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Optimization of pea (Pisum sativum) seeds hydropriming by application of response surface methodology

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Abstract

Evaluation of selected parameters viz. initial germination percentage (IGP), soaking duration (SD), process temperature, rotation speed (rpm) and air flow rate (AFR) was performed in this research investigation for hydropriming of Pea (Pisum sativum) seeds. Three seed lots having difference in their moisture content (14.94–28.04 % d.b) and germination percentage (60–80 %) were selected in this study. Procured seed lots were subjected to variable duration of accelerated aging (40 ± 1 °C, 100 % RH) to attain necessary seed lots for experimental run. Response surface methodology (Box–Behnken design) with five factors and three-level combination was adopted, and the independent variables are germination percentage (80, 70, 60), soaking duration (45, 60, 75 min), temperature (20, 25, 30 °C), rotation speed (320, 340, 360 rpm) and air flow rate (0.411, 0.548, 0.685 m3/min). Second order polynomial equation was fitted for analyzing the experimental data and data was also subjected to analysis of variance as a part of regression analysis. Process responses which were selected to evaluate the effect of hydropriming were moisture content after hydropriming, final germination percentage, seedling length, seedling dry weight, vigor indices (VI–I and VI–II) and electrical conductivity. Regression analysis suggested that models were significant for all process responses and using numerical optimization technique, the optimal solution found was 75 % IGP, 55 min SD, 20 °C temperature, 320 rpm and 0.50 m3/min AFR. Values predicted by model were found to be at par with the results of a confirmation experiment carried out at optimum conditions.

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Abbreviations

IGP:

Initial germination percentage

T:

Temperature

AFR:

Air flow rate

ANOVA:

Analysis of variance

FGP:

Final germination percentage

SDW:

Seedling dry weight

VI-II:

Vigor index-II

d.b.:

Dry basis

L2 :

Seed lot 2

MT:

Metric ton

RSM:

Response surface methodology

mS/cm:

Millisiemens/centimetre

SD:

Soaking duration

rpm:

Rotation speed

RH:

Relative humidity

MC:

Moisture content

SL:

Seedling length

VI–I:

Vigor index-I

EC:

Electrical conductivity

L 1 :

Seed lot 1

L 3 :

Seed lot 3

Mha:

Million hectare

BBD:

Box–Behnken design

min:

Minute

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Acknowledgments

The first author acknowledges University Grants Commission (UGC) for providing financial support in the form of Rajiv Gandhi National Fellowship. Also, gratitude is extended to Division of Agricultural Engineering, Seed Science and Technology, ICAR-IARI New Delhi and ICAR-NBPGR, New Delhi for providing research infrastructure and laboratories facilities.

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Authors and Affiliations

  1. Horticultural Crop Processing Division, Central Institute of Post-Harvest Engineering and Technology, Abohar, Punjab, 152116, India

    Manoj Kumar Mahawar & Kirti Jalgaonkar

  2. Division of Agricultural Engineering, Indian Agricultural Research Institute, New Delhi, 110012, India

    D. V. K. Samuel & J. P. Sinha

Authors
  1. Manoj Kumar Mahawar
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  2. D. V. K. Samuel
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  3. J. P. Sinha
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  4. Kirti Jalgaonkar
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Correspondence to Manoj Kumar Mahawar.

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Communicated by S. Weidner.

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Mahawar, M.K., Samuel, D.V.K., Sinha, J.P. et al. Optimization of pea (Pisum sativum) seeds hydropriming by application of response surface methodology. Acta Physiol Plant 38, 212 (2016). https://doi.org/10.1007/s11738-016-2234-3

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  • DOI: https://doi.org/10.1007/s11738-016-2234-3

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