Abstract
Retention of metal cations by the cell wall is a common process found in plants in response to stress induced by the presence of trace metals (TMs). In this study conducted on a tomato cell suspension culture, cadmium (Cd) and zinc (Zn) were added to the medium at maximal concentrations of 0.5 and 2 mM, respectively. We showed that around 50 % of Zn or Cd was confined into the cell wall of tomato cells. Besides, their accumulation in the cell wall increased with the exogenous concentration in the culture medium. Characterization of cell wall pectins showed a decrease in the highly methylesterified pectin fraction whereas the weakly methylesterified pectin remained stable in response to Cd. Moreover, a significant increase in the degree of methylesterification was observed in both fractions. This was probably associated to the reduced pectin methylesterase (PME) activity in the treated cells. Furthermore, linked to a reduction of pectin content we showed a reduced expression of the galacturonosyltransferase QUA1 gene whereas PME1 expression remained unchanged. Taking together, these data strongly suggest that pectin biosynthesis and its modification in the cell wall are strongly regulated in response to TM exposure in tomato cells.
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Abbreviations
- dCW:
-
Dry cell wall
- DM:
-
Degree of methylesterification
- HG:
-
Homogalacturonan
- HM:
-
Highly methylesterified
- OE:
-
Oxalate extract
- PME:
-
Pectin methylesterase
- TM:
-
Trace metal
- UA:
-
Uronic acid
- WE:
-
Water extract
- WM:
-
Weakly methylesterified pectins
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Acknowledgments
A.M. was supported by a post-doctoral fellowship from the Région Limousin. The authors are grateful to Dr. Michel Guilloton for critical reading of the manuscript and to Flavien Dardelle for his help on the immuno-blots. This article is dedicated to Professor Henri Morvan.
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Communicated by J. Kovacik.
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Muschitz, A., Riou, C., Mollet, JC. et al. Modifications of cell wall pectin in tomato cell suspension in response to cadmium and zinc. Acta Physiol Plant 37, 245 (2015). https://doi.org/10.1007/s11738-015-2000-y
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DOI: https://doi.org/10.1007/s11738-015-2000-y