Abstract
This paper is a continuation of our studies related to the response of two tomato cultivars: Robin and New Yorker to chilling: the later is more tolerant to chilling than the former one (Starck et al. 1994). The concentration of ABA in the xylem sap and ABA delivery rate (calculated as the amount of ABA exuded in 2h from the cut stump, following shoot removal) were estimated by ELISA. The relative water content (RWC) of the leaf blades and stomatal resistance (RS) were also measured. Tomato plants were grown in a greenhouse, under noncontrolled conditions. Before chilling some of the plants were drought hardened for 10 days (H). As an consequence of water deficit only New Yorker growth slightly decreased. Plants were chilled to 2–5 °C during three consecutive, 16-h nights, preceded by warm days, which caused a decrease in the RWC of leaf blades. Chilling did not decreased leaf blade hydration significantly, but drastically increased the concentration of ABA in the xylem sap in more chilling tolerant cv. New Yorker only. The delivery rate of ABA was markedly enhanced in both cultivars, but much more in New Yorker. Drought hardening increased ABA delivery rate in cv. Robin only, especially after chilling. The lack of correlation between changes in the RWC of leaf blades after low temperature treatment and the concentration of ABA in the xylem sap as well as its delivery rate suggest, that in both tomato cultivars chilling increased ABA level directly, not as an secondery effect of temperature-induced water deficit.
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References
Bohnert H.J., Nelson D.E., Jensen R.G. 1995. Adaptations to environmental stresses. The Plant Cell, 7: 1099–1111.
Bray E.A. 1990. Drought-stress-induced polypeptide accumulation in tomato leaves. Plant Cell Environ., 13: 531–538.
Bray E.A. 1997. Plant responses to water deficit. Trends in Plant Science, 2/2: 48–54.
Brüggeman W., van der Kooij T.A.W., Hasselt P.R. 1992. Long-term chilling of young tomato plants under low light and subsequent recovery. Planta, 186: 172–178.
Capell B., Dörffling K. 1993. Genotype-specific differences in chilling tolerance of maize in relation to chilling-induced changes in water status and abscisic acid accumulation. Physiol. Plant., 88: 638–646.
Choluj D., Kalaji H.M., Niemyska B. 1997. Analysis of gas exchange components in tomato plants. Photosynthetica, in press.
Correia M.J., Periera J.S., Chaves M.M., Rodrigues M.L., Pacheco C.A. 1995. ABA xylem concentrations determine maximum daily leaf conductance of fieldgrown Vitis vinifera L. plants. Plant Cell Environment, 18: 511–521.
Daie J., Campbell W.F. 1981. Response of tomato plants to stressfull temperatures. Plant Physiol., 67: 26–29.
Eamus D., Wilson J.M. 1983. ABA levels and effects in chilled and hardened Phaseolus vulgaris. J. Exp. Bot. 34/145: 1000–1006.
Else M.A., Davies W.J., Whitford P.N., Hall K.C., Jackson M.B. 1994. Concentrations of abscisic acid and other solutes in xylem sap from root systems of tomato and castor-oil plants are distorted by wounding and variable sap flow rates. J. Exp. Bot., 45: 317–323.
Grau A., Halloy S. 1997. Effect of chilling on CO2 gasexchange in Carica papaya L. and Carica quercifolia (A.St.Hil.) Solms. J. Plant Physiol., 150: 475–480.
Hartung W., Davies W.J. 1991. Drought-induced changes in physiology and ABA. 63–79. In: Abscisic acid: physiology and biochemistry. Eds. Davies W.J., Jones H.G. Bios Sci Publ.
Irigoyen J.J., Perez de Juan J., Sanchez-Diaz M. 1996. Drought enchances chilling tolerance in a chilling-sensitive maize (Zea mays) variety. New Phytol. 134: 53–59.
Janowiak F., Dörffling K. 1996. Chilling of maize seedlings: changes in water status and abscisic acid content in ten genotypes differing in chilling tolerance. J. Plant Physiol., 147: 582–588.
Jokhan A.D., Else M.A., Jackson B. 1996. Delivery rates of abscisic acid in xylem sap of Ricinus comunis L. plants subjected to part-drying of the soil. J.Exp.Bot., 47: 1595–1599.
