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Effect of n−3 fatty acid deficiency on fatty acid composition and metabolism of aminophospholipids in rat brain synaptosomes

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Lipids

Abstract

Docosahexaenoic acid (DHA, 22∶6n−3) is one of the major polyunsaturated fatty acids esterified predominantly in aminophospholipids such as ethanolamine glycerophospholipid (EtnGpl) and serine glycerophospholipid (SerGpl) in the brain. Synaptosomes prepared from rats fed an n−3 fatty acid-deficient safflower oil (Saf) diet had significantly decreased 22∶6n−3 content with a compensatory increased 22∶5n−6 content when compared with rats fed an n−3 fatty acid-sufficient perilla oil (Per) diet. When the Saf group was shifted to a diet supplemented with safflower oil plus 22∶6n−3 (Saf+DHA) after weaning, 22∶6n−3 content was found to be restored to the level of the Per group. The uptake of [3H]ethanolamine and its conversion to [3H]EtnGpl did not differ significantly among the three dietary groups, whereas the formation of [3H]lysoEtnGpl from [3H]ethanolamine was significantly lower in the Saf group than in the other groups. The uptake of [3H]serine, its incorporation into [3H]SerGpl, and the conversion into [3H]EtnGpl by decarboxylation of [3H]SerGpl did not differ among the three dietary groups. The observed decrease in lysoEtnGpl formation associated with a reduction of 22∶6n−3 content in rat brain synaptosomes by n−3 fatty acid deprivation may provide a clue to reveal biochemical bases for the dietary fatty acids-behavior link.

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Abbreviations

ANOVA:

analysis of variance

BSS:

balanced salt solution

ChoGpl:

choline glycerophospholipid

DHA:

docosahexaenoic acid (22∶6n−3)

EtnGpl:

ethanolamine glycerophospholipid

NGF:

nerve growth factor

PBS:

phosphate-buffered saline

Per:

perilla oil

PLA:

phospholipase A

PtdIns:

phosphatidylinositol

PUFA:

polyunsaturated fatty acid

Saf:

safflower oil

SerGpl:

serine glycerophospholipid

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Applied Research Department, Harima Chemicals, Inc., 300-26, Tsukuba, Japan

    Noriaki Hata & Yoshihisa Misawa

  2. Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori, Mizuhoku, 467-8603, Nagoya, Japan

    Atsushi Ikemoto, Masayo Ohishi, Yoichi Fujii & Harumi Okuyama

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  1. Atsushi Ikemoto
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  2. Masayo Ohishi
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  3. Noriaki Hata
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  6. Harumi Okuyama
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Correspondence to Atsushi Ikemoto.

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Ikemoto, A., Ohishi, M., Hata, N. et al. Effect of n−3 fatty acid deficiency on fatty acid composition and metabolism of aminophospholipids in rat brain synaptosomes. Lipids 35, 1107–1115 (2000). https://doi.org/10.1007/s11745-000-0626-7

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  • DOI: https://doi.org/10.1007/s11745-000-0626-7

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