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Microbial survival and odor in laundry

Journal of Surfactants and Detergents

Abstract

The survival and distribution of microflora during laundering at 30 or 40°C in commercial U.S. and European Union (E.U.) detergents were determined in laboratory wash experiments. Four test strains—Staphylococcus epidermidis, S. aureus, Escherichia coli, and Pseudomonas aeruginosa—were evaluated on cotton textile. A significant survival and transfer between textiles were found for all four test strains washed in E.U. and U.S. color detergents (without bleach), whereas no survival was observed in bleach-containing detergents. Gram-negative strains generally survived in greater numbers than Gram-positive strains. A greater survival was observed in U.S. detergents at U.S. conditions (30°C, 12 min) than in E.U. detergents at E.U. conditions (40°C, 30 min). The adhesion of odorants to cotton and polyester textiles during washing and drying was studied using six previously identified odorants in laundry [ethylbutanoate, (Z)-4-heptenal, (E)-2-nonenal, 3-methylbutanoic acid, 2-methoxyphenol (guaiacol) and 4-methyloctanoic acid]. All odorants were effectively removed from cotton during wash, whereas the odorants were more strongly associated with polyester fibers. During wash, hydrophobic odorants [(Z)-4-heptenal, (E)-2-nonenal, and guaiacol] adhered more strongly to polyester than the acids. The odor formed by surviving skin microflora attached to textiles soiled with human sebum and sweat after laundering at 30°C was studied by sensory evaluation and aroma extract dilution analysis. Intensive odor was formed in both cotton and polyester textiles during prolonged drying. Generally, the odor formation in cotton swatches and the bacterial count of the wash liquor from cotton swatches were greater than the odor formation and bacterial count from polyester swatches. Odorants with animal notes (branched fatty acids) dominated the odor profile after prolonged drying. Polyester swatches possessed a more complex odor profile than cotton; in particular, aldehydes were more dominating in polyester than in cotton. A high-impact and malodorous component, 3-methylindole, was formed during prolonged drying in cotton. The study demonstrates that microbial odor formation is a dominating factor determining the odor impression of laundered cotton and polyester textiles dried under slow drying conditions. The initial soiling with aromatic components has an additional impact on the odor profile of polyester textiles after wash, due to strong adherence of odorants during the wash cycle.

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References

  1. Teng, A., The Detergent Market, Happi 37(1):72 (2000).

    Google Scholar 

  2. Legnani, P.P., and E. Leoni, Factors Affecting the Biological Contamination of Commercial Washing Machines, Zentralbl. Hyg. Umweltmed. 200:319 (1997).

    CAS  Google Scholar 

  3. Labows, J.N., J.T. Reilly, J.J. Leyden, and G. Preti, in Antiperspirants and Deodorants, 2nd edn., edited by K. Laden, Marcel Dekker, New York, 1999, p. 59.

    Google Scholar 

  4. Weide, M.R., and M. Heinzel, Situation Analysis of Hygiene in the Home, SÖFW-J. 126:6 (2000).

    Google Scholar 

  5. Whalley, G., Detergent Powder Bleaches, Happi 32(11):61 (1995).

    CAS  Google Scholar 

  6. Kehrli, J.-P., and B. Mebes, Die hygienische ausrüstung funktioneller bekleidung, Textilveredlung 25:276 (1990).

    CAS  Google Scholar 

  7. Payne, J.D., and D.W. Kudner, A Durable Antiodor Finish for Cotton Textiles, Text. Chem. Color. 28:28 (1996).

    CAS  Google Scholar 

  8. Leyden, J.J., K.J. McGinley, E. Hölzle, M.D.J. Labows, and A.M. Kligman, The Microbiology of the Human Axilla and Its Relationship to Axillary Odor, J. Invest. Dermatol. 77:413 (1981).

    Article  CAS  Google Scholar 

  9. Froebe, C., A. Simone, and E. Eigen, Axillary Malodor Production: A New Mechanism, J. Soc. Cosmet. Chem. 41:173 (1990).

    CAS  Google Scholar 

  10. Labows, J., K. McGinley, and A. Kligman, Perspectives on Axillary Odor, Ibid. 34:193 (1982).

    Google Scholar 

  11. Bird, S., and D.B. Gower, Axillary 5α-Androst-16-en-3-one, Cholesterol and Squalene in Men: Preliminary Evidence for 5α-Androstenone Being a Product of Bacterial Action, J. Steroid Biochem. 17:517 (1982).

