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Changes in proline level in response to osmotic stress and exogenous amino acids in Raphanus sativus L. seedlings

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Abstract

The changes in endogenous proline levels of Raphanus sativus L. seedlings was monitored in presence of exogenous amino acids in normal and osmotically stressed seedlings. In unstressed seedlings, proline uptake was detected only at higher (1 mM) concentration of applied L-proline. however, proline uptake was promoted at all (1 µM to 1000 µM) concentrations of applied L-proline under osmotic stress conditions. Amongst other exogenous amino acids, L-leucine, L-glutamic acid, L-alanine, and L-histidine enhanced endogenous levels of proline, while exogenous hydorxyproline and γ-amino butyric acid reduced it.

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References

  • Aspinall D., Paleg L.G. 1981. Proline accumulation — Physiological aspects. In: The physiology and biochemistry of drought resistance in plants. (Paleg L.G. and Aspinall D. eds) 205–240, Academic Press, Australia.

    Google Scholar 

  • Bates L.S., Waldren R.P., Teare I.D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205–207.

    Article  CAS  Google Scholar 

  • Carbonera D., Iadorola P., Cella R. 1989. Effects of exogenous amino acids on the intercellular content of proline and other amino acids in Daucus carota cells. Plant Cell Reports, 8: 422–424.

    Article  CAS  Google Scholar 

  • Fedina I.S., Tsonev T., Guleva E.I. 1993. The effect of pretreatment with proline on the responses of Pisum sativum to salt stress. Photosynthetica, 29: 521–527.

    CAS  Google Scholar 

  • Good A.G., Zaplachinski S.T. 1994. The effects of drought stress on free amino acid accumulation and protein synthesis in Brassica napus. Physiol. Plant. 90: 9–14.

    Article  CAS  Google Scholar 

  • Goring H., Thein B.H. 1979. Influence of nutrient deficiency on proline accumulation in cytoplasm of Zea mays seedlings. Biochem. Physiol. Pflanzen. 174: 9–16.

    Google Scholar 

  • Handa S., Handa A.K., Hasegava P., Bressan R.A. 1986. Proline accumulation and the adaptation of cultured plant cells in water stress. Plant Physiol. 80: 938–945.

    PubMed  CAS  Google Scholar 

  • Kumar V., Sharma D.R., Sheoran I.S. 1990. Effect of proline on growth, ionic content and osmotic potential of thioproline stressed and non stressed wild type callus cultures of mungbean (Vigna radiata). Ind. Jour. of Exptl. Biol, 28: 661–664.

    CAS  Google Scholar 

  • Oaks A., Mitchell D.J., Bernard R.A. Johanson F.J. 1970. The regulation of proline biosynthesis in maize roots. Canadian J. Bot. 48: 2249–2258.

    Article  CAS  Google Scholar 

  • Perez-Alfocea F., Estan M.T., Caro M., Guerrier G. 1993. Osmotic adjustment in Lycopersicum esculentum and Lycopersicum pennellii under sodium chloride and polyethylene glycol 6000 iso-osmotic stress. Physiol. Plant. 87: 493–498.

    Article  Google Scholar 

  • Raggi V. 1994. Changes in free amino acids and osmotic adjustment in leaves of water stressed bean. Physiol. Plant. 91: 427–434.

    Article  CAS  Google Scholar 

  • Rai V.K., Sharma U.D. 1991. Amino acids can modulate ABA induced stomatal closure, stomatal resistance and K+ fluxes in Vicia faba leaves. Beitrag. Biol. Pflanzen. 66:393–405.

    Google Scholar 

  • Rajgopal V. 1981. The influence of exogenous proline on the stomatal resistance in Vicia faba. Physiol. Plant. 52: 292–296.

    Article  Google Scholar 

  • Ranieri A., Bernardi R., Lanese P., Soldatini G.F. 1989. Changes in free amino acids content and protein pattern of maize seedlings under water stress. Environ. Exp. Bot. 29: 351–357.

    Article  CAS  Google Scholar 

  • Sharma U.D., Rai V.K. 1989. Modulation of osmotic closure of stomata, stomatal resistance and K+ fluxes by exogenous amino acids in Vicia faba leaves. Biochem. Physiol. Pflanzezn. 185: 369–376.

    CAS  Google Scholar 

  • Stewart C.R., Boggess S.F., Aspinall D., Paleg L.G. 1977. Inhibition of proline oxidase by water stress. Plant Physiol. 59: 930–932.

    PubMed  CAS  Google Scholar 

  • Thakur P.S., Rai V.K. 1982. Dynamics of amino acid accumulation of two differentially drought resistant Zea mays cultivars in response to osmotic stress. Environ. Exp. Bot. 22: 221–226.

    Article  CAS  Google Scholar 

  • Thakur P.S., Rai V.K. 1985. Exogenously supplied amino acids and water deficits in Zea mays cultivars. Biol. Plantarum. 27: 458–461.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biosciences, Himachal Pradesh university, 171005, Shimla, India

    Shibha Khanna & V. K. Rai

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  1. Shibha Khanna
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  2. V. K. Rai
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Khanna, S., Rai, V.K. Changes in proline level in response to osmotic stress and exogenous amino acids in Raphanus sativus L. seedlings. Acta Physiol Plant 20, 393–397 (1998). https://doi.org/10.1007/s11738-998-0025-1

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  • DOI: https://doi.org/10.1007/s11738-998-0025-1

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