Effect of auxin producing and phosphate solubilizing bacteria on mobility of soil phosphorus, growth rate, and P acquisition by wheat plants

Abstract

Microorganisms capable of mobilizing phosphate promote plant growth, this activity being frequently accompanied by production of plant hormones auxins. However, the extent of contribution of these characteristics to promotion of plant growth remains unclear. Paenibacillus illinoisensis IB 1087 and Pseudomonas extremaustralis IB-Ki-13-1A strains were selected for their capacity to mobilize phosphates and to synthesize auxins in vitro. The effects of inoculating these bacteria on the content of mobile phosphorus in the soil as well as on the phosphorus and hormone content in wheat plants were studied and the observed responses were related to the changes in plant growth. Inoculation of bacteria into the soil increased P concentration in the plants suggesting their increased capacity for the efficient acquisition of phosphorus compounds, while concentration of mobile phosphorus in the soil was increased by its inoculation with bacteria only in the absence of plants. The treatment increased plants mass (to greater extent in the case of P. illinoisensis) in accordance with the increased level of auxins in the treated plant. Increased mass accumulation did not correlate with the potential ability of bacteria strains for production of auxins or phosphate mobilization in vitro. Our data indicate importance of increased auxin content in the plants for the stimulation of root growth and capacity for P uptake as influenced by growth-promoting bacteria.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3

References

  1. Allen S, Grimshay HM, Parkinson JA, Quarmby C (1974) Chemical analysis of ecological materials. Blackwell, London

    Google Scholar 

  2. Arkhipova TN, Veselov SU, Melentiev AI, Martynenko EV, Kudoyarova GR (2005) Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants. Plant Soil 272:201–209

    CAS  Article  Google Scholar 

  3. Belimov AA, Kojemiakov AP, Chuvarliyeva CV (1995) Interaction between barley and mixed cultures of nitrogen fixing and phosphate-solubilizing bacteria. Plant Soil 173:29–37

    CAS  Article  Google Scholar 

  4. Contesto C, Milesi S, Mantelin S, Zancarini A, Desbrosses G, Varoquaux F, Bellini C, Kowalczyk M, Touraine B (2010) The auxin-signaling pathway is required for the lateral root response of Arabidopsis to the rhizobacterium Phyllobacterium brassicacearum. Planta 232:1455–1470

    CAS  Article  PubMed  Google Scholar 

  5. Francis I, Holsters M, Vereecke D (2010) The Gram-positive side of plant–microbe interactions. Environ Microbiol 12(1):1–12

    CAS  Article  PubMed  Google Scholar 

  6. King EO, Ward MK, Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307

    CAS  PubMed  Google Scholar 

  7. Kisiel A, Kępczyńska E (2016) Medicago truncatula Gaertn. as a model for understanding the mechanism of growth promotion by bacteria from rhizosphere and nodules of alfalfa. Planta 243:1169–1189

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  8. Kudoyarova GR, Melentiev AI, Martynenko EV, Arkhipova TN, Shendel GV, Kuzmina LU, Dodd IC, Veselov SU (2014) Cytokinin producing bacteria stimulate amino acid deposition by wheat roots. Plant Physiol Biochem 83:285–291

    CAS  Article  PubMed  Google Scholar 

  9. Miyawaki K, Matsumoto-Kitano M, Kakimoto T (2004) Expression of cytokinin biosynthetic isopentenyltransferase genes in Arabidopsis: tissue specificity and regulation by auxin, cytokinin, and nitrate. Plant J 37:128–138

    CAS  Article  PubMed  Google Scholar 

  10. Ortíz-Castro R, Contreras-Cornejo HA, Macías-Rodríguez L, López-Bucio J (2009) The role of microbial signals in plant growth and development. Plant Signal Behav 4(8):701–712

    Article  PubMed  PubMed Central  Google Scholar 

  11. Pikovsky RI (1948) Mobilization of phosphorous in connection with the vital activity of some microbial species. Microbiologia 17:362–370

    Google Scholar 

  12. Richardson AE, Barea JM, Mc Neill AM, Prigent-Combaret C (2009) Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil 321:305–339

    CAS  Article  Google Scholar 

  13. Rodríguez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339

    Article  PubMed  Google Scholar 

  14. Spaepen S, Vanderleyden J, Remans R (2007) Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev 31:425–448

    CAS  Article  PubMed  Google Scholar 

  15. Tanaka M, Takei K, Kojima M, Sakakibara H, Mori H (2006) Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J 45:1028–1036

    CAS  Article  PubMed  Google Scholar 

  16. Veselov SU, Kudoyarova GR, Egutkin NL, Gyuli-Zade V, Mustafma A, Kof E (1992) Modified solvent partitioning scheme providing increased specificity and rapidity of immunoassay for indole 3-acetic acid. Physiol Plant 86:93–96

    CAS  Article  Google Scholar 

  17. Vysotskaya LB, Timergalina LN, Symonyan MV, Veselov SU, Kudoyarova GR (2001) Growth rate, IAA and cytokinin content of wheat seedlings after root pruning. Plant Growth Regul 33:51–57

    CAS  Article  Google Scholar 

  18. Vysotskaya LB, Korobova AV, Kudoyarova GR (2008) Abscisic acid accumulation in the roots of nutrient-limited plants: its impact on the differential growth of roots and shoots. J Plant Physiol 165:1274–1279

    CAS  Article  PubMed  Google Scholar 

  19. Wakelin SA, Anstis ST, Warren RA, Ryder MH (2006) The role of pathogen suppression on the growth promotion of wheat by Penicillium radicum. Australas Plant Path 35(2):253–258

    Article  Google Scholar 

  20. Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmulling T (2003) Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity. Plant Cell 15:2532–2550

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  21. Weyens N, Van der Lelie D, Taghavi S, Newman L, Vangronsveld J (2009) Exploiting plant–microbe partnerships to improve biomass production and remediation. Trends Biotechnol 27(10):591–598

    CAS  Article  PubMed  Google Scholar 

  22. Whitelaw MA, Harden TJ, Helyar KR (1999) Phosphate solubilization in solution culture by the soil fungus Penicillium radicum. Soil Biol Biochem 31(5):655–665

    CAS  Article  Google Scholar 

Download references

Acknowledgements

The work was partially supported by Russian Foundation for Basic Research (Grants 14-04-97049; 15-04-04750).

Author information

Affiliations

Authors

Corresponding author

Correspondence to Guzel R. Kudoyarova.

Additional information

Communicated by MJ Reigosa.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kudoyarova, G.R., Vysotskaya, L.B., Arkhipova, T.N. et al. Effect of auxin producing and phosphate solubilizing bacteria on mobility of soil phosphorus, growth rate, and P acquisition by wheat plants. Acta Physiol Plant 39, 253 (2017). https://doi.org/10.1007/s11738-017-2556-9

Download citation

Keywords

  • Plant growth-promoting rhizobacteria (PGPR)
  • Phosphorus
  • Auxin
  • Triticum durum
  • Growth