain fatty acid into IEC-6, rat intestinal epithelial cell line. The uptake of [3H]oleic acid into IEC-6 cells was a saturable function of the oleic acid concentration. Long-chain fatty acids significantly inhibited the oleic acid uptake into IEC-6 cells and shorter-chain fatty acids had little or no effect. Various fatty acid esters suppressed the oleic acid uptake into IEC-6. Fatty alcohols also inhibited oleic acid uptake into IEC-6 and the length of the carbon chain played an important role. These results suggest that long-chain fatty acid uptake was inhibited by the substrates which had a structure similar to long-chain fatty acids, especially those with a long carbon chain. At least two molecules, fatty acid translocase and fatty acid transport protein type 4, which are considered to be involved in the long-chain fatty acid transport into the cell, were expressed on IEC-6 cells, supporting the existence of the carrier-mediated system of long-chain fatty acid transport on IEC-6 cells.

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Abbreviations

BSA:

bovine serum albumin

FABPpm:

plasma membrane fatty acid-binding protein

FAT:

fatty acid translocase

FATP4:

fatty acid transport protein type 4

HBSS:

Hanks' balanced salt solution

PCR:

polymerase chain reaction

RT:

reverse transcription

TC:

sodium taurocholate

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Author notes

  1. Kaeko Murota

    Present address: Department of Nutrition, School of Medicine, The University of Tokushima, 770-8503, Tokushima, Japan

Authors and Affiliations

  1. Laboratory of Nutrition Chemistry, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan

    Kaeko Murota, Noriko Matsui, Teruo Kawada, Nobuyuki Takahashi, Takafumi Shintani, Kahori Sasaki & Tohru Fushiki

Authors
  1. Kaeko Murota
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  2. Noriko Matsui
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  3. Teruo Kawada
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  4. Nobuyuki Takahashi
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  5. Takafumi Shintani
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  6. Kahori Sasaki
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  7. Tohru Fushiki
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Correspondence to Tohru Fushiki.

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Murota, K., Matsui, N., Kawada, T. et al. Influence of fatty alcohol and other fatty acid derivatives on fatty acid uptake into rat intestinal epithelial cells. Lipids 36, 21–26 (2001). https://doi.org/10.1007/s11745-001-0663-2

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  • DOI: https://doi.org/10.1007/s11745-001-0663-2

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