Quick start-up of EGSB reactor treating fresh leachate of municipal solid waste


An expanded granular sludge bed (EGSB) reactor inoculated with anaerobic granular sludge was started up with its COD removal performance, self-balancing of pH, biogas production rate and characteristics of the granular sludge during the start-up period being investigated. The results indicated that the EGSB reactor can be started up successfully in 27 d by increasing the organic loading rate rapidly. The removal efficiency of COD was maintained above 93% with influent COD concentration of 25 000 mg/L and OLR of 37.94 kgCOD/(m3·d). The EGSB reactor with good pH self-balancing could be fed with fresh leachate of low pH value (4–5). The biogas production rate was closely related with OLR and COD reduction. Every gram of COD fed (consumed) to the reactor produced 0.34 LSTP (0.36 LSTP) biogas with 0.21 LSTP (0.23 LSTP) methane. Thus, 65%–70% of the produced biogas was methane. Sixty one percentage of COD fed to the reactor was converted to methane, another 33% was converted to biomass by metabolism, and the other 6% was left in the liquid phase. The specific methanogenic activity (SMA) of the granular sludge had increased by 92% after 27 d dynamic incubation. The granular sludge in the reactor had good settlement performance with majority diameter of 1–2 mm.

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  1. [1]

    Liu Guo-qiang, Fang Fang, Guo Jin-song, Zhang Xiao-wei, Gong Lu. Seasonal variation of water quality of landfill and incineration leachates in Three Gorges Area [J]. Research of Environmental Sciences, 2008, 21(4): 43–47 (in Chinese).

    Google Scholar 

  2. [2]

    Punal A, Trevisan M, Rozzi A, Lema J M. Influence of C:N ratio on the start-up of upflow anaerobic filter reactors [J]. Water Research, 2000, 34(9): 2614–2619.

    Article  Google Scholar 

  3. [3]

    Zhou Jin, Wang Bao-quan, Yu Ban-chi. Granular activated carbon dosing UASB reactor to speed up the process of particle [J]. China Water and Wastewater, 1996, 12(5): 16–19 (in Chinese).

    MATH  Google Scholar 

  4. [4]

    Wang Jin-song, Hu Yong-you. Mechanism of anaerobic granulation enhancement with bio-flocculant [J]. Acta Scientiae Circumstantiae, 2005, 25(3): 361–366 (in Chinese).

    Google Scholar 

  5. [5]

    Hou Yong-you, Zeng Ke, Cai Fa-qi. Effects of different kinds of sludge on start-up of IC reactor in treatment of soybean protein wastewater [J]. IndustrialWater and Wastewater, 2007, 3(6): 41–45 (in Chinese).

    Google Scholar 

  6. [6]

    Connaughton S, Collins G, O’flaherty V. Psychrophilic and mesophilic anaerobic digestion of brewery effluent: A comparative study [J]. Water Research, 2006, 40(13): 2503–2510.

    Article  Google Scholar 

  7. [7]

    Li Jin, Zuo Jian-e, Xing Wei. Performance and microbial characteristics of an EGSB reactor treating brew ery wastewater under 20 °C [J]. Environmental Science, 2008, 29(4): 990–995 (in Chinese).

    Google Scholar 

  8. [8]

    Puyol D, Mohedano A F, Sanz J L, Rodriguez J J. Comparison of UASB and EGSB performance on the anaerobic biodegradation of 2,4-dichlorophenol [J]. Chemosphere, 2009, 76(9): 1192–1198.

    Article  Google Scholar 

  9. [9]

    Chen C, Wang A J, Ren NQ, Lee D J, Lai J Y. Highrate denitrifying sulfide removal process in expanded granular sludge bed reactor [J]. Bioresource Technology, 2009, 100(7): 2316–2319.

    Article  Google Scholar 

  10. [10]

    Yu H Q, Fang H H P, Tay J H. Enhanced sludge granulation in upflow anaerobic sludge blanket(UASB) reactors by aluminum chloride [J]. Chemosphere, 2001, 44(1): 31–36.

    Article  Google Scholar 

  11. [11]

    Timur H, Ozturk I. Anaerobic sequencing batch reactor treatment of landfill leachate [J]. Water Research, 1999, 33(15): 3225–3230.

    Article  Google Scholar 

  12. [12]

    Agdag O N, Sponza D T. Anaerobic/aerobic treatment of municipal landfill leachate in sequential two-stage up-flow anaerobic sludge blanket reactor (UASB)/completely stirred tank reactor (CSTR) systems [J]. Process Biochemistry, 2005, 40(2): 895–902.

    Article  Google Scholar 

  13. [13]

    Castillo E, Vergara M, Moreno Y. Landfill leachate treatment using a rotating biological contactor and an upward-flow anaerobic sludge bed reactor [J]. Waste Management, 2007, 27(5): 720–726.

    Article  Google Scholar 

  14. [14]

    Bohdziewicz J, Neczaj E, Kwarciak A. Landfill leachate treatment by means of anaerobic membrane bioreactor [J]. Desalination, 2008, 221(3): 559–565.

    Article  Google Scholar 

  15. [15]

    Laguna A, Ouattra A, Gonzalez R O, Baron O, Fama G, I Mamouni R, Guiot S, Monroy O, Macarie H. A simple and low cost technique for determining the granulometry of upflow anaerobic sludge blanket reactor sludge [J]. Water Science Technology, 1999, 40(8): 1–8.

    Article  Google Scholar 

  16. [16]

    He Yan-Ling. Anaerobic wastewater treatment [M]. Beijing: China Light Industry Press, 1999 (in Chinese).

    Google Scholar 

  17. [17]

    Tay J H, Tay S T L, Liu Y. Biogranulation technologies for wastewater treatment [M]. UK: Elsevier Publication, 2006.

    Google Scholar 

  18. [18]

    Liu Yong-hong, He Yan-ling, Li Yao-zhong, Yang Shucheng. The settling characteristics and terminal velocity of granular sludge in UASB reactor [J]. Acta Scientiae Circumstantiae, 2005, 25(2): 176–179 (in Chinese).

    Google Scholar 

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Correspondence to Jian-yong Liu 刘建勇.

Additional information

Project supported by the Science and Technology Commission of Shanghai Municipality (Grant Nos.10DZ120010C, D9DZ2251700)

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Liu, J., Bian, H., Cao, Y. et al. Quick start-up of EGSB reactor treating fresh leachate of municipal solid waste. J. Shanghai Univ.(Engl. Ed.) 15, 212–217 (2011). https://doi.org/10.1007/s11741-011-0723-3

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  • expanded granular sludge bed (EGSB) reactor
  • municipal solid waste
  • fresh leachate
  • start-up
  • biogas