Maize seed cryo-storage modifies chlorophyll, carotenoid, protein, aldehyde and phenolics levels during early stages of germination
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We recorded the crypreservation effects (direct immersion) on various parameters of early germination stages of maize seeds (0, 7 and 14 days). Percentages of germination; fresh mass of different seedling parts; levels of chlorophyll pigments (a, b); carotenoids; malondialdehyde; other aldehydes; phenolics (cell wall-linked, free) and proteins were determined. Various statistically significant effects of seed exposure to liquid nitrogen (LN) were recorded. Maize seeds did not seem to be affected by LN exposure either visually or regarding fresh weight or germination rate. However, delayed growth was observed in seedlings recovered from cryopreserved seeds. This trend indicated an increase in the effect of seed cryopreservation on growing plants. The most significant effects of LN exposure were recorded in the combined fresh weight of stems and leaves at day 7 of germination and in fresh weights of roots, stems and leaves at day 14. At the biochemical level, numerous indicators varied following LN exposure, but the most significant effects were recorded in carotenoids, malondialdehyde and other aldehyde contents. LN exposure modified 50.0% of indicators in cotyledons, 48.1% in stems and leaves, 38.8% in roots and 11.1% in seeds. LN storage modified 11.1% of the variables measured at day 0 of germination, 37.0% at day 7, and 52.7% at day 14. Field performance of cryostored seed-derived plants should be evaluated to measure the durability of the changes observed.
KeywordsBiochemical changes Cryopreservation Germplasm preservation Liquid nitrogen Zea mays L
This research was supported by the Cuban Ministry for Superior Education. We are grateful to Mrs. Bárbara Valle and Mrs. Julia Martínez for their excellent technical assistance and important experimental suggestions.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interests.
Human and animal rights
This research did not involve experiments with human or animal participants.
Informed consent was obtained from all individual participants included in the study. Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.
- Arguedas M, Perez A, Abdelnour A, Hernandez M, Engelmann F, Martínez ME, Yabor L, Lorenzo JC (2016) Short-term liquid nitrogen storage of maize, common bean and soybean seeds modifies their biochemical composition. Agric Sci 4:6–12Google Scholar
- CIMMYT (2016) Maize genetic resources. Available via: CIMMYT. http://cropgenebank.sgrp.cgiar.org/index.php/maize-mainmenu-361. Accessed 20 Oct 2017
- Coelho SVB, Rosa SDVF., Fernandes JS (2017) Cryopreservation of coffee seeds: a simplified method. Seed Sci Tech 45:638–649Google Scholar
- Engelmann F (2000) Importance of cryopreservation for the conservation of plant genetic resources. In: Engelmann F, Takagi H (eds) Cryopreservation of tropical plant germplasm—current research progress and applications. JIRCAS, Tsukuba, pp 8–20Google Scholar
- Engelmann F, Takagi H (2000) Cryopreservation of tropical plant germplasm. In: Engelmann F, Takagi H (eds) Cryopreservation of tropical plant germplasm—current research progress and applications. JIRCAS, Tsukuba, p 496Google Scholar
- Fernández-Marín B, Milla R, Martín-Robles N, Arc E, Kranner I, Becerril JM, García-Plazaola JI (2014) Side-effects of domestication: cultivated legume seeds contain similar tocopherols and fatty acids but less carotenoids than their wild counterparts. Plant Biol 14:1599Google Scholar
- Forni C, Braglia R, Beninati S, Lentini A, Ronci M, Urbani A, Provenzano B, Frattarelli A, Tabolacci C, Damiano C (2010) Polyamine concentration, transglutaminase activity and changes in protein synthesis during cryopreservation of shoot tips of apple variety Annurca. CryoLetters 31:413–425PubMedGoogle Scholar
- Gonzalez-Arnao MT, Martinez-Montero ME, Cruz-Cruz CA, Engelmann F (2014) Advances in cryogenic techniques for the long-term. Preserv Plant Biodivers 4:129–170Google Scholar
- Gurr S, McPherson J, Bowles D (1992) Lignin and associated phenolic acids in cell walls. In: Wilkinson DL (ed) Molecular plant pathology. Oxford Press, Oxford, pp 51–56Google Scholar
- Harding K, Marzalina M, Krishnapillay B, Nashatul Z, Normah M, Benson E (2000) Molecular stability assessments of trees regenerated from cryopreserved Mahogany (Swietenia macrophylla King.) seed germplasm using non-radioactive techniques to examine the chromatin structure and DNA methylation status of the ribosomal RNA genes. J Trop For Sci 12:149–163Google Scholar
- ISTA (2005) International rules for seed testing. International Seed Testing Association, BassersdorfGoogle Scholar
- Kalaiselvi R, Rajasekar M, Gomathi S (2017) Cryopreservation of plant materials-a review. IJCS 5:560–564Google Scholar
- Lakhanpaul S, Babrekar P, Chandel K (1996) Monitoring studies in onion (Allium cepa L.) seeds retrieved from storage at − 20°C and − 180 °C. CryoLetters 17:219–232Google Scholar
- Matsumoto T (2017) Cryopreservation of plant genetic resources: conventional and new methods. Rev Agri Sci 5:13–20Google Scholar
- Mikuła A, Makowski D, Walters C, Rybczyński JJ (2010) Exploration of cryo-methods to preserve tree and herbaceous fern gametophytes. In: Kumar A, Fernández H, Revilla MA (eds) Working with ferns: issues and applications. Springer, New York, pp 173–192Google Scholar
- Pérez-Rodríguez JL, Escriba RCR, González GYL, Olmedo JLG, Martínez-Montero ME (2017) Effect of desiccation on physiological and biochemical indicators associated with the germination and vigor of cryopreserved seeds of Nicotiana tabacum L. cv. Sancti Spíritus 96. In Vitro Cell Dev Biol-Plant 53:440–448CrossRefGoogle Scholar
- Salisbury FB, Ross CW (1992) Plant Physiology (In Spanish). Wadsworth Publishing, BelmontGoogle Scholar
- Singh N, Kaur A, Shevkani K (2014) Maize: grain structure, composition, milling, and starch characteristics. In: Chaudhary D, Kumar S, Langyan S (eds) Maize: nutrition dynamics and novel uses. Springer, New Delhi, pp 65–76Google Scholar
- Stanwood P, Bass L (1981) Seed germplasm preservation using liquid nitrogen. Seed Sci Technol 9:423Google Scholar
- Tadeo FR, Gómez-Cadenas A (2008) fisiología del estrés. In: Azcón-Bieto J, Talón M (eds) Fundamentos de fisiología vegetal. MCGRAW-HILL, Madrid, pp 577–597Google Scholar
- Uragami A, Lucas M, Ralambosoa J, Renard M, Dereuddre J (1993) Cryopreservation of microspore embryos of soilseed rape (Brassica napus) by dehydration in air with or without alginate encapsulation. CryoLetters 14:83–90Google Scholar
- Zevallos B, Cejas I, Engelmann F, Carputo D, Aversano R, Scarano M, Yanes E, Martínez-Montero M, Lorenzo JC (2014) Phenotypic and molecular characterization of plants regenerated from non-cryopreserved and cryopreserved wild Solanum lycopersicum Mill. seeds. CryoLetters 35:216–225PubMedGoogle Scholar