Journal of Surfactants and Detergents

, Volume 20, Issue 5, pp 1173–1187 | Cite as

Biosafety Evaluation of Three Sodium Lauryl N-Amino Acids Synthesized from Silk Industrial Waste in Mice

  • Biao Ding
  • Liang-Ze Wan
  • Yu-Qing ZhangEmail author
Original Article


In this study, three amino acid mixtures recovered from silk industry waste were reacted with lauryl chloride to synthesize three sodium laurel N-amino acid surfactants. The three types of anionic surfactants are referred to as silk fibroin surfactant, silk sericin surfactant, and silkworm pupae surfactant. All three surfactants showed good surface properties. To evaluate biological safety, we added these three surfactants to the normal diet of mice for 8 weeks. Blood indices, blood lipid, antioxidant capacity (GSH-PX, MDA, T-AOC, and T-SOD), and lipid metabolism key enzyme mRNA (PPAR-γ, SREBP, FASN, and C/EBP-α) levels in the liver were measured. No evident histopathological changes were found in the liver and kidney. The results showed no significant difference in their indexes between the surfactant-fed and normal diet-fed mice. Thus, the three amino acid surfactants are biologically safe and can be used in daily chemical cleaning products. These surfactants will not only help to reduce wastage of resources and damage to the environment, but also yield economic benefits to the silk industry in the future.


Surfactant Sodium lauryl N-amino acids Biological safety Fibroin Sericin Silkworm pupae Mice 



The authors gratefully acknowledge the earmarked fund (CARS-22-ZJ0504) for China Agriculture Research System (CARS) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, P. R. China.


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Copyright information

© AOCS 2017

Authors and Affiliations

  1. 1.Silk Biotechnology Laboratory, School of Biology and Basic Medical SciencesSoochow UniversitySuzhouPeople’s Republic of China

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