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Genome-wide analysis and expression pattern of the PIN gene family during Korla fragrant pear calyx development

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Abstract

Because of genetic characteristics, sepals in Korla fragrant pear may remain on the fruit, the quality of “abscission calyx” fruit is better than that of “persistent calyx” fruit. In this study, the changes of hormone contents and auxin transporter PIN family-related gene expression during calyx decalyzation of Korla fragrant pear were studied. The key genes involved in auxin transport during abscission of the calyx tube were preliminarily screened. Bioinformatics analysis, HPLC and QRT-PCR were used to analyze the pear PIN gene family, IAA content determination and expression analyses PIN genes in young fruits. 15 PIN genes were screened and identified from the pear gene bank and named as PbPINs. They were divided into five subgroups and distributed unevenly on 11 chromosomes which contained 1–13 exons, 0–12 introns, 4–18 conserved motifs. PbPINs were mainly selected by purification during evolution. In this study, the change trends of the endogenous IAA contents at the 1st and 4th inflorescences were similar. The source IAA content decreased and changed on the 9th day after the large bud stage, and the content was low, and then the maximum value appeared on the 12th day. These indicated that the calyx-abscission rate of the young fruit reached a peak on the 9th day after the bud stage. Furthermore, PbPIN02, PbPIN05, PbPIN09, PbPIN10, PbPIN11, PbPIN12 and PbPIN14 were expressed at the 1st and 4th order flowers or young fruit calyx tubes, indicating that these genes played a certain role in auxin transport during calyx abscission. The expression levels of PbPIN02, PbPIN09 and PbPIN11 showed similar changes and were significantly higher than those of other genes, indicating that PbPIN02, PbPIN09 and PbPIN11 may play a greater role in auxin transport during calyx tube abscission of Korla fragrant pear.

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Abbreviations

cDNA:

Complementary DNA

CDSs:

Coding domain sequences

EDTA:

Ethylene diamine tetraacetic acid

GDR:

Genome database for Rosaceae

GSDS:

Gene structure display server

HMM:

Hidden Markov Model

HPLC:

High-performance liquid chromatography

IAA:

Indole-3-acetic acid

PIN:

PIN-FORMED

PGDD:

Plant genome duplication database

qRT-PCR:

Quantitative real-time polymerase chain reaction

WGD:

Whole-genome duplication

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Funding

This work was financially supported by the National Natural Science Foundation of China (31860528 and U2003121), Major Science and Technology Project of Corps (2017DB006), Bingtuan Science and Technology Program (2021CB055).

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Authors and Affiliations

  1. College of Plant Science, Tarim University, Alar City, China

    Min Jin, Qiang Zhang, Yan Chen, Hongyu Ma, Tianzheng Zhang, Cuiyun Wu, Rui Zhang, Qi Zhang & Jianping Bao

  2. National and Local Joint Engineering Laboratory of High-Efficient and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South Xinjiang, Alar City, China

    Min Jin & Jianping Bao

  3. College of Horticulture, Nanjing Agricultural University, Nanjing, China

    Xin Gong & Shutian Tao

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  1. Min Jin
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  2. Xin Gong
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  3. Qiang Zhang
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  4. Yan Chen
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  5. Hongyu Ma
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  9. Qi Zhang
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  10. Shutian Tao
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  11. Jianping Bao
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Correspondence to Jianping Bao.

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Communicated by M. Stobiecki.

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Jin, M., Gong, X., Zhang, Q. et al. Genome-wide analysis and expression pattern of the PIN gene family during Korla fragrant pear calyx development. Acta Physiol Plant 44, 55 (2022). https://doi.org/10.1007/s11738-022-03383-w

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  • DOI: https://doi.org/10.1007/s11738-022-03383-w

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