Abstract
The transport of palmitic acid (PA) across planar lipid bilayer membranes was measured using a high specific activity [14C]palmitate as tracer for PA. An all-glass trans chamber was employed in order to minimize adsorbance of PA onto the surface. Electrically neutral (diphytanoyl phosphatidylcholine) and charged (Azolectin) planar bilayers were maintained at open electric circuit. We found a permeability to PA of (8.8±1.9)×10−6 cm s−1 (n=15) in neutral and of (10.3±2.2)×10−6 cm s−1 (n=5) in charged bilayers. These values fall within the order of magnitude of those calculated from desorption constants of PA in different vesicular systems. Differences between data obtained from planar and vesicular systems are discussed in terms of the role of solvent, radius of curvature, and pH changes.
Similar content being viewed by others
Abbreviations
- cpm:
-
counts per minute
- DPPC:
-
diphytanoyl phosphatidylcholine
- FA:
-
fatty acid
- GUV:
-
giant unilamellar vesicle
- LUV:
-
large unilamellar vesicle
- PA:
-
palmitic acid
- PBM:
-
planar bilayer membranes
- SUV:
-
small unilamellar vesicle
- Trizma:
-
tris(hydroxymethyl)aminomethane
- UV:
-
unilamellar vesicles
References
Hannigan, G.E., and Williams, B.R.G. (1991) Signal Transduction by Interferon-α Through Arachidonic Acid Metabolism, Science 251, 204–207.
Ordway, R.W., Walsh, J.V., Jr., and Singer, J.J. (1989) Arachidonic Acid and Other Fatty Acids Directly Activate Potassium Channels in Smooth Muscle Cells, Science 244, 1176–1179.
Philipson, K.D., and Ward, R. (1985) Effects of Fatty Acids on Na+−Ca2+ Exchange and Ca2+ Permeability of Cardiac Sarcolemmal Vesicles, J. Biol. Chem. 256, 9666–9671.
Bihain, B.E., Deckelbaum, R.J., Yen, F.T., Gleeson, A.M., Carpentier Y.A., and White, L.D. (1989) Unesterified Fatty Acids Inhibit the Binding of Low Density Lipoproteins to the Human Fibroblast Low Density Lipoprotein Receptor, J. Biol. Chem. 264, 17316–17323.
Skulachev, V.P. (1991) Fatty Acid Circuit as a Physiological Mechanism of Uncoupling of Oxidative Phosphorylation. Review, FEBS Lett. 294, 158–162.
Kamp, F., and Hamilton, J.A. (1993) Movement of Fatty Acids, Fatty Acid Analogues, and Bile Acids Across Phospholipid Bilayers, Biochemistry 32, 11074–11086.
Kamp, F., Zakim, D., Zhang, F., Noy, N., and Hamilton, J.A. (1995) Fatty Acid Flip-Flop in Phospholipid Bilayers is Extremely Fast, Biochemistry 34, 11928–11937.
Kleinfeld, A.M., Chu, P., and Romero, C. (1997) Transport of Long-Chain Fatty Acids Across Lipid Bilayer Membranes Indicates That Transbilayer Flip-Flop Is Rate Limiting, Biochemistry 36, 14146–14158.
Gutknecht, J. (1988) Proton Condutance Caused by Long-Chain Fatty Acids in Phospholipid Bilayer Membranes, J. Membrane Biol. 106, 83–93.
Mailman, D., and Rose, C. (1990) Binding and Solubility of Oleic Acid to Laboratory Materials: a Possible Artifact, Life Sci. 47, 1737–1744.
Kagawa, Y., and Racker, E. (1966) Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation IX. Reconstruction of Oligomycin-Sensitive Adenosine Triphosphatase, J. Biol. Chem. 241, 2467–2474.
McLaughlin, S.G.A., Szabo, G., and Eisenman, G. (1971) Divalent Ions and the Surface Potential of Charged Phospholipid Membranes, J. Gen. Physiol. 58, 667–687.
Mueller, P., Rudin, H.T., Tien, T., and Wescott, W.C. (1963) Methods for the Formation of Single Bimolecular Lipid Membranes in Aqueous Solutions, J. Phys. Chem. 67, 534–535.
Procopio, J., Varanda, W.A., and Fornes, J.A. (1982) A Quartz Cell for Studying Planar Lipid Bilayers, Biochim. Biophys. Acta 688, 808–810.
Hamilton, J.A. (1998) Fatty Acid Transport: Difficult or Easy? J. Lipid Res. 39, 467–481.
Zhang, F., Kamp, F., and Hamilton, J.A. (1996) Dissociation of Long and Very Long Chain Fatty Acids from Phospholipid Bilayers, Biochemistry 35, 16055–16060.
Cevc, G., and Marsh, D. (1987) Phospholipid Bilayers: Physical Principles and Models, Vol. 5 of Cell Biology: A Series of Monographs (Bittar, E.E., ed.), pp. 182–183, John Wiley & Sons, New York.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Romano-Fontes, L.G., Curi, R., Peres, C.M. et al. Fatty acid transport across lipid bilayer planar membranes. Lipids 35, 31–34 (2000). https://doi.org/10.1007/s11745-000-0491-4
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11745-000-0491-4