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Acta Physiologiae Plantarum

, 38:52 | Cite as

Oil palm leaves and roots differ in physiological response, antioxidant enzyme activities and expression of stress-responsive genes upon exposure to drought stress

  • Azzreena Mohamad Azzeme
  • Siti Nor Akmar AbdullahEmail author
  • Maheran Abdul Aziz
  • Puteri Edaroyati Megat Wahab
Original Article

Abstract

The seedling stage is a critical period for survival under drought stress. To identify biochemical and molecular drought response changes, oil palm seedlings were exposed to different levels of drought severity. Total chlorophyll, total soluble protein and total proline content were measured while expression of stress responsive genes was quantified using qPCR. The diminishing total chlorophyll (chl) content and the ratio of chla to chlb (chla:chlb) were observed to be significant. The significant reduction of chla was closely related to photosystem II deficiency. Based on the effects of drought on chlorophyll content, the samples can be categorised into mild (7 days of water withholding; DWW), moderate (14 DWW) and severe (21, 28 and 35 DWW). Sample at 21 DWW was used to represent the severe stage. Genes encoding ethylene responsive binding protein, late embryogenesis abundant (LEA), dehydrin (DHN), cold-induced, heat shock protein 70 and metallothionein type 2 were differentially up-regulated in the leaves, while in the roots only LEA and DHN were up-regulated. The proline content increased gradually in both vegetative tissues, while the total soluble protein content was affected by increasing drought severity. The activity of catalase was highest in the roots at the severe drought stage, while guaicol peroxidase activity was shown to be highest in the leaves under mild drought. These findings provide new insights into stress tolerance mechanisms of oil palm seedlings and can be used to develop stress tolerant oil palm through classical breeding and genetic engineering.

Keywords

Elaeis guineensis Drought Transcription factor Stress responsive genes (SRGs) Enzymatic and non-enzymatic antioxidant Vegetative tissues 

Notes

Acknowledgments

We thank Universiti Putra Malaysia for providing the research grant.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2016

Authors and Affiliations

  • Azzreena Mohamad Azzeme
    • 1
    • 2
  • Siti Nor Akmar Abdullah
    • 1
    • 3
    Email author
  • Maheran Abdul Aziz
    • 1
    • 3
  • Puteri Edaroyati Megat Wahab
    • 4
  1. 1.Laboratory of Plantation Crops, Institute of Tropical AgricultureUniversiti Putra Malaysia (UPM)SerdangMalaysia
  2. 2.Department of Biochemistry, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra Malaysia (UPM)SerdangMalaysia
  3. 3.Department of Agriculture Technology, Faculty of AgricultureUniversiti Putra Malaysia (UPM)SerdangMalaysia
  4. 4.Department of Crop Sciences, Faculty of AgricultureUniversiti Putra Malaysia (UPM)SerdangMalaysia

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