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Induction of hydrolytic enzyme activities in dormant seeds of Dracocephalum kotschyi Boiss. causes improvement of germination and seedling vigor indices

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Abstract

To overcome the seed dormancy of Dracocephalum kotschyi Boiss., a high-valuable herbaceous and medicinal plant, priming technique was applied using mechanical treatment (scarification, Scr) alone, or in combination with various chemical treatments under two groups viz. group-1: KNO3, KH2PO4, Na2SiO3, and group-2: ethanol, ascorbate, gibberellin, polyethyleneglycol (PEG), and complete Johnson nutrient solution (J.N.S). Results showed that scarification increases α-amylase, β-amylase, dehydrogenase, and protease enzymes activities and consequently causes higher seed germination compared to the control. Notably, significant positive effects were observed when the joint mechanical scarification–chemical-priming was applied. Enzymes’ activities and metabolite contents were increased in collective primed seeds, especially at the shorter priming times of group-1 and in Scr-GA3-Cold and SCr-J.N.S treatments of group-2. About 97–100% final germination percentage (FGP) and the first germination on 2nd–3rd day were obtained by Scr-GA3-Cold and SCr-J.N.S followed by the improvement of seedling growth indices. The highest GRI, TI, Vig (I), Vig (II), chlorophylls (Chl), the least mean germination time (MGT), and T50 indices were obtained by Scr-GA3-Cold treatment. In general, the most effective treatments for germination of seed and seedling growth improvement could be sorted as; Scr-GA3-Cold ≥ Scr-J.N.S > Scr-Ethanol, Scr-KNO3-(3h) > Scr-Asc ≥ Scr-PEG. The joint scarification + chemical-priming induced hydrolytic enzyme activities were more effective than other treatments and caused the breakdown of D. kotschyi seeds dormancy. Notably, hydrolytic enzyme activities (especially amylases) could be considered an indicator for selecting the best priming treatments in advance before seed planting.

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Acknowledgements

The authors thank the research council of the Lorestan University for Financial Support.

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Authors and Affiliations

  1. Department of Biology, Plant Physiology, Faculty of Science, Lorestan University, Khoramabad-Tehran Road (5th K), Iran

    Rezvan Ghannad & Maryam Madadkar Haghjou

  2. Key Lab of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Wuhan, 430062, China

    Ali Raza

  3. Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Mirza Hasanuzzaman

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  1. Rezvan Ghannad
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Correspondence to Maryam Madadkar Haghjou or Mirza Hasanuzzaman.

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There is no conflict of interest. Authors declare that using of endangered medicinal plant (Dracocephalum kotschyi Boiss.) is only for research aims and not for business/commercial goals, which was informed and was with the permission of the research council of University.

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Ghannad, R., Haghjou, M.M., Raza, A. et al. Induction of hydrolytic enzyme activities in dormant seeds of Dracocephalum kotschyi Boiss. causes improvement of germination and seedling vigor indices. Acta Physiol Plant 44, 48 (2022). https://doi.org/10.1007/s11738-022-03381-y

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