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The effect of light on the level of acetylcholine in seedlings of the wild-type and phytochrome mutants of tomato (Lycopersicon esculentum Mill.)

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Abstract

Applying the method of pyrolysis coupled with gas chromatography (PYR-GC) the content of endogenous acetylcholine (ACh) was investigated in the extracts obtained from tomato (Lycopersicon esculentum Mill.). Seven-day-old seedlings of wild type (WT) and phytochrome mutants au (aurea), hp (high pigment), fri (far-red light insensitive) and tri (temporarily red light insensitive) were studied. In the analyzed material the presence of choline and acetylcholine was discovered. The highest content of ACh (381 mmole/g of fresh weight) was found in tomato cotyledons, whereas the lowest amount (162 nmole/g of fresh weight) in roots. The level of ACh in the plants grown under the continuous light was higher than in etiolated ones. However, no considerable differences in the concentrations of ACh in au and tri seedlings grown under the continuous light and in darkness were observed. The irradiation of etiolated seedlings of wild type with red light was accompanied by the increase of endogenous level of ACh. The pulse of far-red light applied directly after red light reversed this stimulating effect. A similar effect of both light wavelengths on the content of ACh was also found in the case of the tri mutant. On the other hand, in the case of fri mutant, pulse of red light caused the drop in the content of ACh, whereas far-red applied after red light caused visible increase in the level of the investigated substance. In tissues of au mutant no effect of red and far-red lights on the concentration of ACh was established.

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Abbreviations

Ach:

acetylcholine

AChE:

acetylcholinesterase

AChR:

acetylcholine receptor

ChAT:

choline acetyltransferase

FR:

far red light

PhyA:

labile phytochrome

PhyB:

stable phytochrome

PHYA :

gene of the labile phytochrome

PHYB :

gene of the stable phytochrome

R:

red light

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Authors and Affiliations

  1. Department of Plant Physiology and Morphogenesis, Nicholas Copernicus University, Institute of General and Molecular Biology, Gagarina 9, PL-87-100, Toruń, Poland

    Justyna Wiśniewska & Andrzej Tretyn

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  1. Justyna Wiśniewska
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  2. Andrzej Tretyn
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Wiśniewska, J., Tretyn, A. The effect of light on the level of acetylcholine in seedlings of the wild-type and phytochrome mutants of tomato (Lycopersicon esculentum Mill.). Acta Physiol Plant 21, 221–230 (1999). https://doi.org/10.1007/s11738-999-0036-6

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  • DOI: https://doi.org/10.1007/s11738-999-0036-6

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