The influence of Bipolaris sorokiniana and Drechslera dictyoides metabolites on cell membrane permeability of Festuca pratensis callus
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The aim of this study was the evaluation of membrane permeability of callus cells of several Polish meadow fescue cultivars, which were treated with toxins of two leaf spot pathogens Bipolaris sorokiniana and Drechslera dictyoides. Fungus metabolites were obtained by the method described by Lepoivre et al. (1986). Calli of cultivars ‘Skrzeszowicka’, ‘Skawa’, ‘Westa’, POB 282, POB 383, KOA 186 have been selected on medium with metabolites for two weeks. Next the conductivity test of electrolyte leakage and of total ion contents in the examined tissue was done. On the base of this data the membrane permeability coefficients for each cultivar were calculated. Toxins of B. sorokiniana damaged the cell membranes more strongly than metabolites of D. dictyoides. The significant differences of several objects sensitivity to the influence of B. sorokiniana metabolites were stated. These differences were not observed in the case of the influence of D. dictyoides metabolites on the examined tissue.
Key wordsBipolaris sorokiniana callus conductivity test Drechslera dictyoides metabolites membrane permeability
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- Janowiak F., Markowski A. 1987. Effect of chilling on germination, growth, survival and membrane permeability in seedlings of different breeding forms of maize (Zea mays L.) Acta Physiol. Plant. 9: 77–87.Google Scholar
- Kauss H. 1990. Role of the plasma membrane in hostpathogen interactions. In: C. Larsoon, J.M. Møller (Eds). The plant plasma membrane. Springer-Verlag Berlin, Heidelberg: 321–350.Google Scholar
- Lepoivre P., Viseur J., Duhem K., Carels N. 1986. Double layer technique as a tool for the selection of calluses resistant to toxic material from plant pathogenic fungi. J. Semal (Ed) “Somaclonal variation and crop improvement”, Martinus Nijhoff Publ.: 45–53.Google Scholar
- Płażek A. 1992. The callus obtaining from ripe seeds of timothy (Phleum pratense L.) Biul. IHAR 183: 243–249. (In Polish)Google Scholar
- Płażek A. 1993. The resistance evaluation of meadow fescue callus (Festuca pratensis Huds.) to metabolites of Drechslera sp. fungi. Biul. IHAR 188: 43–50. (In Polish)Google Scholar
- Płażek A. 1994. The influence of metabolites secreted by Bipolaris sorokiniana (Sacc.) Shoem. on the development of callus of some meadow fescue cultivars and strains. Genetica Polonica 35 A: 101–104. (In Polish)Google Scholar
- Wakuliński W. 1989. Phytotoxicity of Fusarium metabolites in relation to pathogenicity. In: Fusarium-Mycotoxins, Taxonomy and Pathogenicity. Ed. Chełkowski J. Elsevier, Amsterdam, 257–268.Google Scholar
- Wojciechowski S., Chełkowski J., Kostecki M. 1995. Influence of deoxynivalenol on electrolyte leakage in cereal seedling leaves. Acta Physiol. Plant. 17: 357–360.Google Scholar
- Wojciechowski S., Wiśniewska H., Chełkowski J. 1996. Influence of Fusarium culmorum infection and its metabolite deoxynivalenol on membranes stability in barley seedlings. Acta Physiol. Plant. 18: 3–6.Google Scholar