Acta Physiologiae Plantarum

, 37:245 | Cite as

Modifications of cell wall pectin in tomato cell suspension in response to cadmium and zinc

  • Aurélie Muschitz
  • Catherine Riou
  • Jean-Claude Mollet
  • Vincent Gloaguen
  • Céline Faugeron
Original Article

Abstract

Retention of metal cations by the cell wall is a common process found in plants in response to stress induced by the presence of trace metals (TMs). In this study conducted on a tomato cell suspension culture, cadmium (Cd) and zinc (Zn) were added to the medium at maximal concentrations of 0.5 and 2 mM, respectively. We showed that around 50 % of Zn or Cd was confined into the cell wall of tomato cells. Besides, their accumulation in the cell wall increased with the exogenous concentration in the culture medium. Characterization of cell wall pectins showed a decrease in the highly methylesterified pectin fraction whereas the weakly methylesterified pectin remained stable in response to Cd. Moreover, a significant increase in the degree of methylesterification was observed in both fractions. This was probably associated to the reduced pectin methylesterase (PME) activity in the treated cells. Furthermore, linked to a reduction of pectin content we showed a reduced expression of the galacturonosyltransferase QUA1 gene whereas PME1 expression remained unchanged. Taking together, these data strongly suggest that pectin biosynthesis and its modification in the cell wall are strongly regulated in response to TM exposure in tomato cells.

Keywords

Trace elements Cell wall Pectins Tomato (Solanum lycopersicum L.) 

Abbreviations

dCW

Dry cell wall

DM

Degree of methylesterification

HG

Homogalacturonan

HM

Highly methylesterified

OE

Oxalate extract

PME

Pectin methylesterase

TM

Trace metal

UA

Uronic acid

WE

Water extract

WM

Weakly methylesterified pectins

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  • Aurélie Muschitz
    • 1
  • Catherine Riou
    • 1
  • Jean-Claude Mollet
    • 2
  • Vincent Gloaguen
    • 1
  • Céline Faugeron
    • 1
  1. 1.Laboratoire de Chimie des Substances NaturellesUniversité de Limoges, Faculté des Sciences et TechniquesLimoges CedexFrance
  2. 2.Laboratoire Glycobiologie et Matrice Extracellulaire Végétale, EA4358Normandie Université, IRIB, VASIMont-Saint-AignanFrance

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