Abstract
Chrysanthemum morifolium ‘Hangju’ is a valuable medicinal plant. We previously reported cryopreservation of shoot tips of C. morifolium ‘Hangju’. The present study further evaluated greenhouse performance, assessed genetic stability and analyzed biochemical compounds in the greenhouse-grown plants regenerated from cryopreservation. The results showed that although some minor alternations were detected in early vegetative growth, there were no differences in major parameters of vegetative growth and flower production between the plants regenerated from cryopreservation and in vitro shoots (the control). Morphologies of leaves and flowers were identical between the two types of the plants. No polymorphic bands revealed by ISSR and RAPD, and no alternations at ploidy levels analyzed by FCM were found in the cryo-derived plants. The types and number of biochemical compounds analyzed by UPLC-MS/MS were identical between the two types of the plants. Quantitative analyses by HPLC showed no differences in the contents of the five selected biochemical compounds produced between the plants regenerated from cryopreservation and in vitro shoots. Therefore, cryopreservation would provide a technical platform for establishment of cryo-banking of Chrysanthemum germplasm with medicinal values.
Similar content being viewed by others
References
Agrawal A, Swennen R, Panis B (2004) A comparison of four methods for cryopreservation of meristems in banana (Musa spp.). CryoLetters 25:101–110
Ahuja S, Mandal BB, Dixit S, Srivastava PS (2002) Molecular, phenotypic and biosynthetic stability in Dioscorea floribunda plants derived from cryopreserved shoot tips. Plant Sci 5:971–977
Ashmore SE, Drew RA, Kaity A, Hummer KE (2011) Storage stability using cryopreservation: a case study in papaya. Acta Hortic 918:125–130
Benson EE (2008) Cryopreservation of phytodiversity: a critical appraisal of theory and practice. Crit Rev Plant Sci 27:141–219
Chen JY (2012) The origin of garden Chrysanthemum. Anhui Sci Technol Publishing House, Hefei
Chen YY, Zhang SS, Chen DH, Zhou M, Zheng J, Zheng X (2015) An UPLC-MS/MS method for determination of solasonine in rat plasma and its application of a pharmacokinetic and bioavailability study. J Chromatogr B 985:1–5
Chinese Pharmacopoeia Commission (2010) Pharmacopoeia of the People’s Republic of China Version. Chinese Medicine Science Technology Press, Beijing, p 292
Fukai S (1990) Cryopreservation of Chrysanthemum shoot tips. Sci Hortic 45:167–174
Fukai S, Goi M, Tanaka M (1991) Cryopreservation of shoot tips of Chrysanthemum morifolium and related species native to Japan. Euphytica 54:201–204
Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell-cycle in intact plant-tissues. Science 220:1049–1051
Halmagyi A, Fischer-klüver G, Mix-Wagner G, Schumacher HM (2004) Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches. Plant Cell Rep 22:371–375
Harding K (2004) Genetic integrity of cryopreserved plant cells: a review. CryoLetters 25:3–22
Harding K, Staines H (2001) Biometric analysis of phenotypic characters of potato shoot-tips recovered from tissue culture, dimethyl sulphoxide treatment and cryopreservation. CryoLetters 22:255–262
Hitmi A, Sallanon H, Barthomeuf C (1997) Cryopreservation of Chrysanthemum cinerariaefolium Vis. cells and its impact on their pyrethrin biosynthesis ability. Plant Cell Rep 17:60–64
Hitmi A, Barthomeuf C, Sallanon H (1999) Cryopreservation of Chrysanthemum cinerariaefolium shoot tips: effects of pretreatment conditions and retention of biosynthetic capacity. CryoLetters 20:109–120
Hitmi A, Barthomeuf C, Sallanon H (2000) Cryopreservation of Chrysanthemum cinerariaefolium shoot tips. J Plant Physiol 156:408–412
Jiang HD, Wang LF, Zhou XM, Xia Q (2005) Vasorelaxant effects and underlying mechanism of EtOAc extract from Chrysanthemum morifolium in rat thoracicaorta. Chin J Pathophysiol 21:334–338
