Abstract
Microorganisms capable of mobilizing phosphate promote plant growth, this activity being frequently accompanied by production of plant hormones auxins. However, the extent of contribution of these characteristics to promotion of plant growth remains unclear. Paenibacillus illinoisensis IB 1087 and Pseudomonas extremaustralis IB-Ki-13-1A strains were selected for their capacity to mobilize phosphates and to synthesize auxins in vitro. The effects of inoculating these bacteria on the content of mobile phosphorus in the soil as well as on the phosphorus and hormone content in wheat plants were studied and the observed responses were related to the changes in plant growth. Inoculation of bacteria into the soil increased P concentration in the plants suggesting their increased capacity for the efficient acquisition of phosphorus compounds, while concentration of mobile phosphorus in the soil was increased by its inoculation with bacteria only in the absence of plants. The treatment increased plants mass (to greater extent in the case of P. illinoisensis) in accordance with the increased level of auxins in the treated plant. Increased mass accumulation did not correlate with the potential ability of bacteria strains for production of auxins or phosphate mobilization in vitro. Our data indicate importance of increased auxin content in the plants for the stimulation of root growth and capacity for P uptake as influenced by growth-promoting bacteria.
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References
Allen S, Grimshay HM, Parkinson JA, Quarmby C (1974) Chemical analysis of ecological materials. Blackwell, London
Arkhipova TN, Veselov SU, Melentiev AI, Martynenko EV, Kudoyarova GR (2005) Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants. Plant Soil 272:201–209
Belimov AA, Kojemiakov AP, Chuvarliyeva CV (1995) Interaction between barley and mixed cultures of nitrogen fixing and phosphate-solubilizing bacteria. Plant Soil 173:29–37
Contesto C, Milesi S, Mantelin S, Zancarini A, Desbrosses G, Varoquaux F, Bellini C, Kowalczyk M, Touraine B (2010) The auxin-signaling pathway is required for the lateral root response of Arabidopsis to the rhizobacterium Phyllobacterium brassicacearum. Planta 232:1455–1470
Francis I, Holsters M, Vereecke D (2010) The Gram-positive side of plant–microbe interactions. Environ Microbiol 12(1):1–12
King EO, Ward MK, Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307
Kisiel A, Kępczyńska E (2016) Medicago truncatula Gaertn. as a model for understanding the mechanism of growth promotion by bacteria from rhizosphere and nodules of alfalfa. Planta 243:1169–1189
Kudoyarova GR, Melentiev AI, Martynenko EV, Arkhipova TN, Shendel GV, Kuzmina LU, Dodd IC, Veselov SU (2014) Cytokinin producing bacteria stimulate amino acid deposition by wheat roots. Plant Physiol Biochem 83:285–291
Miyawaki K, Matsumoto-Kitano M, Kakimoto T (2004) Expression of cytokinin biosynthetic isopentenyltransferase genes in Arabidopsis: tissue specificity and regulation by auxin, cytokinin, and nitrate. Plant J 37:128–138
Ortíz-Castro R, Contreras-Cornejo HA, Macías-Rodríguez L, López-Bucio J (2009) The role of microbial signals in plant growth and development. Plant Signal Behav 4(8):701–712
Pikovsky RI (1948) Mobilization of phosphorous in connection with the vital activity of some microbial species. Microbiologia 17:362–370
Richardson AE, Barea JM, Mc Neill AM, Prigent-Combaret C (2009) Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil 321:305–339
Rodríguez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339
Spaepen S, Vanderleyden J, Remans R (2007) Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev 31:425–448
Tanaka M, Takei K, Kojima M, Sakakibara H, Mori H (2006) Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J 45:1028–1036
Veselov SU, Kudoyarova GR, Egutkin NL, Gyuli-Zade V, Mustafma A, Kof E (1992) Modified solvent partitioning scheme providing increased specificity and rapidity of immunoassay for indole 3-acetic acid. Physiol Plant 86:93–96
Vysotskaya LB, Timergalina LN, Symonyan MV, Veselov SU, Kudoyarova GR (2001) Growth rate, IAA and cytokinin content of wheat seedlings after root pruning. Plant Growth Regul 33:51–57
Vysotskaya LB, Korobova AV, Kudoyarova GR (2008) Abscisic acid accumulation in the roots of nutrient-limited plants: its impact on the differential growth of roots and shoots. J Plant Physiol 165:1274–1279
Wakelin SA, Anstis ST, Warren RA, Ryder MH (2006) The role of pathogen suppression on the growth promotion of wheat by Penicillium radicum. Australas Plant Path 35(2):253–258
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmulling T (2003) Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity. Plant Cell 15:2532–2550
Weyens N, Van der Lelie D, Taghavi S, Newman L, Vangronsveld J (2009) Exploiting plant–microbe partnerships to improve biomass production and remediation. Trends Biotechnol 27(10):591–598
Whitelaw MA, Harden TJ, Helyar KR (1999) Phosphate solubilization in solution culture by the soil fungus Penicillium radicum. Soil Biol Biochem 31(5):655–665
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The work was partially supported by Russian Foundation for Basic Research (Grants 14-04-97049; 15-04-04750).
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Communicated by MJ Reigosa.
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Kudoyarova, G.R., Vysotskaya, L.B., Arkhipova, T.N. et al. Effect of auxin producing and phosphate solubilizing bacteria on mobility of soil phosphorus, growth rate, and P acquisition by wheat plants. Acta Physiol Plant 39, 253 (2017). https://doi.org/10.1007/s11738-017-2556-9
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DOI: https://doi.org/10.1007/s11738-017-2556-9