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Composition of cecal bile acids in ex-germfree mice inoculated with human intestinal bacteria

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Lipids

Abstract

Germfree (GF) mice were orally inoculated with human fecal suspension or various components of human fecal microbiota. Three weeks after the inoculation, cecal bile acid composition of these mice was examined. More than 80% of total bile acids was deconjugated in the cecal contents of ex-GF mice associated with human fecal dilutions of 10−2 or 10−6, or anaerobic growth from a dilution of 10−6. In these ex-GF mice, deoxycholic acid accounted for about 20% of total bile acids. In the cecal contents of ex-GF mice associated only with clostridia, unconjugated bile acids made up less than 40% of total bile acids, about half of those in other ex-GF groups. However, the percentage of deoxycholic acid in these mice was the same as that in the other groups. These results indicate that dominant anaerobic bacterial combination is efficient for deconjugation of primary bile acids, and that clostridia in the human feces may play an important role in 7α-dehydroxylation of unconjugated primary bile acids in the intestine.

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Abbreviations

EG:

Eggerth Gagnon

GB:

gnotobiotic

GF:

germfree

PHP-LH-20:

piperidinohydroxypropyl Sephadex-LH-20

SPF:

specific pathogenfree

TS:

Trypticase soy

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Authors and Affiliations

  1. Shionogi Research Laboratories, Toyonaka, 561-0825, Osaka, Japan

    Kazuo Kuruma

  2. The Cell Research Foundation, 541-0045, Osaka, Japan

    Kiyohisa Uchida

  3. Laboratory of Veterinary Public Health, Graduate School of Agriculture and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan

    Seiko Narushima & Kikuji Iton

Authors
  1. Seiko Narushima
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  2. Kikuji Iton
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  3. Kazuo Kuruma
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  4. Kiyohisa Uchida
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Correspondence to Kikuji Iton.

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Narushima, S., Iton, K., Kuruma, K. et al. Composition of cecal bile acids in ex-germfree mice inoculated with human intestinal bacteria. Lipids 35, 639–644 (2000). https://doi.org/10.1007/s11745-000-0568-0

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  • DOI: https://doi.org/10.1007/s11745-000-0568-0

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