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Comparative analysis of microRNAs and putative target genes in hybrid clone Paulownia ‘yuza 1’ under drought stress

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Abstract

Drought stress in plants often leads to reduced productivity and limited geographic distribution, which can affect human life and ecosystems. The responses of diploid and tetraploid Paulownia tomentosa × Paulownia fortunei to drought have been reported, but the effects of drought stress on the levels of microRNA (miRNA) expression have not been published so far. Here, we constructed four small RNA (sRNA) libraries and four corresponding degradome libraries of well-watered and severe drought-treated diploid and tetraploid plants to identify the miRNAs and their putative target genes in Paulownia ‘yuza 1’, a P. tomentosa × P. fortunei hybrid clone, by sRNA and degradome sequencing. The putative target genes of miRNAs were annotated with gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways. Three conserved and 21 novel miRNAs responsive to drought stress were found, in which 15 were identified as the main drought responsive miRNAs that conferred higher resistance in tetraploid than in diploid of Paulownia ‘yuza 1’. Our results will lay the foundation for investigating the roles of miRNAs in Paulownia and other trees in response to drought.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Grant No. 30271082, 30571496), by the Outstanding Talents Project of Henan Province (Grant No. 122101110700), and by the Transformation Project of the National Agricultural Scientific and Technological Achievement of China (Grant No. 2012GB2D000271).

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  1. Institute of Paulownia, Henan Agricultural University, Zhengzhou, 450002, Henan, People’s Republic of China

    Guoqiang Fan, Lu Yang, Minjie Deng, Suyan Niu, Zhenli Zhao, Yanpeng Dong & Yongsheng Li

  2. College of Forestry, Henan Agricultural University, Zhengzhou, 450002, Henan, People’s Republic of China

    Guoqiang Fan, Minjie Deng, Zhenli Zhao, Yanpeng Dong & Yongsheng Li

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  1. Guoqiang Fan
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  2. Lu Yang
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Correspondence to Guoqiang Fan.

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Fan, G., Yang, L., Deng, M. et al. Comparative analysis of microRNAs and putative target genes in hybrid clone Paulownia ‘yuza 1’ under drought stress. Acta Physiol Plant 38, 203 (2016). https://doi.org/10.1007/s11738-016-2225-4

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