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Cu2+-induced low density lipoprotein peroxidation is dependent on the initial O2 concentration: An O2 consumption study

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Lipids

Abstract

Atherosclerotic plaques form in the arterial intima, where low density lipoprotein (LDL) is thought to be oxidatively modified at sites which may contain catalytic amounts of copper in the presence of low O2 tension. We have investigated O2 consumption during LDL peroxidation induced by Cu2+ ions in vitro and found two phases: a lag phase followed by a phase of rapid O2 consumption. The length of the lag phase was dependent on Cu2+ and on initial O2 concentrations; increasing either decreased the lag time; however, LDL concentration had no effect. LDL-induced Cu2+ reduction, however, was not affected by low initial O2 concentrations, suggesting that O2 is not required for LDL-mediated reduction of Cu2+. Following the lag phase O2 consumption was dependent upon LDL or initial O2 concentrations; Cu2+ concentrations had little effect, suggesting that the propagation phase is more dependent on the presence of LDL lipids and O2 as substrates for the reaction. In summary, LDL peroxidation takes place in the presence of Cu2+ at low O2 tension; however, the reaction is dependent upon initial O2 concentrations; increases shorten the lag phase and accelerate O2 consumption.

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Abbreviations

apoB:

apoprotein B

FPLC:

fast protein liquid chromatography

LDL:

low density lipoprotein

PBS:

phosphate-buffered saline

UV:

ultraviolet

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Author notes

  1. Peter J. Sadler

    Present address: Department of Chemistry, University of Edinburgh, West Mains Road, EH9 3JJ, Edinburgh, UK

Authors and Affiliations

  1. Department of Chemistry, Birkbeck College, University of London, Gordon House and Christopher Ingold Laboratories, WC1H OPP, London, UK

    John K. Lodge & Peter J. Sadler

  2. Linus Pauling Institute, Oregon State University, 97331-6512, Corvallis, Oregon

    Maret G. Traber

Authors
  1. John K. Lodge
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  2. Maret G. Traber
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  3. Peter J. Sadler
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Correspondence to John K. Lodge.

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Lodge, J.K., Traber, M.G. & Sadler, P.J. Cu2+-induced low density lipoprotein peroxidation is dependent on the initial O2 concentration: An O2 consumption study. Lipids 35, 1087–1092 (2000). https://doi.org/10.1007/s11745-000-0623-x

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  • DOI: https://doi.org/10.1007/s11745-000-0623-x

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