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Exocellular (glyco)proteins of Lupinus albus plants and suspension-cultured cells

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Abstract

Plant species from genus Lupinus are among the oldest known legumes, and various aspects of their biology are considerably different from those commonly observed within Leguminosae. To study this issue in more detail, a suspension culture of Lupinus albus cells was developed, and the glycosylation patterns of exocellular proteins analysed. N-linked oligosaccharide side-chains were detected with two lectins: concanavalin A (ConA) and wheat germ agglutinin (WGA) used with respective anti-lectin antibodies, while O-linked arabinosylated side-chains of (hydroxy)proline-rich glycoproteins were identified with anti-(42 kDa French bean chitin-binding protein) antibodies. The obtained data were compared with analogous ones for exocellular (glyco)proteins from suspension-cultured Phaseolus vulgaris cells and from various tissues of L. albus plants. Major species-specific differences between exocellular (glyco)proteins from lupin and bean cells were identified. Similarly, developmentally regulated glycosylation changes following transition from organised plant tissue to dedifferentiated suspension-cultured lupin cells were detected and analysed.

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Abbreviations

BSA:

bovine serum albumin

ConA:

concanavalin A

ECM:

exocellular matrix

SDS-PAGE:

sodium dodecyl sulphatepolyacrylamide gel electrophoresis

TBST:

10 mM Tris/HCl (pH 8.0), 0.15 M NaCl, 0.05% (v/v) Tween 20

WGA:

wheat germ agglutinin

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznań, Poland

    Przemysław Wojtaszek & Mariola Piślewska

  2. Division of Biochemistry, School of Biological Sciences, Royal Holloway, University of London, TW20 0EX, Egham, Surrey, England

    G. Paul Bolwell

Authors
  1. Przemysław Wojtaszek
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  2. Mariola Piślewska
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  3. G. Paul Bolwell
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Przemysław, W., Piślewska, M. & Bolwell, G.P. Exocellular (glyco)proteins of Lupinus albus plants and suspension-cultured cells. Acta Physiol Plant 20, 299–305 (1998). https://doi.org/10.1007/s11738-998-0062-9

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  • DOI: https://doi.org/10.1007/s11738-998-0062-9

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