Effect of 1.10-phenanthroline, a photodynamic herbicide on the development and structure of maize chloroplasts

Abstract

Effect of low (5 mmol·dm⁻³) and high (10 or 20 mmol·dm⁻³) doses of 1.10-phenanthroline (Phe), a photodynamic herbicide, on the development of chloroplasts in etiolated and subsequently illuminated maize seedlings and on the structure of already developed chloroplasts of green maize seedlings was examined. Etiolated and then irradiated plants were resistant to 5 mmol·dm⁻³ of Phe with respect to morphology, however Phe caused inhibition of greening and of grana formation. Higher Phe concentrations followed by exposure to light caused not only total inhibition of greening but also dilation of thylakoids, swelling of chloroplasts, and finally total destruction of chloroplast structure. Application of Phe in the same concentrations to green plants revealed that they were resistant to low dose of Phe with respect to morphology and structure of chloroplasts, however 10 and 20 mmol·dm⁻³ Phe and illumination caused the loss of turgor of treated plants and other photooxidative damages seen at the ultrastructural level. We concluded that maize, as representant of monocotyledonous plants, is resistant to low (5 mmol·dm⁻³) Phe concentration. Higher (10 or 20 mmol·dm⁻³) concentrations, used to determine the site of damage and mode of action of Phe on the level of cell revealed that action of photodynamic herbicides is based on standard photoinhibition mechanism and also probably on their chelating properties.