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Screening of rice landraces (Oryza sativa L.) for seedling stage salinity tolerance using morpho-physiological and molecular markers

  • Md. Tahjib-Ul-Arif
  • M. Abu Sayed
  • Mirza Mofazzal Islam
  • M. Nurealam Siddiqui
  • S. N. Begum
  • M. Afzal Hossain
Original Article
  • 288 Downloads

Abstract

Traditional rice landraces of coastal area in Bangladesh are distinct regarding their phenotype, response to salt stress and yield attributes. With characterization of these landraces, suitable candidate genes for salinity tolerance could be identified to introgress into modern rice varieties. Therefore, the aim of this experiment was to uncover prospective rice landraces tolerant to salinity. Relying on morphological, biochemical and molecular parameters 25 rice genotypes were tested for salt tolerance at germination and seedling stage. At germination stage 0 and 12 dSm−1 salinity were imposed on rice genotypes. Ward’s cluster analysis divided rice genotypes into three clusters (susceptible, moderately tolerant and tolerant) based on the physiological indices. The tolerant rice landraces to salinity were Sona Toly, Nakraji and Komol Bhog. At seedling stage screening was performed following IRRI standard protocol at 12 dSm−1 salinity level. Based on all morphological and biochemical parameters Komol Bhog was identified as the highly salinity tolerant landrace while Bolonga, Sona Toly, Dud Sail, Tal Mugur and Nakraji were found as tolerant to salinity. Molecular characterization using two simple sequence repeats (SSR) markers, viz. RM121 and RM337 displayed Bolonga, Til Kapor, Panbra, Sona Toly, Bina Sail, Komol Bhog, Nakraji, Tilkapur, Gajor Goria and Gota were tolerant landraces through genetic similarity in dendrogram. These identified salt-resistant landraces can be used as promising germplasm resources for breeding salt-tolerant high-yielding rice varieties in future.

Keywords

Landraces Physiological indices Rice Salt stress Simple sequence repeat 

Notes

Acknowledgements

Authors are grateful to Tahmina Akter and Manos Kranti Saha for laboratory help.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  • Md. Tahjib-Ul-Arif
    • 1
  • M. Abu Sayed
    • 2
    • 3
  • Mirza Mofazzal Islam
    • 4
  • M. Nurealam Siddiqui
    • 5
  • S. N. Begum
    • 4
  • M. Afzal Hossain
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyBangladesh Agricultural UniversityMymensinghBangladesh
  2. 2.Department of Biochemistry and Molecular BiologyHajee Mohammad Danesh Science and Technology UniversityDinajpurBangladesh
  3. 3.United Graduate School of Agricultural ScienceIwate UniversityMoriokaJapan
  4. 4.Plant Breeding DivisionBangladesh Institute of Nuclear AgricultureMymensinghBangladesh
  5. 5.Department of Biochemistry and Molecular BiologyBangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipurBangladesh

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