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Cold acclimation or grapeseed oil feeding affects phospholipid composition and mitochondrial function in duckling skeletal muscle

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Lipids

Abstract

The phospholipid fatty acid (FA) composition and functional properties of skeletal muscle and liver mitochondria were examined in cold-acclimated (CA, 4°C) ducklings. Phospholipid FA of isolated muscle mitochondria from CA birds were longer and more unsaturated than those from thermoneutral (TN, 25°C) reared ducklings. The rise in long-chain and polyunsaturated FA (PUFA, mainly 20∶4n-6) was associated with a higher State 4 respiration rate and a lower respiratory control ratio (RCR). Hepatic mitochondria, by contrast, were much less affected by cold acclimation. The cold-induced changes in phospholipid FA profile and functional properties of muscle mitochondria were reproduced by giving TN ducklings a diet enriched in grapeseed oil (GO, rich in n-6 FA), suggesting a causal relationship between the membrane structure and mitochondrial functional parameters. However, hepatic mitochondria from ducklings fed the GO diet also showed an enrichment in long-chain PUFA but opposite changes in their biochemical characteristics (lower State 4, higher RCR). It is suggested that the differential modulation of mitochondrial functional properties by membrane lipid composition between skeletal muscle and liver may depend on muscle-specific factors possibly interacting with long-chain PUFA and affecting the proton leakiness of mitochondrial membranes.

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Abbreviations

ANOVA:

analysis of variance

BAT:

brown adipose tissue

CA:

cold acclimated

FA:

fatty acid

FOG:

fast-oxidative glycolytic

GO:

grapeseed oil

MUFA:

monounsaturated fatty acid

NST:

nonshivering thermogenesis

PUFA:

polyunsaturated fatty acid

RCR:

respiratory control ratio

SFA:

saturated fatty acid

TN:

thermoneutral control duckling

U.A.R.:

Usine d'Alimentation Rationnelle

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Authors and Affiliations

  1. Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, (UMR 5578 CNRS-Université Lyon 1), Faculté des Sciences, 43 bld. du 11 Novembre 1918, bât 404, F-69622, Villeurbanne Cedex, France

    François Chaînier, Damien Roussel, Bruno Georges, Roger Meister, Jean-Louis Rouanet, Claude Duchamp & Hervé Barre

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  1. François Chaînier
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  2. Damien Roussel
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  3. Bruno Georges
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  4. Roger Meister
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  6. Claude Duchamp
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Correspondence to Roger Meister.

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Chaînier, F., Roussel, D., Georges, B. et al. Cold acclimation or grapeseed oil feeding affects phospholipid composition and mitochondrial function in duckling skeletal muscle. Lipids 35, 1099–1106 (2000). https://doi.org/10.1007/s11745-000-0625-8

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  • DOI: https://doi.org/10.1007/s11745-000-0625-8

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