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Influence of nitrogen supply on gas exchange, chlorophyll fluorescence and grain yield of breeding lines of common bean evaluated in the Amazon region of Colombia

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Abstract

The common bean (Phaseolus vulgaris L.) is a food crop that provides fiber, vitamins and carbohydrates and it is produced by smallholders in developing countries under nitrogen (N)-depleted and low-fertility soil conditions. Soil acidity and the associated aluminum toxic and phosphorus deficient conditions are known to reduce symbiotic N-fixing ability of common bean. Breeding lines of common bean have been developed to adapt to acid-soil and high-temperature conditions in the Amazon region but the response of these lines to N application was not determined. The present study was conducted with an objective to identify genotypic differences among ten common bean breeding lines (4 Andean, 5 Mesoamerican, 1 interspecific) in their physiological characteristics (gas exchange, chlorophyll fluorescence and photosynthetic efficiency) and their contribution to superior agronomic performance under three different levels of N (0, 60 and 120 kg N ha−1; N0, N60 and N120) supply. We tested the hypothesis that common bean genotypes that combine greater capacity to assimilate carbon with improved ability to mobilize photosynthates to developing pods and seeds could enhance their agronomic performance at different levels of N supply under high-temperature and acid soil-stress conditions in the field. A randomized complete block design with factorial arrangement (3 levels of N × 10 genotypes) in three replications was used for the study. Measurements included photosynthetic gas exchange characteristics (light and CO2-saturated photosynthesis), chlorophyll fluorescence, viability of pollen, leaf N concentration, photosynthetic N use efficiency and grain yield. The increase in supply of N had a significant effect on grain yield and on different photosynthetic characteristics. Results showed that lines with indeterminate growth habit responded better to increase in N supply through their physiological response in improving grain yield. Among the genotypes evaluated, two lines (BFS 10 and SEF 10) were outstanding in their agronomic performance due to greater photosynthetic capacity combined with greater ability to mobilize photosynthates to grain with increase in N supply. These two lines can serve as parents for further improvement in resource use efficiency and multiple-stress resistance of common bean in the tropics.

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Acknowledgements

We acknowledge the financial support from the CGIAR Research Program on Grain Legumes and Dryland Cereals for the development of breeding lines of common bean. We would also like to thank all donors who supported this work through their contributions to the CGIAR Fund.

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Author notes

  1. Idupulapati M. Rao

    Present address: Plant Polymer Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 North University Street, Peoria, IL, 61604, USA

Authors and Affiliations

  1. Programa de Ingeniería Agroecológica, Facultad de Ingeniería, Universidad de la Amazonia, Florencia, Caquetá, Colombia

    Juan Carlos Suárez, José Alexander Anzola, Amara Tatiana Contreras, Dina Luz Méndez, José Iván Vanegas & Jhon Eduar Noriega

  2. Programa de Maestria en Sistemas Sostenibles de Producción, Facultad de Ingeniería, Universidad de la Amazonia, Florencia, Caquetá, Colombia

    Juan Carlos Suárez, José Alexander Anzola, Amara Tatiana Contreras, Dina Luz Méndez, José Iván Vanegas, Jhon Eduar Noriega & Leonardo Rodríguez

  3. Centro de Investigaciónes Amazónicas CIMAZ Macagual César Augusto Estrada González, Grupo de Investigaciones Agroecosistemas y Conservación en Bosques Amazónicos-GAIA, Florencia, Caquetá, Colombia

    Juan Carlos Suárez & Leonardo Rodríguez

  4. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, 62210, Cuernavaca, Mexico

    José A. Polanía

  5. The Alliance of Bioversity International and CIAT (Centro Internacional de Agricultura Tropical), The Americas Hub, Km 17 Recta Cali-Palmira, A.A. 6713, 763537, Cali, Colombia

    Milan O. Urban, Steve Beebe & Idupulapati M. Rao

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  1. Juan Carlos Suárez
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  2. José A. Polanía
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  11. Idupulapati M. Rao
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Correspondence to Juan Carlos Suárez.

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Suárez, J.C., Polanía, J.A., Anzola, J.A. et al. Influence of nitrogen supply on gas exchange, chlorophyll fluorescence and grain yield of breeding lines of common bean evaluated in the Amazon region of Colombia. Acta Physiol Plant 43, 66 (2021). https://doi.org/10.1007/s11738-021-03233-1

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