Abstract
Ageratum conyzoides L. (Asteraceae) is a weed which is commonly distributed in all types of land. This weed plant is known to have antiinflammatory, antimicrobial, antiasthmatic properties and essential oil with insecticidal, antiprotozoan, antibacterial, and antioxidant activities. The main objective of this study is to increase the production of Precocene II in the leaf derived root culture of A. conyzoides elicited with 100 µM methyl jasmonate (MeJA). Out of three auxins (IBA, IAA, and NAA) tested for root induction from leaf explant, MS medium fortified with 4.9 µM IBA favored better root formation (15.06 ± 1.27) after 3 weeks of culture. High-Performance Liquid Chromatography (HPLC) analysis of in vitro roots developed on 4.9 µM IBA was elicited with 100 µM MeJA resulted in the four fold increased production of bioactive compound, Precocene II than nonelicited roots. Precocene II showed better in vitro antioxidant activity against various test systems under in vitro. In addition, Precocene II showed better cytotoxic and apoptosis inducing potential against cervical cancer cell line (HeLa). Both antioxidant and cytotoxic activities of Precocene II were higher than elicited and nonelicited roots. Based on the results, it is understand that MeJA elicitation would be the good choice for increased production of biologically important compound, Precocene II from the root cultures of A. conyzoides. Moreover, the weed plant may be considered as a real source of valuable phytocompound.
References
Adebayo AH, Zeng GZ, Zhang YM, Ji CJ, Akindahunsi AA, Tan NH (2010) Toxicological evaluation of Precocene II isolated from Ageratum conyzoides L. (Asteraceae) in Sprague Dawley rats. Afr J Biotechnol 9:2938–2944
Ali M, Abbasi BH, Ali GS (2015) Elicitation of antioxidant secondary metabolites with jasmonates and gibberellic acid in cell suspension cultures of Artemisia absinthium L. PCTOC 120:1099–1106
Baque MA, Hahn EJ, Paek KY (2010) Induction mechanism of adventitious root from leaf explants of Morinda citrifolia as affected by auxin and light quality. In Vitro Cell Dev Biol Plant 46:71–80
Barros FMC, Almeida PC, Scopel RATE, Lucas AM, Bordignon SAL, Cassel E, Rubem MF, Vargas Poser G (2015) Chromenes from Ageratum conyzoides: Steam distillation, supercritical extraction, and mathematical modelling. Sep Sci Technol 51:307–315
Brana C, Benham C, Sundstrom L (2002) A method for characterizing cell death in vitro by combining propidium iodide staining with immunohistochemistry. Brain Res Protoc 10:109–114
Choi B, Kang S, Ha Y, Park G, Ackman RG (2002) Conjugated linoleic acid as a supplemental nutrient for common carp (Cyprinus carpio). Food Sci Biotechnol 11:457–461
Darzynkiewicz Z, Li X, Gong JP (1994) Assays of cell viability: Discrimination of cells dying by apoptosis. Methods Cell Biol 41:15–38
Dinis TC, Maderia VM, Almeida LM (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315:161–169
Ghoneim K, Basiouny A (2017) Insecticidal and anti-juvenile hormone activities of Precocene II against the Grasshopper Euprepocnemis plorans plorans (Charp.) (Orthoptera: Acrididae). IJTSRD 1:510–524
Halliwell B, Gutteridge JM, Aruoma OI (1987) The deoxyribose method: A simple “test-tube” assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 165:215–219
Ho TT, Murthy HN, Park SY (2020) Methyl jasmonate induced oxidative stress and accumulation of secondary metabolites in plant cell and organ cultures. Int J Mol Sci 21:716
Largia MJV, Pothiraj G, Shilpha J, Ramesh M (2015) Methyl jasmonate and salicylic acid synergism enhances bacoside A content in shoot cultures of Bacopa monnieri (L.). PCTOC 122:9–20
Lau CB, Ho CY, Kim CF, Leung KN, Fung KP, Tse TF (2004) Cytotoxic activities of Coriolus versicolor (Yunzhi) extract on human leukemia and lymphoma cells by induction of apoptosis. Life Sci 75:797–808
Ling APK, Tan KP, Hussein S (2013) Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata. J Zhejiang Univ Sci B 14:621–631
Mensor LL, Menezes FS, Leitao GG, Reis AS, dos Santos TC, Coube CS, Leitao SG (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Rese 15:127–130
Moreira MD, Picanco MC, Barbosa LCDA, Guedes RNC, Campos MRD, Silva GA, Martins JC (2007) Plant compounds insecticide activity against Coleoptera pests of stored products. Pesq Agropec Bras Brasilia 42:909–915
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Pratt GE, Bowers WS (1977) Precocene II inhibits juvenile hormone biosynthesis by cockroach corpora allata in vitro. Nature 265:548–550
Rajan M, Feba VC, Shahena, and Linu Mathew, (2020) Enhancement of rhamnetin production in Vernonia anthelmintica (L.) Willd. cell suspension cultures by eliciting with methyl jasmonate and salicylic acid. Physiol Mol Biol Plants 26:1531–1539
Vasantharani S, Thirugnanasampandan R, Bhuvaneswari G (2023) Qualitative and quantitative analysis of Precocene II, estimation of enzymatic, nonenzymatic antioxidant, and cytotoxic potentials of methyl jasmonate-elicited shoot culture of Ageratum conyzoides Linn. J Appl Biol 11:93–99
Xiao Nan Lu, Liu XC, Liu QZ, Liu ZL (2014) Isolation of insecticidal constituents from the essential oil of Ageratum houstonianum Mill. against Liposcelis bostrychophila Badonnel. J Chem 2014:645687
Yadav N, Ganie SA, Singh B, Chhillar AK, Yadav SS (2019) Phytochemical constituents and ethnopharmacological properties of Ageratum conyzoides L. Phytother Res 33:2163–2178
Zhang B, Chen L, Huo Y, Zhang J, Zhu C, Zhang X, Ma Z (2020) Establishment of adventitious root cultures from leaf explants of Tripterygium wilfordii (thunder god vine) for the production of celastrol. Ind Crops Prod 155:112834
Zhao GR, Xiang ZJ, Ye TX, Yuan YJ, Guo ZX (2006) Antioxidant activities of Salvia miltiorrhiza and Panaxnoto ginseng. Food Chem 99:767–774
Acknowledgements
The authors are thankful to the management of Kongunadu Arts and Science College (Autonomous) for providing necessary lab facility to carry out this work. The authors express our thanks to Sugarcane Breeding Institute (SBI), Coimbatore, Tamil Nadu, India for the HPLC analysis.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
We declare that there is no conflict of interest.
Additional information
Communicated by F. Araniti.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Vasantharani, S., Thirugnanasampandan, R. Methyl jasmonate elicitation on increased production of Precocene II from adventitious root cultures of Ageratum conyzoides L. and evaluation of its antioxidant and cytotoxic activities. Acta Physiol Plant 45, 125 (2023). https://doi.org/10.1007/s11738-023-03607-7
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11738-023-03607-7