Abstract
The accumulation of pyranocoumarins, the main active ingredient of Peucedanum praeruptorum Dunn, is strictly regulated by key enzyme genes in the coumarin biosynthesis pathway. However, little is understood about the correlation between the expression of key enzyme genes and the accumulation of coumarin content at various growth and development phases. In this work, a high-performance liquid chromatographic method and the real-time quantitative PCR system were prepared to establish the pyranocoumarins contents and the key enzyme expression in the roots, stems, and leaves of Peucedanum praeruptorum Dunn. The results showed that the coumarin content in the roots was the highest before blotting and was the lowest after flowering. Particularly, the content of Praeruptorin A, Praeruptorin B and Praeruptorin E was reduced to the lowest in roots after flowering. Consistent with the variation trend of the coumarin content in the stem, the relative expression of most key enzyme genes in the coumarin synthesis pathway was also significantly decreased. Especially for PpBMT gene, the relative expression in roots were extremely significantly decreased up to 90%. However, the variation trend of the coumarin content and the key enzyme genes expression was opposite in the roots and leaves. These results contribute to our knowledge of the process by which important enzymes control the production and accumulation of coumarin.
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Data availability
The data are available from the corresponding author on reasonable request.
Abbreviations
- BB:
-
Before bolting
- AB:
-
After bolting
- AF:
-
After flowering
- PAL:
-
Phenylalanine ammonia-lyase
- 4CL:
-
4-Coumarate: CoA ligase
- C4H:
-
Cinnamate 4-hydroxylase
- C2’ H:
-
P-coumaroyl CoA 2’-hydroxylase
- BMT:
-
Bergaptol O-methyltransferase
- COMT-S:
-
Caffeic acid O-methyltransferase
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Acknowledgements
We also thank Xiangwen Song from west Anhui University in China for helping with the test samples collection.
Funding
This work was supported by the excellent youth research projects in universities of the Anhui Province (2022AH030147), the High-level Talent Project of West Anhui University (No. WGKQ202001010), Anhui Provincial Science Fund for Distinguished Young Scholars (No. 1808085J17), and China Agriculture Research System (No. CARS-21).
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11738_2023_3619_MOESM1_ESM.pdf
The contents variation of peucedanocoumarin I, and peucedanocoumarin II in Peucedanum praeruptorum Dunn at different growth and development stages (BB: Before Bolting, AB: After Bolting, AF: After Flowering). (PDF 5381 kb)
11738_2023_3619_MOESM2_ESM.jpg
Fig S2. Proposed biosynthetic pathway of coumarins in Peucedanum praeruptorum Dunn. Dashed arrows represent multistep reactions and the ellipse represents the key enzymes in the coumarin biosynthesis pathway. PAL: phenylalanine ammonialyase, 4CL: 4-coumarate: CoA ligase, C4H: cinnamic acid 4-hydroxylase, C3′H cinnamoylester 3′-hydroxylase, C2‘H: p-coumaroyl CoA 2′-hydroxylase, HCThydroxycinnamoyl transferase, COMT: caffeoyl CoA O-methyltransferase, F6′H: Feruloyl CoA6′-hydroxylase, P5M: Psoralen 5-monoooxgenase, OMT: O-methyl-transferase, BMT: Bergaptol O-methyltransferase (JPG 48 kb)
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Wei, P., Li, Y., Song, C. et al. Analysis of coumarin content and key enzyme genes expression involved in coumarin biosynthesis from Peucedanum praeruptorum Dunn at different stages. Acta Physiol Plant 45, 141 (2023). https://doi.org/10.1007/s11738-023-03619-3
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DOI: https://doi.org/10.1007/s11738-023-03619-3