Expression of ethylene biosynthetic and signaling genes in relation to ripening of banana fruit after cold storage

Abstract

Banana fruit are highly sensitive to chilling injury (CI), while the effect of different degrees of CI on the subsequent fruit ripening is largely unknown. In the present work, ripening characteristic of banana fruit after storage at 7 °C for 3 days or for 8 days, and expression levels of eight genes associated with ethylene biosynthetic and signaling, including MaACS1, MaACO1, MaERS1, MaERS3, and MaEIL14, were investigated. The results showed that banana fruit stored at 7 °C for 8 days exhibited more severe chilling symptoms than those at 7 °C for 3 days. Compared with banana fruit stored at 7 °C for 8 days, which showed abnormal ripening, more decrease in fruit firmness, while higher increase in ethylene production and hue angle were observed in banana fruit stored at 7 °C for 3 days, which could ripening normally. Moreover, gene expression profiles during ripening revealed that ethylene biosynthetic and signaling genes were differentially expressed in peel and pulp of banana fruit after storage at 7 °C for 3 days and 7 °C for 8 days. In the peel of fruit storage at 7 °C for 3 days, expression levels of MaACS1, MaACO1, MaEIL1, and MaEIL2 increased remarkably while MaERS3, MaEIL1, and MaEIL4 were enhanced in the fruit after storage at 7 °C for 8 days. In the pulp, with the exception of MaACO1 and MaERS3, expression levels of other genes did not exhibit a significant difference, between the banana fruit storage at 7 °C for 3 days and 7 °C for 8 days. Taken together, our results suggest that differential expression of ethylene biosynthetic and signaling genes such as MaERS3, MaACO1, and MaEIL2, may be related to ripening behavior of banana fruit with different degrees of CI after cold storage.

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Acknowledgments

This work was supported in part by the National Natural Science Foundation of China (grant no. 31160406) and Guangxi Natural Science Foundation (grant no. 2012GXNSFBA053058).

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Correspondence to Quanguang He.

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K. Hong and J. Xie contributed equally to this work.

Communicated by P. K. Nagar.

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Hong, K., Xie, J., Zou, R. et al. Expression of ethylene biosynthetic and signaling genes in relation to ripening of banana fruit after cold storage. Acta Physiol Plant 37, 252 (2015). https://doi.org/10.1007/s11738-015-2005-6

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Keywords

  • Banana fruit
  • Chilling injury
  • Ripening
  • Ethylene biosynthetic and signaling genes