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Recombinant pharmaceutical protein production in plants: unraveling the therapeutic potential of molecular pharming

Acta Physiologiae Plantarum volume 39, Article number: 18 (2017) Cite this article

Abstract

There is an increasing demand for the generation of recombinant pharmaceutical proteins for a wide array of therapeutic applications. In comparison to bacterial, yeast and animal cells, the production of recombinant proteins in plants with economic and therapeutic importance has only started recently. The most important prerequisite of any expression systems is that it should be simple and inexpensive. In this regard, plant-based expression has emerged an as accepted alternative to conventional expression platforms due to economic feasibility, rapid scalability, higher stability of recombinant proteins, safety due to lack of harmful substances (human, animal pathogens and pyrogens) and capability of producing proteins with desired secondary modifications. Heterologous expression using plants has played a pivotal role in the development of a myriad of recombinant proteins, including neutraceuticals and monoclonal antibodies being utilized in various therapeutic approaches. This paper presents an overview about the current status, various strategies and advantages of pharmaceutical protein production in plant expression systems. We also present a summary of expression of therapeutic monoclonal antibodies, vaccines, clinical trials and the regulatory aspects of plant-based expression. Furthermore, the challenges encountered in plant expression such as costs associated with existing purification strategies are discussed.

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Abbreviations

AAD:

Antibiotic-associated diarrhea

Mab:

Monoclonal antibody

ScFv:

Single chain variable fragment

PMP:

Plant-made pharmaceuticals

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Acknowledgements

The authors thank Vignan’s University, India for its in-house administrative and financial resources to execute this work. This work was supported in part by a Grant (ECR/2016/000304) from Science and Engineering Research Board, New Delhi, India under the young scientist scheme. We also thank the anonymous reviewers for their comments which helped us in improving the quality of manuscript. We thank Dr. David McMurray, Regents Professor, Texas A & M University System Health Science Center, College Station, USA for providing valuable comments.

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Authors and Affiliations

  1. Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research University (VFSTRU), Guntur, 522213, Andhra Pradesh, India

    Vijaya R. Dirisala, Krupanidhi Srirama, Prakash Narayana Reddy & N. Satya Sampath Kumar

  2. Department of Biotechnology, Acharya Nagarjuna University, Guntur, 522510, Andhra Pradesh, India

    K. R. S. Sambasiva Rao

  3. Plant Cell Biotechnology Department, Central Food Technological Research Institute (CFTRI), Mysore, 570013, Karnataka, India

    Giridhar Parvatam

  4. Administrative office, Aushmath Biosciences, Devaraj Corner, Vadavalli Post, Coimbatore, 641046, Tamil Nadu, India

    Rahul R. Nair

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  1. Vijaya R. Dirisala
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  3. Krupanidhi Srirama
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Correspondence to Vijaya R. Dirisala.

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Communicated by A. K. Kononowicz.

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Dirisala, V.R., Nair, R.R., Srirama, K. et al. Recombinant pharmaceutical protein production in plants: unraveling the therapeutic potential of molecular pharming. Acta Physiol Plant 39, 18 (2017). https://doi.org/10.1007/s11738-016-2315-3

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