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Ca2+-ATPase in the symbiosome membrane from broad bean root nodules: further evidence for its functioning as ATP-driven Ca2+/H+ exchanger

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Abstract

To date, it has been established that the symbiosome membrane (SM), i.e., plant-derived membrane of symbiosomes, nitrogen-fixing compartments of legume root nodules, is equipped with Ca2+-ATPase transporting Ca2+ ions through the SM from the cytosol of infected cells into the symbiosome space (SS). Earlier in the experiments on the SM vesicles isolated from broad bean root nodules some data indicating the action of the Ca2+-ATPase as ATP-driven Ca2+/H+ antiporter were obtained. In the present work performed on isolated symbiosomes from the same plant object, further evidence in favor of calcium-proton countertransport mechanism of the pump operation was obtained. These were expressed in vanadate-sensitive alkalinization of the SS coupled with Ca2+ uptake by symbiosomes catalyzed by the SM Ca2+-ATPase, stimulation of the kinetics of the latter process in the response to artificial acidification of the SS and expectable modulation of ITP-hydrolyzing activity of this enzyme caused by the variation of pH within this compartment. The above findings are discussed in the framework of the model describing the mechanism of Ca2+-ATPase operation as an ATP-driven Ca2+/H+ exchanger and on this base allow us to put forward the hypothesis about the involvement of this enzyme in symbiosome signaling in a Ca2+- and pH-dependent manner.

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Abbreviations

AO:

Amine dye acridine orange

HEPES:

N-2-hydroxyethylpiperazine-N′-ethanesulphonic acid

BTP:

1,3-Bis (tris (hydroxymethyl)-methylamino) propane

SM:

Symbiosome membrane

SS:

Symbiosome space

Na2EDTA:

Ethylene diamine tetraacetic acid disodium salt

EGTA:

Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid

FCCP:

Carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone

MES:

2-(N-morpholino) ethanesulfonic acid

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Acknowledgements

This work was supported by a Grant No. 15-04-02451 from Russian Foundation for Basic Research.

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya st. 35, Moscow, 127276, Russia

    Valeriya V. Krylova, Igor M. Andreev, Rosaliya Zartdinova & Stanislav F. Izmailov

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  1. Valeriya V. Krylova
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  2. Igor M. Andreev
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  3. Rosaliya Zartdinova
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  4. Stanislav F. Izmailov
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Correspondence to Valeriya V. Krylova.

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Communicated by L. A. Kleczkowski.

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Krylova, V.V., Andreev, I.M., Zartdinova, R. et al. Ca2+-ATPase in the symbiosome membrane from broad bean root nodules: further evidence for its functioning as ATP-driven Ca2+/H+ exchanger. Acta Physiol Plant 39, 247 (2017). https://doi.org/10.1007/s11738-017-2546-y

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