Comparative studies of leaf surface chemical biosynthesis in different tobacco cultivars

  • Mingyue Huang
  • Hongying Zhang
  • Zhaojun Wang
  • Dexin Niu
  • Yanhua Li
  • Hong Cui
Original Article
  • 155 Downloads

Abstract

To understand the differences in the biosynthesis of leaf surface chemicals and their influence on aphid preference for different tobacco cultivars (Nicotiana tabacum), we analyzed the secretory characteristics of glandular trichomes of four commercial cultivars, K326 (flue-cured), Beinhart 1000-1 (cigar), Basma YNOTBS1 (oriental), and Dabai 1 (burley), and their parental species, Nicotiana sylvestris and Nicotiana tomentosiformis. Trichome-type observation showed that K326 and N. sylvestris have three kinds of glandular trichomes (non-glandular, long stalked glandular, and short stalked glandular trichomes), whereas Beinhart 1000-1, Basma YNOTBS1, Dabai 1, and N. tomentosiformis had two kinds of glandular trichomes (long and short stalked glandular trichomes). The gas chromatography–mass spectrometry profiles of leaf exudates indicated that N. tomentosiformis synthesized only labdanoids; N. sylvestris, K326 and Dabai 1 synthesized only cembranoids; and Beinhart 1000-1 and Basma YNOTBS1 synthesized cembranoids and labdanoids. Gene expression pattern analysis revealed that the labdanoid synthesis-related genes NtABS and NtCPS2 were expressed in N. tomentosiformis, Beinhart 1000-1, and Basma YNOTBS1, whereas the cembranoid synthesis-related genes NtCYC and NtCYP71D16 were expressed in N. sylvestris and all four commercial cultivars. Evolutionary analysis indicated that NtCYC and NtCYP71D16 might be phylogenetically originated from N. sylvestris, whereas NtABS and NtCPS2 expressed in Basma YNOTBS1 and Beinhart 1000-1 might be derived from N. tomentosiformis. In addition, aphid attraction (number of aphids) was significantly and positively correlated with the total glandular secretion (r2 = 0.9425, P ≤ 0.05), and it was significantly and positively correlated with amount of CBT-diol (r2 = 0.9224; P ≤ 0.05). These results provide new insights into the biosynthesis of diterpenoids and biotic stress resistance in tobacco.

Keywords

Nicotiana tabacum Nicotiana sylvestris Nicotiana tomentosiformis Glandular trichome Diterpenoid Aphid resistance 

Notes

Acknowledgements

This study was supported by the State Tobacco Monopoly Administration of China [Grant No. 110201401003 (JY-03)], and the Technology Center, China Tobacco Henan Industrial Co., Ltd. [Grant No. ZW2014004].

Supplementary material

11738_2018_2642_MOESM1_ESM.docx (1001 kb)
Supplementary material 1 (DOCX 1001 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  • Mingyue Huang
    • 1
  • Hongying Zhang
    • 1
  • Zhaojun Wang
    • 1
  • Dexin Niu
    • 1
  • Yanhua Li
    • 1
  • Hong Cui
    • 1
  1. 1.Key Laboratory for Tobacco Cultivation of Tobacco Industry, College of Tobacco ScienceHenan Agricultural UniversityZhengzhouChina

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