Abstract
Nodal segements were taken from juvenile shoots of mature 100 year-old trees of saucer magnolia (Magnolia x soulangiana Soul.-Bod.) and cultured on Standardi and Catalano medium supplemented with 1.33 µmol·dm−3 BA, 0.54 µmol·dm−3 NAA, 58 µmol·dm−3 sucrose and 6.0 g·l−1 agar-agar. After 8 weeks, separated shoots were transferred to rooting medium with half-strength macronutrients (basal medium) supplemented with 0.3% activated charcoal and one of carbohydrates: arabinose, cellulose, fructose, galactose, glucose, lactose, mannose, rhamnose, ribose, sorbose, sucrose or xylose at 20 g·dm−3 and 7.0 g·dm−3 agar-agar. After 13 weeks of culture, shoot number, fresh and dry weight of shoots and roots, total root length and number of roots/per shoot were recorded. Percentages of rooted shoots were calculated. Fructose, mannose and xylose were the most effective carbon source on shoot proliferation followed by sucrose. The rooting response was induced by cellulose and xylose. Arabinose, rhamnose and sorbose inhibited root formation. The number of adventitious roots produced per shoot was stimulated by cellulose and xylose. Total biomass (shoot plus roots) of the plantlets was the highest at fructose and cellulose.
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Abbreviations
- N6 :
-
Benzylaminopurine (BA)
- α:
-
naphthaleneacetic Acid (NAA)
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Kamenicka, A. Influence of selected carbohydrates on rhizogenesis of shoots saucer magnolia in vitro . Acta Physiol Plant 20, 425–429 (1998). https://doi.org/10.1007/s11738-998-0030-4
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DOI: https://doi.org/10.1007/s11738-998-0030-4