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Aluminum toxicity-induced alterations in the leaf proteome of rice contrasting response towards inoculation of plant growth-promoting bacteria

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Abstract

Aluminum (Al) toxicity is one of the primary factors limiting crop production on acid sulfate soils. In the present study, two-dimensional polyacrylamide gel electrophoresis approach (2D-PAGE) was applied to identify Al tolerance, plant growth promotion and separately expressed proteins were determined using mass spectrometry. Seedlings of rice were grown in an acid sulfate soil. Bio-fertilizer containing acid-tolerant and plant growth-promoting bacteria (PGPB) were given alone or in combination with ground magnesium limestone (GML) at 4 t ha−1. Leaf samples were taken at 45 days after transplanting. Results exhibited that the application of bio-fertilizer and GML increased soil pH and leaf chlorophyll content. Highest plant height, root length and tiller number were observed in the modified treatments. A total of eight different proteins have been identified to be differentially expressed in rice leaf tissues by mass spectrometry analysis that exposed the differential expression of certain vital proteins. The identified proteins were involved in tolerating abiotic stress, disease resistance, oxidation–reduction process and internal plant physiological functions. Results of analyzing multiple protein spots have shown their involvement for the promotion of plant development and reduction of Al toxicity. The study thus confirms the primary role of PGPB containing bio-fertilizer with GML on growth of rice under Al toxic conditions in acid sulfate soil.

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Abbreviations

2D DIGE:

Two-dimensional differential in gel electrophoresis

CHAPS:

3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate

CI%:

Confidence interval percentage

DTT:

Dithiothreitol

GML:

Ground magnesium limestone

IPG:

Immobilized pH gradient

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

TCA cycle:

Tricarboxylic acid cycle

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Acknowledgements

The authors acknowledge the technical support provided by Universiti Putra Malaysia and the Long Term Research Grant Scheme (LRGS) fund for Food Security by the Ministry of Higher Education Malaysia for providing the financial support.

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Authors and Affiliations

  1. Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Qurban Ali Panhwar & Mohd Razi Ismail

  2. Soil and Environmental Sciences Division, Nuclear Institute of Agriculture, Tandojam, Sindh, 70060, Pakistan

    Qurban Ali Panhwar

  3. Soil Science Division, Bangladesh Rice Research Institute, Gazipur, 1701, Bangladesh

    Umme Aminun Naher

  4. Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Radziah Othman & Jusop Shamshuddin

  5. Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

    Radziah Othman & Khalid Rehman Hakeem

  6. Laboratory of Food Crop, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    Norazrin Ariffin

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  1. Qurban Ali Panhwar
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  2. Umme Aminun Naher
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Correspondence to Radziah Othman.

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Communicated by Z.-L. Zhang.

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Panhwar, Q.A., Naher, U.A., Othman, R. et al. Aluminum toxicity-induced alterations in the leaf proteome of rice contrasting response towards inoculation of plant growth-promoting bacteria. Acta Physiol Plant 39, 214 (2017). https://doi.org/10.1007/s11738-017-2498-2

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  • DOI: https://doi.org/10.1007/s11738-017-2498-2

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