Manipulation of cellular energy reveals the relationship between ultraweak luminescence and cellular energy during senescence of strawberry (Fragaria × ananassa) fruits
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The goal of this study was to investigate the ultraweak luminescence (UWL) of strawberry fruits in relation to mitochondrial functions and energy production during strawberry senescence. Fully ripe strawberry fruits and mitochondria isolated from those fruits were treated with either adenosine triphosphate (ATP) or the respiratory chain uncoupler 2,4-dinitrophenol (DNP). The activities of H+-ATPase and Ca2+-ATPase, the content of ATP, the free radical O2− as well as the UWL intensity were measured. Our results showed that activities of H+-ATPase and Ca2+-ATPase as well as the ATP content gradually decreased during fruit senescence in all three groups. Compared with the control, DNP treatment exacerbated, while ATP treatment reduced the decrease of H+-ATPase and Ca2+-ATPase activity, the energy charge and UWL intensity. UWL intensity was positively correlated with mitochondrial function and ATP content. Our results strongly suggest that mitochondria are a major source of UWL of strawberry fruits, and that the cellular energy ATP plays important roles in senescence of strawberry fruits, and in UWL formation. Our study provides convincing evidence of the interrelationship between cellular energy and UWL, which helps researchers to better understand the process of senescence in strawberry fruits.
KeywordsCellular energy Mitochondria Strawberry fruit senescence Ultraweak luminescence
This work was financially supported by the Natural Science Foundation of China (Grant No. 31260455).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
Human and animal rights statement
This study did not involve human participants and/or animals.
- Giampieri F, Alvarez-Suarez JM, Mazzoni L, Forbes-Hernandez TY, Gasparrini M, Gonzàlez-Paramàs AM, Santos-Buelga C, Quiles JL, Bompadre S, Mezzetti B, Battino M (2014) An anthocyanin-rich strawberry extract protects against oxidative stress damage and improves mitochondrial functionality in human dermal fibroblasts exposed to an oxidizing agent. Food Funct 5(8):1939–1948CrossRefPubMedGoogle Scholar
- Giampieri F, Alvarez-Suarez JM, Cordero MD, Gasparrini M, Forbes-Hernandez TY, Afrin S, Santos-Buelga C, González-Paramás AM, Astolfi P, Rubini C, Zizzi A, Tulipani S, Quiles JL, Mezzetti B, Battino M (2017) Strawberry consumption improves aging-associated impairments, mitochondrial biogenesis and functionality through the AMP-activated protein kinase signaling cascade. Food Chem 234:464–471CrossRefPubMedGoogle Scholar
- Guo J, Liu H, Liang S, Zhu G, Bai Y, Li L (2017a) Relationship between reactive oxygen species and ultraweak luminescence in strawberry fruit during senescence under various reactive oxygen regulation treatments. J Fruit Sci 34:363–369Google Scholar
- Guo J, Zhu G, Li L, Liu H, Liang S (2017b) Ultraweak photon emission in strawberry fruit during ripening and aging is related to energy level. Open Life Sci 12:393–398Google Scholar
- Gurvisch AG, Grabje S, Salkind S (1923) Die Natur des spezifischen Erregers der Zellteilung. Arch Entw Mech 100:11–40Google Scholar
- Hou X, Liao X, Li Y, Zhang X, Bu W, Jia Y, Li G (2004) Ultraweak biophoton emission and its mechanism during seed germination of Amaranthus hypochondriacus. Seed 23:23–27Google Scholar
- IuA V, Lvova OF (1964) Ultra-weak luminescence and oxidative phosphorylation. Mitochondria Biofiz 9:506–507Google Scholar
- Li H (2004) The physiological experimental principle and technology of plant. Advanced Education Press, BeijingGoogle Scholar
- Lin G, Huang Z, Zhang C, Zheng C (2008) Changes in ultraweak luminescence intensity, respiration rate and physiological metabolism of chrysanthemum during floral differentiation. Acta Hortic Sin 35:1819–1824Google Scholar
- Liu H, Liao X, Wu L, Jiang J (2006) Effect of heat shock on biophoton and activities of antioxidant enzymes in immature wheat grains. J Food Sci Biotech 25:75–78Google Scholar
- Liu T, Qian Z, Yang E,F, Qu H, Jiang Y (2010) Respiratory activity and energy metabolism of harvested litchi fruit and their relationship to quality deterioration. J Fruit Sci 27:946–951Google Scholar
- Liu H, Liang S, Yan Y, Bai Y, Guo J (2017) The relationship between senescence and ultraweak photon emission under controlling of reactive oxygen and energy in strawberry fruit. Acta Bot Boreal-Occident Sin 37:1182–1188Google Scholar
- Popp FA, Guq L (1992) Recent advances in biophoton research and its applications Singapore: World Scientific 1Google Scholar
- Stawinski J, Grabikowski E, Majchrowicz I (1980) Ultraweak photon emission generated by germination. Biophoton Phys 1:72–100Google Scholar
- Tan S, Xing D, Tang Y, Li D (2000) Spectral studies of ultra-weak biophoton emission from plant’s leaves. Acta Photo Sin 29:961–965Google Scholar
- Wang H (2007) Effect of respiratory metabolism associated enzymes in mitochondria on peach fruit ripening and softening. Yangzhou Univ 21:21–22Google Scholar
- Wang C, Jiang L, Wang L, Zhu W (2013) Analysis and application of ultraweak bioluminescence of serum in dairy cow. Jiangsu Agri Sci 41:187–189Google Scholar
- Yuan Z (2008) Research progress on ultraweak bioluminescence. J Anhui Agri Sci 36:3092–3094Google Scholar
- Yue X, Liu Y, Hu X, Dong Y (2008) The study of ultraweak luminescence of Anabaena Flos-Aquae Chin. J Spectr Lab 25:673–676Google Scholar
- Zhang X, Yang H (2004) Ultraweak bioluminescence of chloroplast and mitochondria in plants. Plant Physiol Com 40:111–114Google Scholar
- Zhang X, Yang H, Li F, Zhang W (2004) Changes in ultraweak luminescence, ATP and active oxygen contents during apricot florescence. J Plant Physiol Mol Biol 30:41–44Google Scholar
- Zhang J, Zhang X, Gong Z (2010) Application of ultraweak bioluminescence in vegetable research. Chin Agri Sci Bull 30:2493–2495Google Scholar
- Zhu G (2015) The change of ultraweak bioluminescence at the process of development and maturation in strawberry fruit and focus on the excitation mechanism of reactive oxygen and energy level. Master thesis, Inner Mongolia Agricultural University. http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CMFD&dbname=CMFD201502&filename=1015428178.nh&v=MTc4NDdTN0RoMVQzcVRyV00xRnJDVVJMS2ZZT1p0Rnl6blVyL0tWRjI2RzdlNkZ0RExwNUViUElSOGVYMUx1eFk=