    Article  CAS  Google Scholar 

  12. Makin, S.A., and M.R. Lowry, Deodorant Ingredients, in Antiperspirants and Deodorants, 2nd edn., edited by K. Laden, Marcel Dekker, New York, 1999, p. 169.

    Google Scholar 

  13. Jordanov, D., H. Luther, T. Schewe, J. Slapke, and T. Strumpf, Deodorants Comprising Lipoxygenase Inhibitors, Patent DD 293,958 (1991).

    Google Scholar 

  14. Munk, S., P. Münch, L. Stahnke, J. Adler-Nissen, and P. Schieberle, Primary Odorants of Laundry Soiled with Sweat/Sebum: Influence of Lipase on the Odor Profile, J. Surfact. Deterg. 3:505 (2000).

    CAS  Google Scholar 

  15. Gibson, L.F., and T.T. Khoury, Storage and Survival of Bacteria by Ultra-Freeze, Lett. Appl. Microbiol. 3:127 (1986).

    Google Scholar 

  16. Johansen, C., B.K. Bredtved, and S. Møller, Use of Conductance Measurements for Determination of Enzymatic Degradation of Microbial Biofilm, in Biofilms, edited by R.J. Doyle, Academic Press, New York, 1999, Vol. 310, p. 353.

    Chapter  Google Scholar 

  17. Johansen, C., P. Falholt, and L. Gram, Enzymatic Removal and Disinfection of Bacterial Biofilms, Appl. Environ. Microbiol. 63: 3724 (1997).

    CAS  Google Scholar 

  18. Owens, J.D., D.S. Thomas, P.S. Thompson, and J.W. Timmerman, Indirect Conductimetry: A Novel Approach to the Conductimetric Enumeration of Microbial Populations, Lett. Appl. Microbiol. 9:245 (1989).

    CAS  Google Scholar 

  19. Engel, W., W. Bahr, and P. Schieberle, Solvent Assisted Flavour Evaporation—A New and Versatile Technique for the Careful and Direct Isolation of Aroma Compounds from Complex Food Matrices, Eur. Food Res. Technol. 209:237 (1999).

    Article  CAS  Google Scholar 

  20. Bemelmans, J.M.H., Review of Isolation and Concentration Techniques, in Progress in Flavour Research, edited by D.G. Land and H.E. Nursten, Applied Science, London, 1979, p. 79.

    Google Scholar 

  21. Grosch, W., Detection of Potent Odorants in Foods by Aroma Extract Dilution Analysis, Trends Food Sci. Technol. 4:68 (1993).

    Article  CAS  Google Scholar 

  22. Van den Dool, H., and P. Kratz, Generalization of the Retention Index System Including Linear Temperature Programmed Gas-Liquid Partition Chromatography, J. Chromatogr. 11:463 (1963).

    Article  Google Scholar 

  23. Wilcox, M.H., and B.L. Jones. Enterococci and Hospital Laundry, The Lancet 345:594 (1995).

    Article  CAS  Google Scholar 

  24. Gohl, E.P.G., and L.D. Vilensky, The Synthetic Fibres, in Textile Science, 2nd edn., Longman Cheshire, Melbourne, 1983, p. 89.

    Google Scholar 

  25. Zeng, X., J. Leyden, H.J. Lawley, D. Sawano, I. Nohara, and G. Preti, Analysis of Characteristic Odors from Human Male Axillae, J. Chem. Ecol. 17:1469 (1991).

    Article  CAS  Google Scholar 

  26. Zhu, J., and L.D. Jakobsen, Correlating Microbes to Major Odorous Compounds in Swine Manure, J. Environ. Qual. 28: 737 (1999).

    Article  CAS  Google Scholar 

  27. Dussault, F., C. Dube, J. Morris, E.J. Squires, and A. Houde, Microbial Growth on Porcine Meat Containing Different 16-Androstene Steroids Levels, Lebensm. Wiss. Technol.-Food Sci. Technol. 33:239 (2000).

    Article  CAS  Google Scholar 

  28. Moss, B.W., S.M. Hawe, and N. Walker, Sensory Thresholds for Skatole and Indole, in Colloques de l'INRA Measurement and Prevention of Boar Taint in Entire Male Pigs, INRA, Paris, 1993, p. 63.

    Google Scholar 

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Correspondence to Signe Munk.

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Munk, S., Johansen, C., Stahnke, L.H. et al. Microbial survival and odor in laundry. J Surfact Deterg 4, 385–394 (2001). https://doi.org/10.1007/s11743-001-0192-2

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  • DOI: https://doi.org/10.1007/s11743-001-0192-2

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