Kaity A, Ashmore SE, Drew RA (2009) Field performance evaluation and genetic integrity assessment of cryopreserved papaya clones. Plant Cell Rep 28:1421–1430
Keller ERJ, Senula A, Kaczmarczyk A (2008) Cryopreservation of herbaceous dicots. In: Reed BM (ed) Plant cryopreservation: a practical guide. Springer-Verlag GmbH, New York, pp 281–332
Kim HJ, Lee YS (2005) Identification of new antioxidant activities. Planta Med 71:871–876
Lee JS, Kim HJ, Lee YS (2003) A new anti-HIV flavonoid glucuronide from Chrysanthemum morfolium. Planta Med 69:859–861
Lee YG, Popova E, Cui HY, Kim HH, Park SU, Bae CH, Lee SC, Engelmann F (2011) Improved cryopreservation of Chrysanthemum (Chrysanthemum morifolium) using droplet-vitrification. CryoLetters 32:487–497
Martín C, González-Benito ME (2005) Survival and genetic stability of Dendranthema grandiflora Tzvelev shoot apices after cryopreservation by vitrification and encapsulation–dehydration. Cryobiol 51:281–289
Martín C, Cervera MT, González-Benito ME (2011) Genetic stability analysis of Chrysanthemum (Chrysanthemum × morifolium Ramat.) after different stages of an encapsulation–dehydration cryopreservation protocol. J Plant Physiol 168:158–166
Martinez-Montero ME, Ojeda E, Espinosa A, Sanchez M, Castillo R, Gonzalez-Arnao MT, Engelmann F, Lorenzo JC (2002) Field performance of sugarcane (Saccharum sp.) plants derived from cryopreserved calluses. CryoLetters 23:21–26
Medina JJ, Clavero-Ramírez I, González-Benito ME, Gálvez-Farfán J, López-Aranda JM, Soria C (2007) Field performance characterization of strawberry (Fragari ananassa Duch.) plants derived from cryopreserved apices. Sci Hortic 113:28–32
Miñano HS, González-Benito ME, Martín C (2009) Molecular characterization and analysis of somaclonal variation in chrysanthemum cultivars using RAPD markers. Sci Hortic 122:238–243
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assayswith tobacco tissue cultures. Physiol Plant 15:473–497
Peng YR, Shi L, Luo YH, Ding YF (2006) Protective affect of total flavones from chrysanthemum on isoprealine-induced myocaedial ischrmia in rats. Lishizhen Med Mat Med Res 17:1131–1132
Reed BM (2008) Plant cryopreservation: a practical guide. Springer-Verlag GmbH, New York
Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30–33
Sakai A, Matsumoto T, Hirai D, Niino T (2000) Newly developed encapsulation-dehydration protocol for plant cryopreservation. CryoLetters 21:53–62
Teixeira da Silva JA (2003) Chrysanthemum: advances in tissue culture, cryopreservation, postharvest technology, genetics and transgenic biotechnology. Biotechnol Adv 21:715–766
Wang B, Wang R-R, Cui Z-H, Bi W-L, Li J-W, Li B-Q, Ozudogru EA, Volk GM, Wang Q-C (2014a) Potential applications of cryogenic technologies to plant genetic improvement and pathogen eradication. Biotechnol Adv 32:583–595
Wang R-R, Gao X-X, Chen L, Huo L-Q, Li M-F, Wang Q-C (2014b) Shoot recovery and genetic integrity of Chrysanthemum morifolium shoot tips following cryopreservation by droplet-vitrification. Sci Hortic 176:330–339
Wang Sh, Hao L-J, Zhu J-J, Zhang Q-W, Wang Z-M, Zhang X, Song X-M (2014c) Study on the effects of sulfur fumigation on chemical constituents and antioxidant activity of Chrysanthemum morifolium cv. Hang-ju. Phytomedicine 21:773–777
Zhang Z, Skjeseth G, Elameen A, Haugslien S, Sivertsen A, Clarke JHL, Wang Q-C, Blystad D-R (2015) Field performance evaluation and genetic integrity assessment in Argyranthemum ‘Yellow Empire’ plants recovered from cryopreserved shoot tips. In Vitro Cell Dev Biol-Plant 51:505–513
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by M. Lambardi.
Rights and permissions
About this article
Cite this article
Bi, WL., Pan, C., Liu, J. et al. Greenhouse performance, genetic stability and biochemical compounds in Chrysanthemum morifolium ‘Hangju’ plants regenerated from cryopreserved shoot tips. Acta Physiol Plant 38, 268 (2016). https://doi.org/10.1007/s11738-016-2288-2
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11738-016-2288-2