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Comparative shotgun proteomic profiles for identification of differentially expressed proteins in response to topping in Nicotiana tabacum leaves

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Abstract

The aim of this study was to explore the differences of protein expression in tobacco leaves in response to topping. Comparative shotgun proteomic approach combined with mass spectrometry was used to screen the differentially expressed proteins between leaves before topping and leaves after topping. Additionally, quantitative real-time PCR was applied to validate the differentially expressed proteins. A total of 198 and 193 differentially expressed proteins were identified in leaves before and after topping, respectively. Among them, 64 and 59 proteins were uniquely identified in leaves before and after topping, respectively. In leaves before topping, uniquely expressed proteins were mainly involved in photosynthesis and ATPase energy. In leaves after topping, uniquely expressed proteins (such as glucan endo-1,3-beta-glucosidase acidic isoform, GI9 and pathogenesis-related protein IB) were mainly involved in resistance/defense. Moreover, uniquely expressed proteins (such as cytosolic acetoacetyl-coenzyme A thiolase and plastid transketolase) were related to secondary metabolism. The outcomes demonstrated that topping may weaken the expressions of proteins related to energy and synthesis metabolism, as well as trigger the expressions of proteins involved in defense and secondary metabolism.

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Acknowledgements

This work was supported by grants of Major Project from State Tobacco Monopoly Administration of China (No. TS-01-201102) and the Natural Science Fundamental Research Program of the Henan Education Department (No. 2013A180474).

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Authors and Affiliations

  1. College of Tobacco Science, Henan Agricultural University, No. 63 Nongye Road, Zhengzhou, 450002, People’s Republic of China

    Huijuan Yang, Fei Su, Jing Wang & Hongzhi Shi

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  1. Huijuan Yang
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  2. Fei Su
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  3. Jing Wang
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  4. Hongzhi Shi
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Correspondence to Hongzhi Shi.

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Additional information

Communicated by S. Abe.

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11738_2016_2308_MOESM1_ESM.tif

Supplementary material 1 (TIFF 286 kb) Supplementary Fig. 1 The total ion chromatograms of all protein samples. A: pro-topping; B: post-topping

11738_2016_2308_MOESM2_ESM.tif

Supplementary material 2 (TIFF 433 kb) Supplementary Fig. 2 The typical mass spectrograms of leaves before (A) and after (B) decapitation. The precursor ion in (A) was 870.82, 511.45, 644.60 and 942.99, while that in (B) was 760.12, 379.57, 602.37 and 726.18, respectively

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Supplementary material 4 (XLS 628 kb)

Supplementary material 5 (XLS 594 kb)

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Yang, H., Su, F., Wang, J. et al. Comparative shotgun proteomic profiles for identification of differentially expressed proteins in response to topping in Nicotiana tabacum leaves. Acta Physiol Plant 39, 13 (2017). https://doi.org/10.1007/s11738-016-2308-2

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  • DOI: https://doi.org/10.1007/s11738-016-2308-2

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