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Novel antimicrobial and antioxidative activity by endophytic Penicillium roqueforti and Trichoderma reesei isolated from Solanum surattense

Acta Physiologiae Plantarum volume 41, Article number: 164 (2019) Cite this article

Abstract

Crop protection against phyto-pathogens has become a global challenge that can be tackled efficiently through natural resources, including endophytic fungi. Endophytes serve as a reservoir for the vast array of potent bioactive metabolites. We investigated the antioxidant and antibacterial potency of endophytes from the roots of Solanum surattense. The non-polar fraction of the cultural filtrate from the isolated strains was tried for antibacterial potency through agar plate diffusion assay. Among the isolated strains, Penicillium roqueforti (CGF-1) and Trichoderma reesei (CGF-11) had broad-spectrum antibacterial activity against phyto-pathogenic bacteria (Xanthomonas oryzae, Pseudomonas syringae, Agrobacterium tumefaciens, and Ralstonia solanacearum). The extracts of CGF-1 and CGF-11 achieved the best result against A. tumefaciens. Similarly, qualitative analysis of the ethyl acetate extracts P. roqueforti and T. reesei exposed the occurrence of alkaloids, flavonoids, phenols, steroids, and tannins. HPLC analysis also confirmed the presence ferulic acid, cinnamic acid, quercetin, and rutin in the non-polar fraction of the cultural filtrate from the isolated strains. The results conclude that P. roqueforti and T. reesei can play an active role against the plant pathogens by secreting the bioactive compounds to protect host plant. Furthermore, the antibacterial and antioxidant potential of the P. roqueforti and T. reesei suggests its use in agriculture and pharmaceutical industry.

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Data availability statement

The 1TS rDNA sequence was submitted to NCBI GenBank and was assigned Accession No. KY173360 for CGF-1 and KY100257.1 for CGF-11. All other data are included in the manuscript.

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Acknowledgements

The research work was supported by the Department of Botany, Abdul Wali Khan University Mardan. The authors are also thankful to PCSIR and NARC for providing the pathogens for this study.

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Authors and Affiliations

  1. Department of Botany, Hazara University, Mansehra, 21120, Pakistan

    Muhammad Ikram & Niaz Ali

  2. Department of Botany, Abdul Wali Khan University Mardan, Mardan, 23200, Pakistan

    Gul Jan, Muhammad Hamayun & Farzana Gul Jan

  3. Department of Agriculture, Abdul Wali Khan University Mardan, Mardan, 23200, Pakistan

    Amjad Iqbal

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  1. Muhammad Ikram
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Correspondence to Amjad Iqbal.

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Ikram, M., Ali, N., Jan, G. et al. Novel antimicrobial and antioxidative activity by endophytic Penicillium roqueforti and Trichoderma reesei isolated from Solanum surattense. Acta Physiol Plant 41, 164 (2019). https://doi.org/10.1007/s11738-019-2957-z

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  • DOI: https://doi.org/10.1007/s11738-019-2957-z

Keywords

  • Endophytic fungi
  • 18S rDNA
  • Antimicrobial activity
  • MIC/MBC
  • Antioxidant compounds