Biochemical characterization of the primary metabolism and antioxidant defense systems of acidic and acidless citrus genotypes during the major stages of fruit growth

Acta Physiologiae Plantarum volume 37, Article number: 228 (2015) Cite this article

Abstract

Fruits are consumed not just for their taste but also for their nutritional value. The major primary metabolites in fruit are sugars and acids, whose contents change during fruit growth and determine ultimate fruit quality. Fruits are also a source of antioxidant metabolites, which are important to human health due to their role in reducing risk of cancer and cardiovascular diseases. Antioxidants are equally important in the plant as they help fight against oxidative stress. Here, we investigated the consequences of changes in the primary metabolism in acidic and acidless citrus genotypes during the major stages of fruit growth on the expression of antioxidant enzymes and the markers of cellular oxidation (hydrogen peroxide, malondialdehyde) in acidless (Iaffaoui orange and sweet lemon) and acidic (Salustiana orange and Villafranca lemon) citrus fruits. Glucose and fructose were the major sugars in the acidless lemon. Sucrose was the major sugar in the acidic lemon. Oranges shared a balance of glucose, fructose, and sucrose. Malic and citric acid concentrations were higher in acidic lemons than acidless fruits. Acidic genotypes had higher hydrogen peroxide concentrations than acidless genotypes, whereas MDA concentrations were higher in oranges than in lemons. Specific activities of ascorbate peroxidase, catalase, superoxide dismutase, and dehydroascorbate reductase were on the whole higher in acidic than acidless fruits. Principal component analysis revealed between-genotype divergence in antioxidant system, giving three groups: acidic lemons, acidless lemons, and oranges.

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Acknowledgments

The authors thank the Collectivité Territoriale de Corse (CTC) for providing financial support for this study.

Author information

Affiliations

  1. CNRS, UMR 6134 SPE, Laboratoire Biochimie and Biologie Moléculaire du Végétal, 20250, Corte, France

    Julie Oustric, Sandrine Antoine, Jean Giannettini, Liliane Berti & Jérémie Santini

  2. UMR AGAP Corse, Station INRA, 20230, San Giuliano, France

    Sandrine Antoine & François Luro

  3. Institut National de la Recherche Agronomique and Université de Bordeaux, Unité Mixte de Recherche 1332, Biologie du Fruit et Pathologie, 33883, Villenave-d’Ornon, France

    Yves Gibon

  4. Metabolomics Platform–Functional Genomics Centre Bordeaux, INRA Bordeaux, 71 av Edouard Bourlaux, 33140, Villenave-d’Ornon, France

    Yves Gibon

Authors
  1. Julie Oustric
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  2. Sandrine Antoine
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  3. Jean Giannettini
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  4. Yves Gibon
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  5. François Luro
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  6. Liliane Berti
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  7. Jérémie Santini
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Correspondence to Jérémie Santini.

Additional information

Communicated by P. K. Nagar.

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Oustric, J., Antoine, S., Giannettini, J. et al. Biochemical characterization of the primary metabolism and antioxidant defense systems of acidic and acidless citrus genotypes during the major stages of fruit growth. Acta Physiol Plant 37, 228 (2015). https://doi.org/10.1007/s11738-015-1982-9

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Keywords

  • Citrus
  • Sugars
  • Organic acids
  • Antioxidant system
  • Oxidative status