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Effect of inhibitors of ethylene biosynthesis and action, as well as calcium and magnesium on rose shoot rooting, shoot-tip necrosis and leaf senescence in vitro

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Abstract

Microcuttings of easy-to-root dwarf rose cv. Starina, showing early symptoms of leaf senescence and shoot-tip necrosis in rooting stage, were chosen for the study. The effects of inhibitors of ethylene biosynthesis (AOA, AIB) and action (AgNO3), and Ca2+ and Mg2+ were studied in relation to rooting, leaf senescence and shoot-tip necrosis. The effects of these substances were examined with respect to IAA presence in a medium, which stimulated leaf yellowing and shoot-tip necrosis. AOA strongly inhibited rooting of microcuttings, but did not affect ethylene biosynthesis. AIB at 250 mg·l−1 and AgNO3 2.5 mg·l−1 in the presence of IAA did not affect rooting but effectively prevented leaf senescence. Ca2+ alone or combined with Mg2+ at raised concentration, or an ethylene action inhibitor Ag+, reduced shoot-tip necrosis in microcuttings treated with IAA. Addition of Ag+ to IAA medium drastically increased ethylene production by the shoots. Interaction between endogenous levels of auxin, ethylene and calcium in relation to rooting, shoot-tip necrosis and leaf senescence was discussed. Ethylene could enhance tissue sensitivity to auxin. Moreover, the tissue of rose shoots is very sensitive in the in vitro condition on standard medium because of the calcium deficiency. Thus, the raised Ca/Mg level counteracted shoot-tip necrosis through enhancing cell membrane and wall resistance to ethylene and IAA.

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Abbreviations

BA:

6-benzylaminopurine

GA3 :

gibberellic acid

IAA:

1H-indole-3-acetic acid

AOA:

aminooxyacetic acid

AIB:

1-aminoisobutyric acid

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  1. Research Institute of Pomology and Floriculture, 96-100 Skierniewice, Pomologiczna 18, Poland

    Małgorzata Podwyszyńska & Danuta M. Goszczyńska

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  1. Małgorzata Podwyszyńska
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  2. Danuta M. Goszczyńska
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Podwyszyńska, M., Goszczyńska, D.M. Effect of inhibitors of ethylene biosynthesis and action, as well as calcium and magnesium on rose shoot rooting, shoot-tip necrosis and leaf senescence in vitro . Acta Physiol Plant 20, 91–98 (1998). https://doi.org/10.1007/s11738-998-0049-6

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  • DOI: https://doi.org/10.1007/s11738-998-0049-6

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