Skip to main content
SpringerLink
Log in

Effect of plant growth regulators and different nitrogen sources on NADP-isocitrate dehydrogenase activity of radish cotyledons

  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

NADP-isocitrate dehydrogenase (NADP-ICDH) activity of radish (Raphanus sativus L.) seedlings pretreated with various plant growth regulators: KiN, GA, and ABA and different nitrogen sources viz. KNO3, NH4Cl and NH4NO3 in light and dark was investigated. ICDH activity was significantly higher in light than in dark; addition of different nitrogen sources reduced it to a greater extent in NO3 supplementation. Among hormonal treatment only KiN showed slight promotion with KNO3 and NH4NO3. On the other hand in light KNO3 and/or NH4NO3 promoted ICDH activity and among hormones, KiN significantly promoted the activity in KNO3 and NH4NO3 supplemented seedlings while ABA was effective in NH4CL. It is suggested that in non-photosynthetic tissues, NADP-ICDH provides both reductant and carbon skeleton for glutamate synthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ICDH:

isocitrate dehydrogenase

KiN:

kinetin

GA:

gibberellic acid

ABA:

abscisic acid

DW:

distilled water

References

  • Attucci S., Rivoal J., Brouquisse R., Carde J.P., Pradet A. and Raymond P. 1994. Characterization of a mitochondrial NADP-dependent isocitrate dehydrogenase in axes of germinating sunflower seeds. Plant Sci. 102:49–59.

    Article  CAS  Google Scholar 

  • Chanda S.V. and Singh Y.D. 1996. Biochemical analysis of developing wheat grains. J. Agron. Crop Sci. 176:131–139.

    Article  CAS  Google Scholar 

  • Chen R.D. and Gadal P. 1990. Do the mitochondria provide the 2-oxoglutarate needed for glutamate synthesis in higher plant chloroplasts? Plant Physiol. Biochem. 28:141–145.

    CAS  Google Scholar 

  • Chen R.D., Le Marechal P., Vidal J., Jacquot J.P. and Gadal P. 1988. Purification and comparative properties of the cytosolic isocitrate dehydrogenase (NADP) from pea (Pisum sativum) roots and green leaves. Eur. J. Biochem. 175:565–572.

    Article  PubMed  CAS  Google Scholar 

  • Chen R.D., Bismuth E., Champigny M.L. and Gadal P. 1989. Chromatographic and immunological evidence that chloroplastic and cytosolic pea (Pisum sativum L.) NADP-isocitrate dehydrogenases are distinct isoenzymes. Planta 178:153–163.

    Article  Google Scholar 

  • Doddema H. and Telkamp G.P. 1979. Uptake of nitrate by mutants of Arabidopsis thaliana, disturbed in uptake or reduction of nitrate II kinetics. Physiol. Plant. 45:332–338.

    Article  CAS  Google Scholar 

  • Fieuw S., Muller-Robert B., Galvez S. and Willmitzer L. 1995. Cloning and expression analysis of the cytosolic NADP+-dependent isocitrate dehydrogenase from potato. Plant Physiol. 107:905–913.

    Article  PubMed  CAS  Google Scholar 

  • Hanning I. and Heldt H.W. 1993. On the function of mitochondrial metabolism during photosynthesis in spinach (Spinacia oleracea) leaves: Partitioning between respiration and export of redox equivalents and precursor for nitrate assimilation products. Plant Physiol. 103:1147–1154.

    PubMed  CAS  Google Scholar 

  • Joy K.W. 1988. Ammonia, glutamine and asparagine: a carbon-nitrogen interface. Can. J. Bot. 66:2103–2109.

    CAS  Google Scholar 

  • Miflin B.J. and Lea P.J. 1980. Ammonia assimilation. In The Biochemistry of Plants A Comprehensive Treatise Vol.5 Amino Acids and Derivatives Stumpf P.K. and Conn E.E. eds. Academic Press, New York, 169–202.

    Google Scholar 

  • Osmani S.A. and Scrutton M.C. 1985. The subcellular localization and regulation properties of pyruvate carboxylase from Rhizopus arrhizus. Eur. J. Biochem. 147: 119–128.

    Article  PubMed  CAS  Google Scholar 

  • Preiss J., Robinson N., Spilatro S. and Ncnamara K. 1985. Starch biosynthesis and its regulation. In Regulation of carbon partitioning in photosynthetic tissue. Heldt R.L. and Preiss J. eds. Waverly Press Baltimore M.D. 140–172.

  • Turpin D.H., Elrifi I.R., Birch D.G., Weger H.G. and Holmes J.J. 1988. Interactions between photosynthesis, respiration and nitrogen assimilation in microalgae. Can. J. Bot. 66:2083–2097.

    CAS  Google Scholar 

  • Turpin D.H., Vanlerberghe G.C., Amory A.M. and Guy R.D. 1991. The inorganic carbon requirements for nitrogen assimilation. Can. J. Bot. 69:1139–1145.

    CAS  Google Scholar 

  • Weger H.H. and Turpin D.H. 1989. Mitochondrial respiration can support NO3 and NO2/− reduction during photosynthesis:Interactions between photosynthesis, respiration and N assimilation in the N-limited green algae Selenastrum minutum. Plant Physiol. 89:409–415.

    Article  PubMed  CAS  Google Scholar 

  • Woo K.C., Boyle F.A., Flugge U.T. and Heldt H.W. 1987. 15N-ammonia assimilation, 2-oxoglutarate transport, and glutamate export in spinach chloroplasts in the presence of decarboxylates in the light. Plant Physiol. 85:621–625.

    PubMed  CAS  Google Scholar 

  • Yamazaki R.K. and Tolbert N. 1970. Enzymatic characterization of leaf peroxisomes. J. Biol. Chem. 245:5137–5144.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biosciences, Saurashtra University, 360 005, Rajkot, India

    C. R. Sood, S. V. Chanda & Y. D. Singh

Authors
  1. C. R. Sood
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. V. Chanda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. D. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sood, C.R., Chanda, S.V. & Singh, Y.D. Effect of plant growth regulators and different nitrogen sources on NADP-isocitrate dehydrogenase activity of radish cotyledons. Acta Physiol Plant 20, 353–357 (1998). https://doi.org/10.1007/s11738-998-0019-z

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-998-0019-z

Key words

Search

Navigation