Abstract
Results from surface tension measurements on mixed solutions of the protein bovine serum alburnin (BSA) and an anionic surfactant (SDS: sodium dodecyl sulfate) suggested that at an air-liquid interface, adsorption was affected by the protein-surfactant interaction and by the relative concentration of each component in solution. Two plateaus corresponding to the critical aggregation concentration (CAC) and the critical micelle concentration (CMC) of SDS, respectively, were observed in the surface tension isotherms of SDS in the presence of BSA. The CAC and CMC depended on the concentration of BSA. Effects of SDS concentration on the conformational changes of BSA were investigated by Fourier transform-Raman spectroscopy. The results showed that the contents of α-helix decreased while the contents of random coil increased. The presence of the anionic surfactant SDS had a negative influence on the way that proteins adsorb at an air-liquid interface, leading to the change of behavior of protein-stabilized film.
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Abbreviations
- BSA:
-
bovine serum albumin
- CAC:
-
critical aggregation concentration
- CMC:
-
critical micelle concentration
- FT-Raman:
-
Fourier transform-Raman spectroscopy
- R :
-
mole ratio of [SDS]/[BSA]
- SDS:
-
sodium dodecyl sulfate
References
Liu, H.-Z., W.-J. Wang, and J.-Y. Chen, Effects of Surfactants on Emulsification and Secondary Structure of Lysozyme in Aquous Solution, Biochem. Eng. J. 2:187 (1998).
Wei, X.-F., and H.-Z. Liu, Relationship Between Foaming Properties and Solution Properties of Protein/Nonionic Surfactant Mixtures, J. Surfact. Deterg. 3:491 (2000).
Tanford, C., Hydrophobic Free Energy, Micelle Formation and the Association of Proteins with Amphiphiles, J. Mol. Biol. 67:59 (1972).
Reynolds, J.A., and C. Tanford, The Gross Conformation of Protein-Sodium Dodecyl Sulfate Complexes, J. Biol. Chem. 245: 5161 (1970).
Ananthapadmanabhan, K.P., Protein-Surfactant Interactions, in Interactions of Surfactants with Polymers and Proteins, edited by E.D. Goddard, and K.P. Ananthapadrianbhan, GRC Press UK, London, 1993, pp. 319.
Turro, N.J., X.-G. Lei, K.P. Ananthapadmanabhan, and M. Aronson, Spectroscopic Probe Analysis of Protein-Surfactant Interactions: The BSA/SDS System, Langmuir 11:2525 (1995).
Vasilescu, M., D. Angelescu, M. Almgren, and A. Valstar, Interactions of Globular Proteins with Surfactants Studied with Fluorescence Probe Methods, Langmuin 15:2635 (1999).
Wilde, P.J., M.R. Rodriguez Nino, D.C. Clark, and J.M. Rodriguez Patino, Molecular Diffusion and Drainage of Thin Liquid Films Stabilized by Bovine Serum Albumin-Tween 20 Mixtures in Aqueous Solutions of Ethanol and Sucrose, Langmuir 13:7151 (1997).
Coke, M., P.J. Wilde, E.J. Russell, and D.C. Clark, The Influence of Surface Composition and Molecular Diffusion on the Stability of Foams Formed from Protein/Surfactant Mixtures, J. Colloid Interface Sci. 138:489 (1990).
Kragel, J., R. Wustneck, F. Husband, P.J. Wild, A.V. Makievski, D.O. Grigoriev, and J.B. Li, Properties of Mixed Protein/Surfactant Adsorption Layers, Colloids Surf. B 12:399 (1999).
Murray, B.S., A. Ventura, and C. Lallemant, Dilatational Rheology of Protein + Nonionic Surfactant Films at Air-Water and Oil-Water Interface, Colloids Surf. A 143:211 (1998).
Rodriguez Patino, J.M., M.R. Rodriguez Nino, and J.M.G. Alvarez, Interfacial and Foaming Characteristics of Protein-Lipid Systems, Food Hydrocolloids 11:49 (1997).
Németh, Zs., Gy. Racz, and K. Koczo, Antifoaming Action of Polyoxyethylene-Polyoxypropylene-Polyoxyethylene-Type Triblock Copolymers on BSA Foams, Colloids Surf. A. 127:151 (1997).
Green, R.J., T.J. Su, H. Joy, and J.R. Lu, Interaction of Lysozyme and Sodium Dodecyl Sulfate at the Air-Liquid Interface, Langmuir 16:5797 (2000).
Purcell, I.P., J.R. Lu, R.K. Thomas, A.M. Howe, and J. Penfold, Adsorption of Sodium Dodecyl Sulfate at the Surface of Aqueous Solutions of Poly(vinylpyrrolidone) Studied by Neutron Reflection, Langmuir 14:1637 (1998).
Lu, J.R., A. Morocco, T.J. Su, R.K. Thomas, and J. Penfold, Adsorption of Dodecyl Sulfate Surfactants with Monovalent Metal Counterions at the Air-Water Interface Studied by Neutron Reflection and Surface Tension, J. Colloid Interface Sci. 158:303 (1993).
Graham, D.E., and M.C. Phillips, Proteins at Liquid Interfaces: II Adsorption Isotherms, J. Colloid Interface Sci. 70:415 (1979).
Jones, M.N., A Theoretical Approach to the Binding of Amphipathic Molecules to Globular Proteins, Biochem. J. 151:109 (1975).
Mackie, A.R., A.P. Gunning, P.J. Wilde, and V.J. Morris, Orogenic Displacement of Protein from the Air/Water Interface, J. Colloid Interface Sci. 210:157 (1999).
Mackie, A.R., A.P. Gunning, P.J. Wilde, and V.J. Morris, Competitive Displacement of β-Lactoglobulin from the Air/Water Interface by Sodium Dodecyl Sulfate, Langmuir 16:8176 (2000).
Susi, H., and D.M. Byler, Fourier Deconvolution of the Amide I Raman Band of Proteins as Related to Conformation, Appl. Spectrosc. 42:819 (1988).
Servagent-Noinvile, S., M. Revault, H. Quiquampoix, and M.H. Baron. Confformational Changes of Bovine Serum Albumin Induced by Adsorption on Different Clay Surfaces: FTIR Analysis, J. Colloid Interface Sci. 221:273 (2000).
Byler, D.M., and D.M. Susi, Examination of Secondary Structure of Proteins by Deconvolved FTIR Spectra, Biopolymers 25: 469 (1986).
Razumovsky, L., and S. Damodaran, Surface Activity-Compressibility Relationship of Proteins at the Air-Water Interface, Langmuir 15:1392 (1999).
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Wei, X., Chang, Z. & Liu, H. Influence of sodium dodecyl sulfate on the characteristics of bovine serum albumin solutions and foams. J Surfact Deterg 6, 107–112 (2003). https://doi.org/10.1007/s11743-003-0252-7
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DOI: https://doi.org/10.1007/s11743-003-0252-7