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Expression profile of genes involved in ramie flavonoids biosynthesis pathway and regulation of flavanone 3-hydroxylase (BnF3H) in response to aquatic environment

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Abstract

Flavonoids contained in ramie (Boehmeria nivea) root are important resources for officinal use. The plant seedling can grow optimally and develop an easy-harvesting adventitious root system in a hydroponic system. This research was carried out to unravel the regulation mechanism of flavonoid biosynthesis under hydroponic culturing condition, which hitherto remained unknown. Root samples of the ramie plants grown in soil and hydroponic conditions at different developmental stages were collected and pooled for transcriptome sequencing, and the expression levels of flavonoid biosynthesis-related genes and total flavonoid content were quantified. A total of 43,541 unigenes were finally annotated and the expression of 9 key unigenes were confirmed by qRT-PCR. The flavonoid biosynthesis-related gene BnF3H was significantly up-regulated in the roots of hydroponic ramie, and higher total flavonoids content in the hydroponic ramie roots was also observed. The large dataset of transcripts and unigenes provided abundant genetic information for identifying flavonoid biosynthesis genes in ramie roots. Our results suggest flavonoid biosynthesis in ramie roots is positively affected in aquatic environment, and hydroponic culturing could have a potential for enhancing total flavonoids content in ramie roots.

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References

  • André CM, Schafleitner R, Legay S, Lefèvre I, Aliaga CAA, Nomberto G, Hoffmann L, Hausman JF, Larondelle Y, Evers D (2009) Gene expression changes related to the production of phenolic compounds in potato tubers grown under drought stress. Phytochemistry 70(9):1107–1116

    Article  PubMed  Google Scholar 

  • Bakar MFA, Mohamed M, Rahmat A, Fry J (2009) Phytochemicals and antioxidant activity of different parts of bambangan (Mangifera pajang) and tarap (Artocarpus odoratissimus). Food Chem 113(2):479–483

    Article  Google Scholar 

  • Beno-Moualem D, Vinokur Y, Prusky D (2001) Cytokinins increase epicatechin content and fungal decay resistance in avocado fruits. J Plant Growth Regul 20(1):95–100

    Article  CAS  Google Scholar 

  • Boudet AM (2007) Evolution and current status of research in phenolic compounds. Phytochemistry 68(22–24):2722–2735

    Article  CAS  PubMed  Google Scholar 

  • Castellarin SD, Matthews MA, Gaspero GD, Gambetta GA (2007) Water deficits accelerate ripening and induce changes in gene expression regulating flavonoid biosynthesis in grape berries. Planta 227(1):101–112

    Article  CAS  PubMed  Google Scholar 

  • Chappelle EW, Kim MS, Iii MM (1992) Ratio analysis of reflectance spectra (RARS): an algorithm for the remote estimation of the concentrations of chlorophyll A, chlorophyll B, and carotenoids in soybean leaves. Remote Sens Environ 39(3):239–247

    Article  Google Scholar 

  • Chen Y, Wang G, Hong W, Cheng C, Zang G, Guo X, Rui HL (2014) Phytochemical profiles and antioxidant activities in six species of ramie leaves. PLoS ONE 9(9):e108140

    Article  PubMed  PubMed Central  Google Scholar 

  • Conesa A, Götz S (2008) Blast2GO: a comprehensive suite for functional analysis in plant genomics. Int J Plant Genomics 2008(2008):619832

    PubMed  Google Scholar 

  • Gao G, Xiong H, Chen J, Chen K, Chen P, Yu C, Zhu A (2018) Hydroponic method for ramie and removal of nitrogen and phosphorus from livestock wastewater. Int J Phytorem 20(6):545–551

    Article  CAS  Google Scholar 

  • Goda K, Sreekala MS, Gomes A, Kaji T, Ohgi J (2006) Improvement of plant based natural fibers for toughening green composites—effect of load application during mercerization of ramie fibers. Compos A Appl Sci Manuf 37(12):2213–2220

    Article  Google Scholar 

  • Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng Q (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29(7):644

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Huang J, Liang X, Xuan Y, Geng C, Li Y, Lu H, Qu S, Mei X, Chen H, Yu T (2017) A reference human genome dataset of the BGISEQ-500 sequencer. Gigascience 6(5):1–9

    Article  PubMed  PubMed Central  Google Scholar 

  • Jaini R, Wang P, Dudareva N, Chapple C, Morgan J (2017) Targeted metabolomics of the phenylpropanoid pathway in Arabidopsis thaliana using reversed phase liquid chromatography coupled with tandem mass spectrometry. Phytochem Anal 28(4):267–276

    Article  CAS  PubMed  Google Scholar 

  • Kitamura S (2006) Transport of flavonoids: from cytosolic synthesis to vacuolar accumulation. In: Grotewold E (ed) The science of flavonoids. Springer, New York, pp 123–146

  • Koes R, Verweij W, Quattrocchio F (2005) Flavonoids: a colorful model for the regulation and evolution of biochemical pathways. Trends Plant Sci 10(5):236–242

    Article  CAS  PubMed  Google Scholar 

  • Koes RE, Quattrocchio F, Mol JNM (1994) The flavonoid biosynthetic pathway in plants: function and evolution. BioEssays 16(2):123–132

    Article  CAS  Google Scholar 

  • Lee DG, Cho S, Lee J, Yang S, Jung YS, Kim HB, Cho EJ, Lee S (2015) Quantitative analysis of the flavonoid content in the leaves of Boehmeria nivea and related commercial products. Nat Prod Sci 21(1):66–70