Mäntylä E., Lang V., Palva E.T. 1995. Role of abscisic acid in drought-induced freezing tolerance, cold acclimation, and accumulation of LTI78 and RAB18 proteins in Arabidopsis thaliana. Plant Physiol., 107: 141–148.
Markhart A.H., III. 1986. Chilling injury: a review of possible causes. Hort Sci., 21/6: 1329–1333.
McKersie B.D., Leshem Y.Y. 1994. Stress and stress coping in cultivated plants. Kluwer Ac. Publ. Dordrecht, Boton, London.
Mertens R., Deus-Neuman B., Weiler E.W. 1983. Monoclonal antibodies for the detections and quantification of the endogenous plant growth regulator. FEBS letters. 160: 269–272.
Pardossi A., Vernieri P., Tognoni F. 1992. Involvement of abscisic acid in regulating water status in Phaseolus vulgaris L. during chilling. Plant Physiol., 100: 1243–1250.
Pekič S., Stikič R., Tomljanovič L., Andjelkovič V., Ivanovič M., Quarrie S.A. 1995. Characterization of maize lines differing in leaf abscisic acid content in the field. 1 Abscisic acid physiology. Ann. Bot., 75: 67–73.
Perez de Juan J., Irigoyen J.J., Sanchez-Diaz M. 1997. Chilling of drought-hardened and non-hardened plants of different chilling-sensitive maize lines. Changes in water relations and ABA contents. Plant Science 122: 71–79.
Schulze E.D. 1993. Soil water deficits and atmospheric humidity as environmental signals. In: Smith JA.C. & Griffiths H. (eds.) Water deficits-plant responses from cell to community, 129–131 Bios Scientific Publishers.
Smoleńska-Sym G., Gawrońska H., Kacperska A. 1995. Modifications of abscisic acid level in winter oil-seed rape leaves during acclimation of plants to freezing temperatures. Plant Growth Regul., 17: 61–65.
Starck Z., Choluj D., Kalaji H.M. 1994. Photosynthesis and biomass allocation as response to chilling in tomato plants. In: Dörffling K, Brettschneider B., Tantau H., Pithan K (ed): Adaptation To Cool Climates COST 81 Workshop. Pp. 125–132. European Commission. European Cooperation in the Field of Scientific and Technical Research, Brussels-Luxembourg.
Starck Z., Bogdan J., Choluj D. 1997. Effect of phosphorus and patassium supply on tomato plant response to chilling stress. Symp. Crop adaptation to cool climates, COST, in press.
Vernieri P., Pardossi A., Tognoni F. 1989. Chilling-induced water stress in tomato: effect on abscisic acid accumulation. Adv. Hort. Sci., 3: 78–80.
Vernieri P., Pardossi A., Tognoni F. 1991. Influence of chilling and drought on relations and abscisic acid accumulation in bean. Aust. J. Plant Physiol., 18: 25–35.
Vernieri P., Pardossi A., Serra G., Tognoni F. 1994. Changes in abscisic acid and its glucose ester in Phaseolus vulgaris L. during chilling and water stress. Plant Growth Regul., 15: 157–163.
Wilson J.M. 1976. The mechanism of chill- and drought- hardening of Phaseolus vulgaris leaves. New Phytol., 76: 257–270.
Zhang C-L., Li P.H., Brenner M.L. 1986. Relationship between mefluidide treatment and abscisic acid metabolism in chilled corn leaves. Plant Physiol., 81: 699–701.
Zhang J., Davies W.J. 1990a. Change in the concentration of ABA in xylem sap as a functions of changing soil water status can account for changes in leaf conductance and growth. Plant Cell Environ., 13: 277–285.
Zhang J., Davies W.J. 1990b. Does ABA in the xylem control the rate of leaf growth in soil-dried maize and sunflower plants? J. Exp. Bot., 41: 1125–1132.
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Starck, Z., Choluj, D. & Gawrońska, H. The effect of drought hardening and chilling on ABA content in xylem sap and ABA - delivery rate from root of tomato plant. Acta Physiol Plant 20, 41–48 (1998). https://doi.org/10.1007/s11738-998-0041-1
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DOI: https://doi.org/10.1007/s11738-998-0041-1