    CAS  Google Scholar 

  • Lee H, Joo N (2012) Optimization of pan bread prepared with ramie powder and preservation of optimized pan bread treated by gamma irradiation during storage. Prev Nutr Food Sci 17(1):53–63

    Article  PubMed  PubMed Central  Google Scholar 

  • Lin CC, Yen MH, Lo TS, Lin CF (1997) The antiinflammatory and liver protective effects of Boehmeria nivea and B. nivea subsp. nippononivea in rats. Phytomed Int J Phytother Phytopharmacol 4(4):301

    CAS  Google Scholar 

  • Liu M, Li X, Liu Y, Cao B (2013) Regulation of flavanone 3-hydroxylase gene involved in the flavonoid biosynthesis pathway in response to UV-B radiation and drought stress in the desert plant, Reaumuria soongorica. Plant Physiol Biochem Ppb 73(6):161

    Article  CAS  PubMed  Google Scholar 

  • Morales LO, Tegelberg R, Brosche M, Keinanen M, Lindfors A, Aphalo PJ (2009) Effects of solar UV-A and UV-B radiation on gene expression and phenolic accumulation in Betula pendula leaves. Comp Biochem Physiol A Mol Integr Physiol 153:2–923

    Article  Google Scholar 

  • Madhava Rao KV, Sresty TV (2000) Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Sci 157(1):113–128

    Article  CAS  PubMed  Google Scholar 

  • Mak SST, Gopalakrishnan S, Caroe C, Geng C, Liu S, Sinding MS, Kuderna LFK, Zhang W, Fu S, Vieira FG, Germonpre M, Bocherens H, Fedorov S, Petersen B, Sicheritz-Ponten T, Marques-Bonet T, Zhang G, Jiang H, Gilbert MTP (2017) Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing. Gigascience 6(8):1–13

    Article  PubMed  PubMed Central  Google Scholar 

  • Morozova O, Hirst M, Marra MA (2009) Applications of new sequencing technologies for transcriptome analysis. Annu Rev Genomics Hum Genet 10(10):135

    Article  CAS  PubMed  Google Scholar 

  • Mortazavi A, Williams BA, Mccue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods 5(7):621

    Article  CAS  PubMed  Google Scholar 

  • Nishihara M, Nakatsuka T, Yamamura S (2005) Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene. FEBS Lett 579(27):6074–6078

    Article  CAS  PubMed  Google Scholar 

  • Park JS, Choung MG, Kim JB, Hahn BS, Kim JB, Bae SC, Roh KH, Kim YH, Cheon CI, Sung MK (2007) Genes up-regulated during red coloration in UV-B irradiated lettuce leaves. Plant Cell Rep 26(4):507–516

    Article  CAS  PubMed  Google Scholar 

  • Peng M, Shahzad R, Gul A, Subthain H, Shen S, Lei L, Zheng Z, Zhou J, Lu D, Wang S, Nishawy E, Liu X, Tohge T, Fernie AR, Luo J (2017) Differentially evolved glucosyltransferases determine natural variation of rice flavone accumulation and UV-tolerance. Nat Commun 8(1):1975

    Article  PubMed  PubMed Central  Google Scholar 

  • Quettierdeleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, Cazin M, Cazin JC, Bailleul F, Trotin F (2000) Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol 72(1–2):35

    Article  CAS  Google Scholar 

  • Sparvoli F, Martin C, Scienza A, Gavazzi G, Tonelli C (1994) Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.). Plant Mol Biol 24(5):743

    Article  CAS  PubMed  Google Scholar 

  • Strauss SY, Whittal JB (2006) Non-pollinator agents of selection on floral traits. Ecol Evol Flowers 208:120–139

    Google Scholar 

  • Von Wettberg EJ, Stanton ML, Whittall JB (2010) How anthocyanin mutants respond to stress: the need to distinguish between stress tolerance and maximal vigour. Evol Ecol Res 12(4):457–476

    Google Scholar 

  • WinkelShirley B (2001) Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol 126(2):485–493

    Article  CAS  Google Scholar 

  • Yao L, Wang J, Li B, Meng Y, Ma X, Si E, Ren P, Yang K, Shang X, Wang H et al (2017) Transcriptome sequencing and comparative analysis of differentially-expressed isoforms in the roots of Halogeton glomeratus under salt stress. Gene 649:159–168

    Google Scholar 

  • Zhang Y, Xia H, Yuan M, Zhao C, Li A, Wang X (2012) Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene. Electron J Biotechnol 15(15):5–5

    Google Scholar 

Download references

Acknowledgements

This work was supported by the Agricultural Science and Technology Innovation Project of Chinese under Grant Number CAAS-ASTIP-2018, and China Agricultural Research System under Grant Number CARS-16 and National Natural Science Foundation of China under Grant Number 31701477.

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Authors and Affiliations

  1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China

    Gang Gao, Ping Chen, Jikang Chen, Kunmei Chen, Heping Xiong, Chunming Yu & Aiguo Zhu

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  1. Gang Gao
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  2. Ping Chen
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  3. Jikang Chen
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  4. Kunmei Chen
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  5. Heping Xiong
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Correspondence to Chunming Yu or Aiguo Zhu.

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Gao, G., Chen, P., Chen, J. et al. Expression profile of genes involved in ramie flavonoids biosynthesis pathway and regulation of flavanone 3-hydroxylase (BnF3H) in response to aquatic environment. Acta Physiol Plant 42, 60 (2020). https://doi.org/10.1007/s11738-020-03036-w

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  • DOI: https://doi.org/10.1007/s11738-020-03036-w

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